File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean
  • Basic View
  • Metadata View
  • XML View
TitleA lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean
 
AuthorsFang, EF2
Lin, P2
Wong, JH2
Tsao, SW1
Ng, TB2
 
Issue Date2010
 
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
 
CitationJournal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229 [How to Cite?]
DOI: http://dx.doi.org/10.1021/jf903964u
 
AbstractLectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. © 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.
 
ISSN0021-8561
2012 Impact Factor: 2.906
2012 SCImago Journal Rankings: 1.248
 
DOIhttp://dx.doi.org/10.1021/jf903964u
 
ISI Accession Number IDWOS:000274530100025
Funding AgencyGrant Number
Chinese University of Hong Kong
Funding Information:

We thank Helen Hai-iing Liu, Yuan Zhao, and Wai-hung Lau from the Chinese University of Hong Kong For their kind support of this work.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorFang, EF
 
dc.contributor.authorLin, P
 
dc.contributor.authorWong, JH
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorNg, TB
 
dc.date.accessioned2012-06-26T05:57:47Z
 
dc.date.available2012-06-26T05:57:47Z
 
dc.date.issued2010
 
dc.description.abstractLectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. © 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229 [How to Cite?]
DOI: http://dx.doi.org/10.1021/jf903964u
 
dc.identifier.doihttp://dx.doi.org/10.1021/jf903964u
 
dc.identifier.epage2229
 
dc.identifier.hkuros182827
 
dc.identifier.isiWOS:000274530100025
Funding AgencyGrant Number
Chinese University of Hong Kong
Funding Information:

We thank Helen Hai-iing Liu, Yuan Zhao, and Wai-hung Lau from the Chinese University of Hong Kong For their kind support of this work.

 
dc.identifier.issn0021-8561
2012 Impact Factor: 2.906
2012 SCImago Journal Rankings: 1.248
 
dc.identifier.issue4
 
dc.identifier.pmid20095617
 
dc.identifier.scopuseid_2-s2.0-77249112249
 
dc.identifier.spage2221
 
dc.identifier.urihttp://hdl.handle.net/10722/149733
 
dc.identifier.volume58
 
dc.languageeng
 
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Agricultural and Food Chemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAmino Acid Sequence
 
dc.subject.meshAnimals
 
dc.subject.meshCell Division - Drug Effects
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshEnzyme Inhibitors - Chemistry - Isolation & Purification - Pharmacology
 
dc.subject.meshErythrocytes - Drug Effects - Physiology
 
dc.subject.meshFabaceae - Chemistry
 
dc.subject.meshHiv Reverse Transcriptase - Isolation & Purification
 
dc.subject.meshHiv-1 - Drug Effects - Enzymology
 
dc.subject.meshHemagglutination - Drug Effects
 
dc.subject.meshHumans
 
dc.subject.meshLectins - Isolation & Purification - Pharmacology
 
dc.subject.meshMice
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshNasopharyngeal Neoplasms
 
dc.subject.meshNitric Oxide Synthase Type Ii - Drug Effects - Genetics
 
dc.subject.meshPhaseolus - Chemistry
 
dc.subject.meshRna, Messenger - Genetics
 
dc.subject.meshRabbits
 
dc.subject.meshRats
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshSeasons
 
dc.titleA lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Fang, EF</contributor.author>
<contributor.author>Lin, P</contributor.author>
<contributor.author>Wong, JH</contributor.author>
<contributor.author>Tsao, SW</contributor.author>
<contributor.author>Ng, TB</contributor.author>
<date.accessioned>2012-06-26T05:57:47Z</date.accessioned>
<date.available>2012-06-26T05:57:47Z</date.available>
<date.issued>2010</date.issued>
<identifier.citation>Journal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229</identifier.citation>
<identifier.issn>0021-8561</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/149733</identifier.uri>
<description.abstract>Lectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. &#169; 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.</description.abstract>
<language>eng</language>
<publisher>American Chemical Society. The Journal&apos;s web site is located at http://pubs.acs.org/journal/jafcau</publisher>
<relation.ispartof>Journal of Agricultural and Food Chemistry</relation.ispartof>
<subject.mesh>Amino Acid Sequence</subject.mesh>
<subject.mesh>Animals</subject.mesh>
<subject.mesh>Cell Division - Drug Effects</subject.mesh>
<subject.mesh>Cell Line, Tumor</subject.mesh>
<subject.mesh>Enzyme Inhibitors - Chemistry - Isolation &amp; Purification - Pharmacology</subject.mesh>
<subject.mesh>Erythrocytes - Drug Effects - Physiology</subject.mesh>
<subject.mesh>Fabaceae - Chemistry</subject.mesh>
<subject.mesh>Hiv Reverse Transcriptase - Isolation &amp; Purification</subject.mesh>
<subject.mesh>Hiv-1 - Drug Effects - Enzymology</subject.mesh>
<subject.mesh>Hemagglutination - Drug Effects</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Lectins - Isolation &amp; Purification - Pharmacology</subject.mesh>
<subject.mesh>Mice</subject.mesh>
<subject.mesh>Molecular Sequence Data</subject.mesh>
<subject.mesh>Nasopharyngeal Neoplasms</subject.mesh>
<subject.mesh>Nitric Oxide Synthase Type Ii - Drug Effects - Genetics</subject.mesh>
<subject.mesh>Phaseolus - Chemistry</subject.mesh>
<subject.mesh>Rna, Messenger - Genetics</subject.mesh>
<subject.mesh>Rabbits</subject.mesh>
<subject.mesh>Rats</subject.mesh>
<subject.mesh>Reverse Transcriptase Polymerase Chain Reaction</subject.mesh>
<subject.mesh>Seasons</subject.mesh>
<title>A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean</title>
<type>Article</type>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1021/jf903964u</identifier.doi>
<identifier.pmid>20095617</identifier.pmid>
<identifier.scopus>eid_2-s2.0-77249112249</identifier.scopus>
<identifier.hkuros>182827</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-77249112249&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>58</identifier.volume>
<identifier.issue>4</identifier.issue>
<identifier.spage>2221</identifier.spage>
<identifier.epage>2229</identifier.epage>
<identifier.isi>WOS:000274530100025</identifier.isi>
<publisher.place>United States</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Chinese University of Hong Kong