Article: A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean

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TitleA lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean
AuthorsFang, EF2
Lin, P2
Wong, JH2
Tsao, SW1
Ng, TB2
Issue Date2010
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
CitationJournal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229 [How to Cite?]
DOI: http://dx.doi.org/10.1021/jf903964u
AbstractLectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. © 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.
ISSN0021-8561
2011 Impact Factor: 2.823
2011 SCImago Journal Rankings: 0.144
DOIhttp://dx.doi.org/10.1021/jf903964u
ISI Accession Number IDWOS:000274530100025
Funding AgencyGrant Number
Chinese University of Hong Kong
Funding Information:

We thank Helen Hai-iing Liu, Yuan Zhao, and Wai-hung Lau from the Chinese University of Hong Kong For their kind support of this work.

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorFang, EF
dc.contributor.authorLin, P
dc.contributor.authorWong, JH
dc.contributor.authorTsao, SW
dc.contributor.authorNg, TB
dc.date.accessioned2012-06-26T05:57:47Z
dc.date.available2012-06-26T05:57:47Z
dc.date.issued2010
dc.description.abstractLectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. © 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229 [How to Cite?]
DOI: http://dx.doi.org/10.1021/jf903964u
dc.identifier.doihttp://dx.doi.org/10.1021/jf903964u
dc.identifier.epage2229
dc.identifier.hkuros182827
dc.identifier.isiWOS:000274530100025
Funding AgencyGrant Number
Chinese University of Hong Kong
Funding Information:

We thank Helen Hai-iing Liu, Yuan Zhao, and Wai-hung Lau from the Chinese University of Hong Kong For their kind support of this work.

dc.identifier.issn0021-8561
2011 Impact Factor: 2.823
2011 SCImago Journal Rankings: 0.144
dc.identifier.issue4
dc.identifier.pmid20095617
dc.identifier.scopuseid_2-s2.0-77249112249
dc.identifier.spage2221
dc.identifier.urihttp://hdl.handle.net/10722/149733
dc.identifier.volume58
dc.languageeng
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Agricultural and Food Chemistry
dc.relation.referencesReferences in Scopus
dc.subject.meshAmino Acid Sequence
dc.subject.meshAnimals
dc.subject.meshCell Division - Drug Effects
dc.subject.meshCell Line, Tumor
dc.subject.meshEnzyme Inhibitors - Chemistry - Isolation & Purification - Pharmacology
dc.subject.meshErythrocytes - Drug Effects - Physiology
dc.subject.meshFabaceae - Chemistry
dc.subject.meshHiv Reverse Transcriptase - Isolation & Purification
dc.subject.meshHiv-1 - Drug Effects - Enzymology
dc.subject.meshHemagglutination - Drug Effects
dc.subject.meshHumans
dc.subject.meshLectins - Isolation & Purification - Pharmacology
dc.subject.meshMice
dc.subject.meshMolecular Sequence Data
dc.subject.meshNasopharyngeal Neoplasms
dc.subject.meshNitric Oxide Synthase Type Ii - Drug Effects - Genetics
dc.subject.meshPhaseolus - Chemistry
dc.subject.meshRna, Messenger - Genetics
dc.subject.meshRabbits
dc.subject.meshRats
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshSeasons
dc.titleA lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Chinese University of Hong Kong