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Article: Using self-assembled nanomaterials to inhibit the formation of metastatic cancer stem cell colonies in vitro

TitleUsing self-assembled nanomaterials to inhibit the formation of metastatic cancer stem cell colonies in vitro
Authors
KeywordsNanomedicine
Prostate cancer
Self-assembling peptide
Stem cell
Issue Date2011
Citation
Cell Transplantation, 2011, v. 20 n. 1, p. 127-131 How to Cite?
AbstractThe isolation of cells with stem-like properties from prostate tumors suggests the presence of a cancer stem cell (CSC) population, which may account for the initiation, progression, and metastasis as well as drug resistance of the disease. We hypothesized that containing, or at least immobilizing, the CSCs in a nano-self-assembling material might help prevent prostate tumor progression or metastasis. CSCs were plated in three conditions: 1) placed in 1% concentration self-assembled peptide (SAP) preequilibrate with culture medium; 2) placed in 3% concentration SAP preequilibrate with culture medium; and 3) in nonadherent culture. All were grown for 14 days, after which the cells in both 1% and 3% concentrations were washed out of the SAP and grown for an additional 14 days. Here we report that CSCs from prostate cancer cell lines remained quiescent for more than 28 days when embedded in SAP. When the prostate CSCs were embedded in 1% and 3% SAP, most of the CSCs remained single cells 14 days after plating in a nonadherent plate; no prostaspheres could be detected 14 days after plating, suggesting that self-renewal was significantly suppressed. In the controls, prostate CSCs began to divide 1 day after plating in a nonadherent plate and prostaspheres were visible at day 10, indicating the active self-renewal property of the prostate CSCs. Our findings suggest that SAP can completely inhibit a prostate CSC from self-renewal while preserving its viability and CSC property. Therefore, SAP may be an effective nanomaterial for inhibiting cancer progression and metastasis to stop the progression during treatment and removal. Copyright © 2011 Cognizant Comm. Corp. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/149761
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

This work was supported by a grant from the University of Hong Kong Seed Fund Programme for Applied Research.

References

 

DC FieldValueLanguage
dc.contributor.authorLing, PMTen_HK
dc.contributor.authorCheung, SWHen_HK
dc.contributor.authorTay, DKCen_HK
dc.contributor.authorEllisBehnke, RGen_HK
dc.date.accessioned2012-06-26T05:58:11Z-
dc.date.available2012-06-26T05:58:11Z-
dc.date.issued2011en_HK
dc.identifier.citationCell Transplantation, 2011, v. 20 n. 1, p. 127-131en_HK
dc.identifier.issn0963-6897en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149761-
dc.description.abstractThe isolation of cells with stem-like properties from prostate tumors suggests the presence of a cancer stem cell (CSC) population, which may account for the initiation, progression, and metastasis as well as drug resistance of the disease. We hypothesized that containing, or at least immobilizing, the CSCs in a nano-self-assembling material might help prevent prostate tumor progression or metastasis. CSCs were plated in three conditions: 1) placed in 1% concentration self-assembled peptide (SAP) preequilibrate with culture medium; 2) placed in 3% concentration SAP preequilibrate with culture medium; and 3) in nonadherent culture. All were grown for 14 days, after which the cells in both 1% and 3% concentrations were washed out of the SAP and grown for an additional 14 days. Here we report that CSCs from prostate cancer cell lines remained quiescent for more than 28 days when embedded in SAP. When the prostate CSCs were embedded in 1% and 3% SAP, most of the CSCs remained single cells 14 days after plating in a nonadherent plate; no prostaspheres could be detected 14 days after plating, suggesting that self-renewal was significantly suppressed. In the controls, prostate CSCs began to divide 1 day after plating in a nonadherent plate and prostaspheres were visible at day 10, indicating the active self-renewal property of the prostate CSCs. Our findings suggest that SAP can completely inhibit a prostate CSC from self-renewal while preserving its viability and CSC property. Therefore, SAP may be an effective nanomaterial for inhibiting cancer progression and metastasis to stop the progression during treatment and removal. Copyright © 2011 Cognizant Comm. Corp. All rights reserved.en_HK
dc.languageengen_US
dc.relation.ispartofCell Transplantationen_HK
dc.subjectNanomedicineen_HK
dc.subjectProstate canceren_HK
dc.subjectSelf-assembling peptideen_HK
dc.subjectStem cellen_HK
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Proliferation - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshNanostructures - Chemistry - Toxicityen_US
dc.subject.meshNeoplasm Metastasisen_US
dc.subject.meshNeoplastic Stem Cells - Cytology - Drug Effectsen_US
dc.subject.meshPeptides - Toxicityen_US
dc.subject.meshProstatic Neoplasms - Pathology - Therapyen_US
dc.titleUsing self-assembled nanomaterials to inhibit the formation of metastatic cancer stem cell colonies in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailLing, PMT: patling@hkucc.hku.hken_HK
dc.identifier.emailCheung, SWH: sunnycwh@hku.hken_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailEllisBehnke, RG: rutledg@mit.eduen_HK
dc.identifier.authorityLing, PMT=rp00449en_HK
dc.identifier.authorityCheung, SWH=rp00246en_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.identifier.authorityEllisBehnke, RG=rp00252en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3727/096368910X532783en_HK
dc.identifier.pmid20887677-
dc.identifier.scopuseid_2-s2.0-79952520420en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79952520420&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue1en_HK
dc.identifier.spage127en_HK
dc.identifier.epage131en_HK
dc.identifier.isiWOS:000289014900014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLing, PMT=7102229780en_HK
dc.identifier.scopusauthoridCheung, SWH=36152058800en_HK
dc.identifier.scopusauthoridTay, DKC=55392935900en_HK
dc.identifier.scopusauthoridEllisBehnke, RG=8548055200en_HK
dc.identifier.citeulike8953021-

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