File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Up-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model

TitleUp-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model
Authors
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2010, v. 110 n. 2, p. 311-320 How to Cite?
Abstract
Wolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of-wolfberry extract-LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal βB2-crystallin. © 2010 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/149740
ISSN
2013 Impact Factor: 3.368
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong Foundation for Educational Development and Research Limited
Azalea (1972) Endowment Fund
Funding Information:

This study was supported by funding from the Jessie Ho Professorship in Neuroscience (The University of Hong Kong Foundation for Educational Development and Research Limited, and donation from Mr. George Ho), and the Azalea (1972) Endowment Fund.

References

 

DC FieldValueLanguage
dc.contributor.authorChiu, Ken_US
dc.contributor.authorZhou, Yen_US
dc.contributor.authorYeung, SCen_US
dc.contributor.authorLok, CKMen_US
dc.contributor.authorChan, OOCen_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorChiu, JFen_US
dc.date.accessioned2012-06-26T05:57:52Z-
dc.date.available2012-06-26T05:57:52Z-
dc.date.issued2010en_US
dc.identifier.citationJournal Of Cellular Biochemistry, 2010, v. 110 n. 2, p. 311-320en_US
dc.identifier.issn0730-2312en_US
dc.identifier.urihttp://hdl.handle.net/10722/149740-
dc.description.abstractWolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of-wolfberry extract-LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal βB2-crystallin. © 2010 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_US
dc.relation.ispartofJournal of Cellular Biochemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCrystallins - Metabolismen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshFemaleen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIntraocular Pressureen_US
dc.subject.meshLycium - Chemistryen_US
dc.subject.meshNeuroprotective Agents - Pharmacologyen_US
dc.subject.meshOcular Hypertension - Pathology - Physiopathologyen_US
dc.subject.meshPlant Extracts - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshRetinal Ganglion Cells - Drug Effects - Metabolismen_US
dc.subject.meshUp-Regulationen_US
dc.titleUp-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension modelen_US
dc.typeArticleen_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jcb.22539en_US
dc.identifier.pmid20336662en_US
dc.identifier.scopuseid_2-s2.0-77952066508en_US
dc.identifier.hkuros170567-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952066508&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume110en_US
dc.identifier.issue2en_US
dc.identifier.spage311en_US
dc.identifier.epage320en_US
dc.identifier.isiWOS:000277482700006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChiu, K=15076970500en_US
dc.identifier.scopusauthoridZhou, Y=7405366890en_US
dc.identifier.scopusauthoridYeung, SC=25923636600en_US
dc.identifier.scopusauthoridLok, CKM=36673388400en_US
dc.identifier.scopusauthoridChan, OOC=36672623700en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridChiu, JF=36672671600en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats