Article: Co-expression of GAP-43 and nNOS in avulsed motoneurons and their potential role for motoneuron regeneration
| Title | Co-expression of GAP-43 and nNOS in avulsed motoneurons and their potential role for motoneuron regeneration | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | Yuan, Q1 3 Hu, B1 Chu, TH1 Su, H1 Zhang, W1 So, KF1 2 Lin, Z3 Wu, W1 2 | ||||||||
| Issue Date | 2010 | ||||||||
| Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yniox | ||||||||
| Citation | Nitric Oxide - Biology And Chemistry, 2010, v. 23 n. 4, p. 258-263 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.niox.2010.07.006 | ||||||||
| Abstract | Neuronal nitric oxide synthase (nNOS) is induced after axonal injury. The role of induced nNOS in injured neurons is not well established. In the present study, we investigated the co-expression of nNOS with GAP-43 in spinal motoneurons following axonal injury. The role of induced nNOS was discussed and evaluated. In normal rats, spinal motoneurons do not express nNOS or GAP-43. Following spinal root avulsion, expression of nNOS and GAP-43 were induced and colocalized in avulsed motoneurons. Reimplantation of avulsed roots resulted in a remarkable decrease of GAP-43- and nNOS-IR in the soma of the injured motoneurons. A number of GAP-43-IR regenerating motor axons were found in the reimplanted nerve. In contrast, the nNOS-IR was absent in reimplanted nerve. These results suggest that expression of GAP-43 in avulsed motoneurons is related to axonal regeneration whereas nNOS is not. © 2010 Elsevier Inc. | ||||||||
| ISSN | 1089-8603 2011 Impact Factor: 3.548 2011 SCImago Journal Rankings: 0.286 | ||||||||
| DOI | http://dx.doi.org/10.1016/j.niox.2010.07.006 | ||||||||
| ISI Accession Number ID | WOS:000283894500002
Funding Information: This study was supported by HKU Spinal Cord Injury Foundation and grants from the University of Hong Kong and Hong Kong Research Grants Council (RGC). | ||||||||
| References | References in Scopus |
| dc.contributor.author | Yuan, Q | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Hu, B | ||||||||
| dc.contributor.author | Chu, TH | ||||||||
| dc.contributor.author | Su, H | ||||||||
| dc.contributor.author | Zhang, W | ||||||||
| dc.contributor.author | So, KF | ||||||||
| dc.contributor.author | Lin, Z | ||||||||
| dc.contributor.author | Wu, W | ||||||||
| dc.date.accessioned | 2012-06-26T05:58:00Z | ||||||||
| dc.date.available | 2012-06-26T05:58:00Z | ||||||||
| dc.date.issued | 2010 | ||||||||
| dc.description.abstract | Neuronal nitric oxide synthase (nNOS) is induced after axonal injury. The role of induced nNOS in injured neurons is not well established. In the present study, we investigated the co-expression of nNOS with GAP-43 in spinal motoneurons following axonal injury. The role of induced nNOS was discussed and evaluated. In normal rats, spinal motoneurons do not express nNOS or GAP-43. Following spinal root avulsion, expression of nNOS and GAP-43 were induced and colocalized in avulsed motoneurons. Reimplantation of avulsed roots resulted in a remarkable decrease of GAP-43- and nNOS-IR in the soma of the injured motoneurons. A number of GAP-43-IR regenerating motor axons were found in the reimplanted nerve. In contrast, the nNOS-IR was absent in reimplanted nerve. These results suggest that expression of GAP-43 in avulsed motoneurons is related to axonal regeneration whereas nNOS is not. © 2010 Elsevier Inc. | ||||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||||
| dc.identifier.citation | Nitric Oxide - Biology And Chemistry, 2010, v. 23 n. 4, p. 258-263 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.niox.2010.07.006 | ||||||||
| dc.identifier.citeulike | 7587115 | ||||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.niox.2010.07.006 | ||||||||
| dc.identifier.epage | 263 | ||||||||
| dc.identifier.hkuros | 175293 | ||||||||
| dc.identifier.isi | WOS:000283894500002
Funding Information: This study was supported by HKU Spinal Cord Injury Foundation and grants from the University of Hong Kong and Hong Kong Research Grants Council (RGC). | ||||||||
| dc.identifier.issn | 1089-8603 2011 Impact Factor: 3.548 2011 SCImago Journal Rankings: 0.286 | ||||||||
| dc.identifier.issue | 4 | ||||||||
| dc.identifier.pmid | 20667480 | ||||||||
| dc.identifier.scopus | eid_2-s2.0-77958153558 | ||||||||
| dc.identifier.spage | 258 | ||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/149750 | ||||||||
| dc.identifier.volume | 23 | ||||||||
| dc.language | eng | ||||||||
| dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yniox | ||||||||
| dc.publisher.place | United States | ||||||||
| dc.relation.ispartof | Nitric Oxide - Biology and Chemistry | ||||||||
| dc.relation.references | References in Scopus | ||||||||
| dc.subject.mesh | Animals | ||||||||
| dc.subject.mesh | Axons - Metabolism | ||||||||
| dc.subject.mesh | Disease Models, Animal | ||||||||
| dc.subject.mesh | Female | ||||||||
| dc.subject.mesh | Gap-43 Protein - Metabolism | ||||||||
| dc.subject.mesh | Motor Neurons - Enzymology - Metabolism | ||||||||
| dc.subject.mesh | Nerve Regeneration | ||||||||
| dc.subject.mesh | Nitric Oxide Synthase Type I - Metabolism | ||||||||
| dc.subject.mesh | Rats | ||||||||
| dc.subject.mesh | Rats, Sprague-Dawley | ||||||||
| dc.title | Co-expression of GAP-43 and nNOS in avulsed motoneurons and their potential role for motoneuron regeneration | ||||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- Jinan University
- Chinese University of Hong Kong