Article: Human γδ T cells: A lymphoid lineage cell capable of professional phagocytosis

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TitleHuman γδ T cells: A lymphoid lineage cell capable of professional phagocytosis
AuthorsWu, Y
Wu, W1 2
Wong, WM1 2
Ward, E
Thrasher, AJ
Goldblatt, D3
Osman, M
Digard, P4
Canaday, DH5
Gustafsson, K
Issue Date2009
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
CitationJournal Of Immunology, 2009, v. 183 n. 9, p. 5622-5629 [How to Cite?]
DOI: http://dx.doi.org/10.4049/jimmunol.0901772
AbstractProfessional phagocytosis in mammals is considered to be performed exclusively by myeloid cell types. In this study, we demonstrate, for the first time, that a mammalian lymphocyte subset can operate as a professional phagocyte. By using confocal microscopy, transmission electron microscopy, and functional Ag presentation assays, we find that freshly isolated human peripheral blood γδ T cells can phagocytose Escherichia coli and 1 μm synthetic beads via Ab opsonization and CD16 (FcγRIII), leading to Ag processing and presentation on MHC class II. In contrast, other CD16 + lymphocytes, i.e., CD16 +/CD56 + NK cells, were not capable of such functions. These findings of distinct myeloid characteristics in γδ T cells strongly support the suggestion that γδ T cells are evolutionarily ancient lymphocytes and have implications for our understanding of their role in transitional immunity and the control of infectious diseases and cancer. Copyright © 2009 by The American Association of Immunologists, Inc.
ISSN0022-1767
2011 Impact Factor: 5.788
2011 SCImago Journal Rankings: 1.099
DOIhttp://dx.doi.org/10.4049/jimmunol.0901772
ISI Accession Number IDWOS:000271488500025
Funding AgencyGrant Number
Jean Shanks Foundation
Funding Information:

This work was supported by the Jean Shanks Foundation.

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWu, Y
dc.contributor.authorWu, W
dc.contributor.authorWong, WM
dc.contributor.authorWard, E
dc.contributor.authorThrasher, AJ
dc.contributor.authorGoldblatt, D
dc.contributor.authorOsman, M
dc.contributor.authorDigard, P
dc.contributor.authorCanaday, DH
dc.contributor.authorGustafsson, K
dc.date.accessioned2012-06-26T05:57:53Z
dc.date.available2012-06-26T05:57:53Z
dc.date.issued2009
dc.description.abstractProfessional phagocytosis in mammals is considered to be performed exclusively by myeloid cell types. In this study, we demonstrate, for the first time, that a mammalian lymphocyte subset can operate as a professional phagocyte. By using confocal microscopy, transmission electron microscopy, and functional Ag presentation assays, we find that freshly isolated human peripheral blood γδ T cells can phagocytose Escherichia coli and 1 μm synthetic beads via Ab opsonization and CD16 (FcγRIII), leading to Ag processing and presentation on MHC class II. In contrast, other CD16 + lymphocytes, i.e., CD16 +/CD56 + NK cells, were not capable of such functions. These findings of distinct myeloid characteristics in γδ T cells strongly support the suggestion that γδ T cells are evolutionarily ancient lymphocytes and have implications for our understanding of their role in transitional immunity and the control of infectious diseases and cancer. Copyright © 2009 by The American Association of Immunologists, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Immunology, 2009, v. 183 n. 9, p. 5622-5629 [How to Cite?]
DOI: http://dx.doi.org/10.4049/jimmunol.0901772
dc.identifier.doihttp://dx.doi.org/10.4049/jimmunol.0901772
dc.identifier.epage5629
dc.identifier.isiWOS:000271488500025
Funding AgencyGrant Number
Jean Shanks Foundation
Funding Information:

This work was supported by the Jean Shanks Foundation.

dc.identifier.issn0022-1767
2011 Impact Factor: 5.788
2011 SCImago Journal Rankings: 1.099
dc.identifier.issue9
dc.identifier.pmid19843947
dc.identifier.scopuseid_2-s2.0-77952704343
dc.identifier.spage5622
dc.identifier.urihttp://hdl.handle.net/10722/149742
dc.identifier.volume183
dc.languageeng
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Immunology
dc.relation.referencesReferences in Scopus
dc.subject.meshAmino Acid Sequence
dc.subject.meshAnimals
dc.subject.meshAntigen Presentation - Immunology
dc.subject.meshCell Line
dc.subject.meshCell Line, Transformed
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Lineage - Immunology
dc.subject.meshCoculture Techniques
dc.subject.meshEscherichia Coli - Immunology
dc.subject.meshHla-A Antigens - Immunology - Metabolism
dc.subject.meshHla-Drb1 Chains
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshMice, Transgenic
dc.subject.meshMicrospheres
dc.subject.meshMolecular Sequence Data
dc.subject.meshOpsonin Proteins - Metabolism
dc.subject.meshPhagocytosis - Immunology
dc.subject.meshReceptors, Antigen, T-Cell, Gamma-Delta - Biosynthesis - Physiology
dc.subject.meshReceptors, Igg - Physiology
dc.subject.meshT-Lymphocyte Subsets - Immunology - Metabolism - Ultrastructure
dc.subject.meshViral Matrix Proteins - Immunology - Metabolism
dc.titleHuman γδ T cells: A lymphoid lineage cell capable of professional phagocytosis
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
  3. UCL Institute of Child Health
  4. University of Cambridge
  5. Case Western Reserve University