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Article: Adrenomedullin regulates sperm motility and oviductal ciliary beat via cyclic adenosine 5′-monophosphate/protein kinase A and nitric oxide

TitleAdrenomedullin regulates sperm motility and oviductal ciliary beat via cyclic adenosine 5′-monophosphate/protein kinase A and nitric oxide
Authors
Issue Date2010
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2010, v. 151 n. 7, p. 3339-3347 How to Cite?
AbstractCilium and flagellum beating are important in reproduction and defects in their motion are associated with ectopic pregnancy and infertility. Adrenomedullin (ADM) is a polypeptide present in the reproductive system. This report demonstrates a novel action of ADM in enhancing the flagellar/ ciliary beating of human spermatozoa and rat oviductal ciliated cells. At the concentration found in the seminal plasma, it increases the progressive motility of spermatozoa. ADM binds to its classical receptor, calcitonin receptor-like receptor/receptor activity-modifying protein complex on spermatozoa. ADM treatment increases the protein kinase A activities, the cyclic adenosine monophosphate, and nitric oxide levels of spermatozoa and oviductal cells. Pharmacological activators and inhibitors confirmed that the ADM-induced flagella/ciliary beating was protein kinase A dependent. Whereas nitric oxide donors had no effect on sperm motility, they potentiated the motility-inducing action of protein kinase A activators, demonstrating for the first time the synergistic action of nitric oxide and protein kinase A signaling in flagellar/ciliary beating. The ADM-induced motility enhancement effect in spermatozoa also depended on the up-regulation of intracellular calcium, a known key regulator of sperm motility and ciliary beating. In conclusion, ADM is a common activator of flagellar/ciliary beating. The study provides a physiological basis on possible use of ADM as a fertility regulation drug. Copyright © 2010 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/149746
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
Funding AgencyGrant Number
Committee on Research and Conference Grant
University of Hong Kong
Hong Kong Research Grant CouncilHKU7578/06M
Funding Information:

This work was supported in part by grants from the Committee on Research and Conference Grant, The University of Hong Kong, and the Hong Kong Research Grant Council (Grant HKU7578/06M).

References

 

DC FieldValueLanguage
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorLiao, Sen_HK
dc.contributor.authorLam, KKWen_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorHo, JCMen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorYao, YQen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-06-26T05:57:56Z-
dc.date.available2012-06-26T05:57:56Z-
dc.date.issued2010en_HK
dc.identifier.citationEndocrinology, 2010, v. 151 n. 7, p. 3339-3347en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149746-
dc.description.abstractCilium and flagellum beating are important in reproduction and defects in their motion are associated with ectopic pregnancy and infertility. Adrenomedullin (ADM) is a polypeptide present in the reproductive system. This report demonstrates a novel action of ADM in enhancing the flagellar/ ciliary beating of human spermatozoa and rat oviductal ciliated cells. At the concentration found in the seminal plasma, it increases the progressive motility of spermatozoa. ADM binds to its classical receptor, calcitonin receptor-like receptor/receptor activity-modifying protein complex on spermatozoa. ADM treatment increases the protein kinase A activities, the cyclic adenosine monophosphate, and nitric oxide levels of spermatozoa and oviductal cells. Pharmacological activators and inhibitors confirmed that the ADM-induced flagella/ciliary beating was protein kinase A dependent. Whereas nitric oxide donors had no effect on sperm motility, they potentiated the motility-inducing action of protein kinase A activators, demonstrating for the first time the synergistic action of nitric oxide and protein kinase A signaling in flagellar/ciliary beating. The ADM-induced motility enhancement effect in spermatozoa also depended on the up-regulation of intracellular calcium, a known key regulator of sperm motility and ciliary beating. In conclusion, ADM is a common activator of flagellar/ciliary beating. The study provides a physiological basis on possible use of ADM as a fertility regulation drug. Copyright © 2010 by The Endocrine Society.en_HK
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.titleAdrenomedullin regulates sperm motility and oviductal ciliary beat via cyclic adenosine 5′-monophosphate/protein kinase A and nitric oxideen_HK
dc.typeArticleen_HK
dc.identifier.emailChiu, PCN: pchiucn@hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailHo, JCM: jhocm@hku.hken_HK
dc.identifier.emailHo, PC: pcho@hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityHo, JCM=rp00258en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1210/en.2010-0077en_HK
dc.identifier.pmid20444935-
dc.identifier.scopuseid_2-s2.0-77954597743en_HK
dc.identifier.hkuros171842-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954597743&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume151en_HK
dc.identifier.issue7en_HK
dc.identifier.spage3339en_HK
dc.identifier.epage3347en_HK
dc.identifier.eissn1945-7170-
dc.identifier.isiWOS:000279049300037-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridLiao, S=36170881600en_HK
dc.identifier.scopusauthoridLam, KKW=25637362300en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK
dc.identifier.scopusauthoridHo, JCM=7402649981en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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