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Article: Identification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells
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TitleIdentification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells
 
AuthorsHu, H2 1
Zhang, XP2
Wang, YL2
Chua, CW1
Luk, SU1
Wong, YC1
Ling, MT1
Wang, XF2
Xu, KX2
 
Issue Date2011
 
CitationMolecular Medicine Reports, 2011, v. 4 n. 1, p. 9-16 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2010.380
 
AbstractStudies have shown that the expression of inhibitor of differentiation (Id-1) is increased in bladder cancer and is associated with drug resistance. S-allylmercaptocysteine (SAMC), a water-soluble component of garlic, is known to have a potent therapeutic effect on human cancer. The aim of this study was to investigate whether Id-1 expression mediates SAMC-induced cell death in bladder cancer cells. After generating stable Id-1-expressing and si-Id-1 transfectants in various bladder cancer cell lines, cell sensitivity to SAMC was compared by colony formation and MTT assays. The results indicated that Id-1 overexpression reduced the positive effect of SAMC on cell survival, while the inactivation of Id-1 increased cellular susceptibility to SAMC. Using DAPI staining, the apoptosis of bladder cancer cells induced by SAMC was shown to be negatively regulated by Id-1 expression. The expression of apoptosis-related proteins analyzed by Western blotting further supported the negative role of Id-1 in SAMC-induced apoptosis. Furthermore, by wound closure and type I collagen invasion assays, the inhibitory effect of SAMC on the invasion and migration of bladder cancer cells was found to be associated with the down-regulation of Id-1. Our results demonstrated that SAMC-induced apoptosis is associated with the Id-1 pathway, and that the inactivation of Id-1 enhances the ability of SAMC to inhibit the survival, invasion and migration of bladder cancer cells. These findings may lead to the development of novel therapeutic strategies for the treatment of bladder cancer.
 
ISSN1791-2997
2012 Impact Factor: 1.17
2012 SCImago Journal Rankings: 0.363
 
DOIhttp://dx.doi.org/10.3892/mmr.2010.380
 
ISI Accession Number IDWOS:000286301100002
Funding AgencyGrant Number
Peking University People's HospitalRDB2008-30
Funding Information:

This study was supported by the Peking University People's Hospital Research and Development Fund to Dr Ke Xin Xu (RDB2008-30).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHu, H
 
dc.contributor.authorZhang, XP
 
dc.contributor.authorWang, YL
 
dc.contributor.authorChua, CW
 
dc.contributor.authorLuk, SU
 
dc.contributor.authorWong, YC
 
dc.contributor.authorLing, MT
 
dc.contributor.authorWang, XF
 
dc.contributor.authorXu, KX
 
dc.date.accessioned2012-06-26T05:58:09Z
 
dc.date.available2012-06-26T05:58:09Z
 
dc.date.issued2011
 
dc.description.abstractStudies have shown that the expression of inhibitor of differentiation (Id-1) is increased in bladder cancer and is associated with drug resistance. S-allylmercaptocysteine (SAMC), a water-soluble component of garlic, is known to have a potent therapeutic effect on human cancer. The aim of this study was to investigate whether Id-1 expression mediates SAMC-induced cell death in bladder cancer cells. After generating stable Id-1-expressing and si-Id-1 transfectants in various bladder cancer cell lines, cell sensitivity to SAMC was compared by colony formation and MTT assays. The results indicated that Id-1 overexpression reduced the positive effect of SAMC on cell survival, while the inactivation of Id-1 increased cellular susceptibility to SAMC. Using DAPI staining, the apoptosis of bladder cancer cells induced by SAMC was shown to be negatively regulated by Id-1 expression. The expression of apoptosis-related proteins analyzed by Western blotting further supported the negative role of Id-1 in SAMC-induced apoptosis. Furthermore, by wound closure and type I collagen invasion assays, the inhibitory effect of SAMC on the invasion and migration of bladder cancer cells was found to be associated with the down-regulation of Id-1. Our results demonstrated that SAMC-induced apoptosis is associated with the Id-1 pathway, and that the inactivation of Id-1 enhances the ability of SAMC to inhibit the survival, invasion and migration of bladder cancer cells. These findings may lead to the development of novel therapeutic strategies for the treatment of bladder cancer.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationMolecular Medicine Reports, 2011, v. 4 n. 1, p. 9-16 [How to Cite?]
DOI: http://dx.doi.org/10.3892/mmr.2010.380
 
dc.identifier.doihttp://dx.doi.org/10.3892/mmr.2010.380
 
dc.identifier.epage16
 
dc.identifier.isiWOS:000286301100002
Funding AgencyGrant Number
Peking University People's HospitalRDB2008-30
Funding Information:

This study was supported by the Peking University People's Hospital Research and Development Fund to Dr Ke Xin Xu (RDB2008-30).

 
dc.identifier.issn1791-2997
2012 Impact Factor: 1.17
2012 SCImago Journal Rankings: 0.363
 
dc.identifier.issue1
 
dc.identifier.pmid21461556
 
dc.identifier.scopuseid_2-s2.0-78651404249
 
dc.identifier.spage9
 
dc.identifier.urihttp://hdl.handle.net/10722/149757
 
dc.identifier.volume4
 
dc.languageeng
 
dc.relation.ispartofMolecular Medicine Reports
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAntineoplastic Agents, Phytogenic - Pharmacology
 
dc.subject.meshCell Death - Drug Effects
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshCell Movement - Drug Effects
 
dc.subject.meshCysteine - Analogs & Derivatives - Pharmacology
 
dc.subject.meshGarlic - Chemistry
 
dc.subject.meshGene Expression Regulation, Neoplastic
 
dc.subject.meshHumans
 
dc.subject.meshInhibitor Of Differentiation Protein 1 - Genetics - Metabolism
 
dc.subject.meshUrinary Bladder Neoplasms - Drug Therapy - Genetics
 
dc.titleIdentification of a novel function of Id-1 in mediating the anticancer responses of SAMC, a water-soluble garlic derivative, in human bladder cancer cells
 
dc.typeArticle
 
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<contributor.author>Luk, SU</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Peking University