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Article: Protective effects of pinostilbene, a resveratrol methylated derivative, against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells

TitleProtective effects of pinostilbene, a resveratrol methylated derivative, against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells
Authors
Keywords6-Hydroxydopamine
Neuroprotection
Parkinson's disease
Pinostilbene
Resveratrol
Issue Date2010
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbio
Citation
Journal Of Nutritional Biochemistry, 2010, v. 21 n. 6, p. 482-489 How to Cite?
AbstractResveratrol (3,4',5-trans-trihydroxystilbene) is a phytoalexin with emerging lines of evidence supporting its beneficial effects on cardiovascular systems and inhibition of carcinogenesis. It has also been reported that certain methylated resveratrol derivatives are more effective than resveratrol in the prevention/treatment of cancer. However, little is known about the impact of resveratrol and its derivatives on the development of Parkinson's disease. In this study, we compared the neuroprotective effects of resveratrol with four methylated (fully or partially) resveratrol derivatives against parkinsonian mimetic 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in SH-SY5Y cells. Release of lactate dehydrogenase and activity of caspase-3 triggered by 6-OHDA were significantly reduced by resveratrol and one of the methylated derivatives, pinostilbene (3,4'-dihydroxy-5-methoxystilbene), in a dose-dependent manner. In addition, pinostilbene exerted a potent neuroprotective effect with a wider effective concentration range than resveratrol. By using high-performance liquid chromatography, we found that uptake of pinostilbene into SH-SY5Y cells was significantly higher than that of resveratrol. Enhanced bioavailability may thus be a major factor contributing to the neuroprotective activity of pinostilbene. Moreover, Western blot analysis demonstrated that pinostilbene markedly attenuated the phosphorylation of JNK and c-Jun triggered by 6-OHDA. Besides, mammalian target of rapamycin kinase may be an intracellular target accounting for the neuroprotective effects of pinostilbene. Our findings demonstrate the potential of methylated stilbenes in neuroprotection and provide important information for further research in this field. © 2010 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/149743
ISSN
2014 Impact Factor: 3.794
2014 SCImago Journal Rankings: 1.370
ISI Accession Number ID
Funding AgencyGrant Number
HKU200711159028
University of Hong Kong
Funding Information:

The study is supported by HKU Strategic Theme Research on Drug Discovery, HKU Seed Funding for Basic Research (200711159028) to R. C.-C.C. J.C., HI. and K.-W.C. are supported by a postgraduate studentship, and M.-S.Y. is supported by a postdoctoral fellowship from the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorChao, Jen_US
dc.contributor.authorLi, Hen_US
dc.contributor.authorCheng, KWen_US
dc.contributor.authorYu, MSen_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorWang, Men_US
dc.date.accessioned2012-06-26T05:57:54Z-
dc.date.available2012-06-26T05:57:54Z-
dc.date.issued2010en_US
dc.identifier.citationJournal Of Nutritional Biochemistry, 2010, v. 21 n. 6, p. 482-489en_US
dc.identifier.issn0955-2863en_US
dc.identifier.urihttp://hdl.handle.net/10722/149743-
dc.description.abstractResveratrol (3,4',5-trans-trihydroxystilbene) is a phytoalexin with emerging lines of evidence supporting its beneficial effects on cardiovascular systems and inhibition of carcinogenesis. It has also been reported that certain methylated resveratrol derivatives are more effective than resveratrol in the prevention/treatment of cancer. However, little is known about the impact of resveratrol and its derivatives on the development of Parkinson's disease. In this study, we compared the neuroprotective effects of resveratrol with four methylated (fully or partially) resveratrol derivatives against parkinsonian mimetic 6-hydroxydopamine (6-OHDA)-induced neurotoxicity in SH-SY5Y cells. Release of lactate dehydrogenase and activity of caspase-3 triggered by 6-OHDA were significantly reduced by resveratrol and one of the methylated derivatives, pinostilbene (3,4'-dihydroxy-5-methoxystilbene), in a dose-dependent manner. In addition, pinostilbene exerted a potent neuroprotective effect with a wider effective concentration range than resveratrol. By using high-performance liquid chromatography, we found that uptake of pinostilbene into SH-SY5Y cells was significantly higher than that of resveratrol. Enhanced bioavailability may thus be a major factor contributing to the neuroprotective activity of pinostilbene. Moreover, Western blot analysis demonstrated that pinostilbene markedly attenuated the phosphorylation of JNK and c-Jun triggered by 6-OHDA. Besides, mammalian target of rapamycin kinase may be an intracellular target accounting for the neuroprotective effects of pinostilbene. Our findings demonstrate the potential of methylated stilbenes in neuroprotection and provide important information for further research in this field. © 2010 Elsevier Inc.en_US
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jnutbioen_US
dc.relation.ispartofJournal of Nutritional Biochemistryen_US
dc.rightsThe Journal of Nutritional Biochemistry. Copyright © Elsevier Inc.-
dc.subject6-Hydroxydopamine-
dc.subjectNeuroprotection-
dc.subjectParkinson's disease-
dc.subjectPinostilbene-
dc.subjectResveratrol-
dc.subject.meshCaspase 3 - Metabolismen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFree Radical Scavengers - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshL-Lactate Dehydrogenase - Metabolismen_US
dc.subject.meshMethylationen_US
dc.subject.meshModels, Chemicalen_US
dc.subject.meshNeurons - Drug Effects - Metabolismen_US
dc.subject.meshNeurotoxins - Metabolismen_US
dc.subject.meshOxidopamine - Pharmacologyen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshStilbenes - Chemistry - Pharmacologyen_US
dc.titleProtective effects of pinostilbene, a resveratrol methylated derivative, against 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cellsen_US
dc.typeArticleen_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.emailWang, M:mfwang@hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.identifier.authorityWang, M=rp00800en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jnutbio.2009.02.004en_US
dc.identifier.pmid19443200-
dc.identifier.scopuseid_2-s2.0-77952882393en_US
dc.identifier.hkuros170232-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952882393&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume21en_US
dc.identifier.issue6en_US
dc.identifier.spage482en_US
dc.identifier.epage489en_US
dc.identifier.isiWOS:000278149400004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChao, J=24558959000en_US
dc.identifier.scopusauthoridLi, H=25958185900en_US
dc.identifier.scopusauthoridCheng, KW=12141247000en_US
dc.identifier.scopusauthoridYu, MS=35346047600en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US
dc.identifier.scopusauthoridWang, M=7406691844en_US
dc.identifier.citeulike5368446-

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