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Article: Up-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model
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TitleUp-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model
 
AuthorsChiu, K1
Zhou, Y1
Yeung, SC1
Lok, CKM1
Chan, OOC1
Chang, RCC1 2
So, KF1 2
Chiu, JF1
 
Issue Date2010
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
 
CitationJournal Of Cellular Biochemistry, 2010, v. 110 n. 2, p. 311-320 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcb.22539
 
AbstractWolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of-wolfberry extract-LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal βB2-crystallin. © 2010 Wiley-Liss, Inc.
 
ISSN0730-2312
2012 Impact Factor: 3.062
2012 SCImago Journal Rankings: 1.261
 
DOIhttp://dx.doi.org/10.1002/jcb.22539
 
ISI Accession Number IDWOS:000277482700006
Funding AgencyGrant Number
University of Hong Kong Foundation for Educational Development and Research Limited
Azalea (1972) Endowment Fund
Funding Information:

This study was supported by funding from the Jessie Ho Professorship in Neuroscience (The University of Hong Kong Foundation for Educational Development and Research Limited, and donation from Mr. George Ho), and the Azalea (1972) Endowment Fund.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChiu, K
 
dc.contributor.authorZhou, Y
 
dc.contributor.authorYeung, SC
 
dc.contributor.authorLok, CKM
 
dc.contributor.authorChan, OOC
 
dc.contributor.authorChang, RCC
 
dc.contributor.authorSo, KF
 
dc.contributor.authorChiu, JF
 
dc.date.accessioned2012-06-26T05:57:52Z
 
dc.date.available2012-06-26T05:57:52Z
 
dc.date.issued2010
 
dc.description.abstractWolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of-wolfberry extract-LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal βB2-crystallin. © 2010 Wiley-Liss, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Cellular Biochemistry, 2010, v. 110 n. 2, p. 311-320 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcb.22539
 
dc.identifier.doihttp://dx.doi.org/10.1002/jcb.22539
 
dc.identifier.epage320
 
dc.identifier.hkuros170567
 
dc.identifier.isiWOS:000277482700006
Funding AgencyGrant Number
University of Hong Kong Foundation for Educational Development and Research Limited
Azalea (1972) Endowment Fund
Funding Information:

This study was supported by funding from the Jessie Ho Professorship in Neuroscience (The University of Hong Kong Foundation for Educational Development and Research Limited, and donation from Mr. George Ho), and the Azalea (1972) Endowment Fund.

 
dc.identifier.issn0730-2312
2012 Impact Factor: 3.062
2012 SCImago Journal Rankings: 1.261
 
dc.identifier.issue2
 
dc.identifier.pmid20336662
 
dc.identifier.scopuseid_2-s2.0-77952066508
 
dc.identifier.spage311
 
dc.identifier.urihttp://hdl.handle.net/10722/149740
 
dc.identifier.volume110
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Cellular Biochemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshBlotting, Western
 
dc.subject.meshCell Survival - Drug Effects
 
dc.subject.meshCrystallins - Metabolism
 
dc.subject.meshDisease Models, Animal
 
dc.subject.meshElectrophoresis, Gel, Two-Dimensional
 
dc.subject.meshFemale
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshIntraocular Pressure
 
dc.subject.meshLycium - Chemistry
 
dc.subject.meshNeuroprotective Agents - Pharmacology
 
dc.subject.meshOcular Hypertension - Pathology - Physiopathology
 
dc.subject.meshPlant Extracts - Pharmacology
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshRetinal Ganglion Cells - Drug Effects - Metabolism
 
dc.subject.meshUp-Regulation
 
dc.titleUp-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong