Article: Catalytic characteristics of CYP3A22-dependent mequindox detoxification

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TitleCatalytic characteristics of CYP3A22-dependent mequindox detoxification
AuthorsLiu, J2
Ge, X2
Ouyang, M2
Tang, X2
Wu, J2
Wang, J2
Jiang, J2
Huen, MSY1
Deng, Y2
KeywordsCarbonyl Reduction
Cyp3a22
Hydroxylation
Mequindox
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/catcom
CitationCatalysis Communications, 2011, v. 12 n. 7, p. 637-643 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.catcom.2010.12.029
AbstractThe anti-bacterial agent mequindox is widely used as an animal feed additive in China. Its potential effect on host cells, however, remains largely unknown. Here, we report that pig CYP3A22 represents an important metabolic enzyme in mequindox catalysis. Interestingly, we found that ectopic expression of CYP3A22 reversed the mequindox-induced G2 cell cycle accumulation in cultured pig hepatocytes. To further probe the catalytic mechanism of CYP3A22 in mequindox metabolism, we identified 2′-acetyl-hydroxylation as a major catalytic step of CYP3A22-dependent mequindox metabolism using LC-MS/MS spectrometry. Together, our study suggests that CYP3A22 plays an important role in mequindox metabolism via 2′-acetyl-hydroxylation. © 2010 Elsevier B.V. All rights reserved.
ISSN1566-7367
2011 Impact Factor: 2.986
2011 SCImago Journal Rankings: 0.178
DOIhttp://dx.doi.org/10.1016/j.catcom.2010.12.029
ISI Accession Number IDWOS:000287839600016
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2009CB118802
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Program for New Century Excellent Talents in UniversityNCET-08-0643
Guangdong Province Universities and Colleges Special Funds for Talents Introduction
Funding Information:

This work was supported by the National Basic Research Program of China (973 Program), No: 2009CB118802; the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2009); the Program for New Century Excellent Talents in University, No: NCET-08-0643; and the Guangdong Province Universities and Colleges Special Funds for Talents Introduction (2008).

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLiu, J
dc.contributor.authorGe, X
dc.contributor.authorOuyang, M
dc.contributor.authorTang, X
dc.contributor.authorWu, J
dc.contributor.authorWang, J
dc.contributor.authorJiang, J
dc.contributor.authorHuen, MSY
dc.contributor.authorDeng, Y
dc.date.accessioned2012-06-26T05:58:08Z
dc.date.available2012-06-26T05:58:08Z
dc.date.issued2011
dc.description.abstractThe anti-bacterial agent mequindox is widely used as an animal feed additive in China. Its potential effect on host cells, however, remains largely unknown. Here, we report that pig CYP3A22 represents an important metabolic enzyme in mequindox catalysis. Interestingly, we found that ectopic expression of CYP3A22 reversed the mequindox-induced G2 cell cycle accumulation in cultured pig hepatocytes. To further probe the catalytic mechanism of CYP3A22 in mequindox metabolism, we identified 2′-acetyl-hydroxylation as a major catalytic step of CYP3A22-dependent mequindox metabolism using LC-MS/MS spectrometry. Together, our study suggests that CYP3A22 plays an important role in mequindox metabolism via 2′-acetyl-hydroxylation. © 2010 Elsevier B.V. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCatalysis Communications, 2011, v. 12 n. 7, p. 637-643 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.catcom.2010.12.029
dc.identifier.citeulike8624091
dc.identifier.doihttp://dx.doi.org/10.1016/j.catcom.2010.12.029
dc.identifier.epage643
dc.identifier.isiWOS:000287839600016
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2009CB118802
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Program for New Century Excellent Talents in UniversityNCET-08-0643
Guangdong Province Universities and Colleges Special Funds for Talents Introduction
Funding Information:

This work was supported by the National Basic Research Program of China (973 Program), No: 2009CB118802; the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2009); the Program for New Century Excellent Talents in University, No: NCET-08-0643; and the Guangdong Province Universities and Colleges Special Funds for Talents Introduction (2008).

dc.identifier.issn1566-7367
2011 Impact Factor: 2.986
2011 SCImago Journal Rankings: 0.178
dc.identifier.issue7
dc.identifier.scopuseid_2-s2.0-78651097502
dc.identifier.spage637
dc.identifier.urihttp://hdl.handle.net/10722/149755
dc.identifier.volume12
dc.languageeng
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/catcom
dc.publisher.placeNetherlands
dc.relation.ispartofCatalysis Communications
dc.relation.referencesReferences in Scopus
dc.subjectCarbonyl Reduction
dc.subjectCyp3a22
dc.subjectHydroxylation
dc.subjectMequindox
dc.titleCatalytic characteristics of CYP3A22-dependent mequindox detoxification
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. South China Agricultural University