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Article: Catalytic characteristics of CYP3A22-dependent mequindox detoxification
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TitleCatalytic characteristics of CYP3A22-dependent mequindox detoxification
 
AuthorsLiu, J2
Ge, X2
Ouyang, M2
Tang, X2
Wu, J2
Wang, J2
Jiang, J2
Huen, MSY1
Deng, Y2
 
KeywordsCarbonyl Reduction
Cyp3a22
Hydroxylation
Mequindox
 
Issue Date2011
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/catcom
 
CitationCatalysis Communications, 2011, v. 12 n. 7, p. 637-643 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.catcom.2010.12.029
 
AbstractThe anti-bacterial agent mequindox is widely used as an animal feed additive in China. Its potential effect on host cells, however, remains largely unknown. Here, we report that pig CYP3A22 represents an important metabolic enzyme in mequindox catalysis. Interestingly, we found that ectopic expression of CYP3A22 reversed the mequindox-induced G2 cell cycle accumulation in cultured pig hepatocytes. To further probe the catalytic mechanism of CYP3A22 in mequindox metabolism, we identified 2′-acetyl-hydroxylation as a major catalytic step of CYP3A22-dependent mequindox metabolism using LC-MS/MS spectrometry. Together, our study suggests that CYP3A22 plays an important role in mequindox metabolism via 2′-acetyl-hydroxylation. © 2010 Elsevier B.V. All rights reserved.
 
ISSN1566-7367
2013 Impact Factor: 3.320
2013 SCImago Journal Rankings: 1.270
 
DOIhttp://dx.doi.org/10.1016/j.catcom.2010.12.029
 
ISI Accession Number IDWOS:000287839600016
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2009CB118802
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Program for New Century Excellent Talents in UniversityNCET-08-0643
Guangdong Province Universities and Colleges Special Funds for Talents Introduction
Funding Information:

This work was supported by the National Basic Research Program of China (973 Program), No: 2009CB118802; the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2009); the Program for New Century Excellent Talents in University, No: NCET-08-0643; and the Guangdong Province Universities and Colleges Special Funds for Talents Introduction (2008).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLiu, J
 
dc.contributor.authorGe, X
 
dc.contributor.authorOuyang, M
 
dc.contributor.authorTang, X
 
dc.contributor.authorWu, J
 
dc.contributor.authorWang, J
 
dc.contributor.authorJiang, J
 
dc.contributor.authorHuen, MSY
 
dc.contributor.authorDeng, Y
 
dc.date.accessioned2012-06-26T05:58:08Z
 
dc.date.available2012-06-26T05:58:08Z
 
dc.date.issued2011
 
dc.description.abstractThe anti-bacterial agent mequindox is widely used as an animal feed additive in China. Its potential effect on host cells, however, remains largely unknown. Here, we report that pig CYP3A22 represents an important metabolic enzyme in mequindox catalysis. Interestingly, we found that ectopic expression of CYP3A22 reversed the mequindox-induced G2 cell cycle accumulation in cultured pig hepatocytes. To further probe the catalytic mechanism of CYP3A22 in mequindox metabolism, we identified 2′-acetyl-hydroxylation as a major catalytic step of CYP3A22-dependent mequindox metabolism using LC-MS/MS spectrometry. Together, our study suggests that CYP3A22 plays an important role in mequindox metabolism via 2′-acetyl-hydroxylation. © 2010 Elsevier B.V. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCatalysis Communications, 2011, v. 12 n. 7, p. 637-643 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.catcom.2010.12.029
 
dc.identifier.citeulike8624091
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.catcom.2010.12.029
 
dc.identifier.epage643
 
dc.identifier.isiWOS:000287839600016
Funding AgencyGrant Number
National Basic Research Program of China (973 Program)2009CB118802
Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme
Program for New Century Excellent Talents in UniversityNCET-08-0643
Guangdong Province Universities and Colleges Special Funds for Talents Introduction
Funding Information:

This work was supported by the National Basic Research Program of China (973 Program), No: 2009CB118802; the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2009); the Program for New Century Excellent Talents in University, No: NCET-08-0643; and the Guangdong Province Universities and Colleges Special Funds for Talents Introduction (2008).

 
dc.identifier.issn1566-7367
2013 Impact Factor: 3.320
2013 SCImago Journal Rankings: 1.270
 
dc.identifier.issue7
 
dc.identifier.scopuseid_2-s2.0-78651097502
 
dc.identifier.spage637
 
dc.identifier.urihttp://hdl.handle.net/10722/149755
 
dc.identifier.volume12
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/catcom
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofCatalysis Communications
 
dc.relation.referencesReferences in Scopus
 
dc.subjectCarbonyl Reduction
 
dc.subjectCyp3a22
 
dc.subjectHydroxylation
 
dc.subjectMequindox
 
dc.titleCatalytic characteristics of CYP3A22-dependent mequindox detoxification
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. South China Agricultural University