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Article: Synaptic input of ON-bipolar cells onto the dopaminergic neurons of the mouse retina

TitleSynaptic input of ON-bipolar cells onto the dopaminergic neurons of the mouse retina
Authors
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 2010, v. 518 n. 11, p. 2035-2050 How to Cite?
AbstractIn the retina, dopamine fulfills a crucial role in neural adaptation to photopic illumination, but the pathway that carries cone signals to the dopaminergic amacrine (DA) cells was controversial. We identified the site of ON-cone bipolar input onto DA cells in transgenic mice in which both types of catecholaminergic amacrine (CA) cells were labeled with green fluorescent protein or human placental alkaline phosphatase (PLAP). In confocal Z series of retinal whole mounts stained with antibodies to tyrosine hydroxylase (TH), DA cells gave rise to varicose processes that descended obliquely through the scleral half of the inner plexiform layer (IPL) and formed a loose, tangential plexus in the middle of this layer. Comparison with the distribution of the dendrites of type 2 CA cells and examination of neurobiotininjected DA cells proved that their vitreal processes were situated in stratum S3 of the IPL. Electron microscope demonstration of PLAP activity showed that bipolar cell endings in S3 established ribbon synapses onto a postsynaptic dyad in which one or both processes were labeled by a precipitate of lead phosphate and therefore belonged to DA cells. In places, the postsynaptic DA cell processes returned a reciprocal synapse onto the bipolar endings. Confocal images of sections stained with antibodies to TH, kinesin Kif3a, which labels synaptic ribbons, and glutamate or GABAA receptors, confirmed that ribbon-containing endings made glutamatergic synapses onto DA cells processes in S3 and received from them GABAergic synapses. The presynaptic ON-bipolar cells most likely belonged to the CB3 (type 5) variety. © 2010 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/149741
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthEY01344
EY017169
Funding Information:

Grant sponsor: National Institutes of Health; Grant numbers: EY01344, EY017169; R.H.M. is a Senior Investigator of Research to Prevent Blindness.

References

 

DC FieldValueLanguage
dc.contributor.authorContini, Men_US
dc.contributor.authorLin, Ben_US
dc.contributor.authorKobayashi, Ken_US
dc.contributor.authorOkano, Hen_US
dc.contributor.authorMasland, RHen_US
dc.contributor.authorRaviola, Een_US
dc.date.accessioned2012-06-26T05:57:52Z-
dc.date.available2012-06-26T05:57:52Z-
dc.date.issued2010en_US
dc.identifier.citationJournal Of Comparative Neurology, 2010, v. 518 n. 11, p. 2035-2050en_US
dc.identifier.issn0021-9967en_US
dc.identifier.urihttp://hdl.handle.net/10722/149741-
dc.description.abstractIn the retina, dopamine fulfills a crucial role in neural adaptation to photopic illumination, but the pathway that carries cone signals to the dopaminergic amacrine (DA) cells was controversial. We identified the site of ON-cone bipolar input onto DA cells in transgenic mice in which both types of catecholaminergic amacrine (CA) cells were labeled with green fluorescent protein or human placental alkaline phosphatase (PLAP). In confocal Z series of retinal whole mounts stained with antibodies to tyrosine hydroxylase (TH), DA cells gave rise to varicose processes that descended obliquely through the scleral half of the inner plexiform layer (IPL) and formed a loose, tangential plexus in the middle of this layer. Comparison with the distribution of the dendrites of type 2 CA cells and examination of neurobiotininjected DA cells proved that their vitreal processes were situated in stratum S3 of the IPL. Electron microscope demonstration of PLAP activity showed that bipolar cell endings in S3 established ribbon synapses onto a postsynaptic dyad in which one or both processes were labeled by a precipitate of lead phosphate and therefore belonged to DA cells. In places, the postsynaptic DA cell processes returned a reciprocal synapse onto the bipolar endings. Confocal images of sections stained with antibodies to TH, kinesin Kif3a, which labels synaptic ribbons, and glutamate or GABAA receptors, confirmed that ribbon-containing endings made glutamatergic synapses onto DA cells processes in S3 and received from them GABAergic synapses. The presynaptic ON-bipolar cells most likely belonged to the CB3 (type 5) variety. © 2010 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_US
dc.relation.ispartofJournal of Comparative Neurologyen_US
dc.subject.meshAlkaline Phosphatase - Genetics - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDopamine - Metabolismen_US
dc.subject.meshGpi-Linked Proteinsen_US
dc.subject.meshGreen Fluorescent Proteins - Genetics - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshIsoenzymes - Genetics - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshNeurons - Cytology - Metabolismen_US
dc.subject.meshRetina - Cytology - Metabolismen_US
dc.subject.meshRetinal Bipolar Cells - Cytology - Metabolismen_US
dc.subject.meshSynapses - Metabolism - Ultrastructureen_US
dc.subject.meshTyrosine 3-Monooxygenase - Genetics - Metabolismen_US
dc.titleSynaptic input of ON-bipolar cells onto the dopaminergic neurons of the mouse retinaen_US
dc.typeArticleen_US
dc.identifier.emailLin, B:blin@hku.hken_US
dc.identifier.authorityLin, B=rp01356en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/cne.22320en_US
dc.identifier.pmid20394057-
dc.identifier.scopuseid_2-s2.0-77952133519en_US
dc.identifier.hkuros228171-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952133519&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume518en_US
dc.identifier.issue11en_US
dc.identifier.spage2035en_US
dc.identifier.epage2050en_US
dc.identifier.eissn1096-9861-
dc.identifier.isiWOS:000277292700010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridContini, M=7005062723en_US
dc.identifier.scopusauthoridLin, B=36165916900en_US
dc.identifier.scopusauthoridKobayashi, K=26643362800en_US
dc.identifier.scopusauthoridOkano, H=7201660094en_US
dc.identifier.scopusauthoridMasland, RH=7007167900en_US
dc.identifier.scopusauthoridRaviola, E=7003783467en_US

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