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Article: Induction of c-Jun phosphorylation in spinal motoneurons in neonatal and adult rats following axonal injury
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TitleInduction of c-Jun phosphorylation in spinal motoneurons in neonatal and adult rats following axonal injury
 
AuthorsYuan, Q1 4
Hu, B1
Wu, Y2
Chu, TH1
Su, H1
Zhang, W1
So, KF1 3
Lin, Z4
Wu, W1 3
 
Issue Date2010
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
 
CitationBrain Research, 2010, v. 1320, p. 7-15 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.brainres.2010.01.038
 
AbstractThis study aims to address if phosphorylation of the transcription factor c-Jun is associated with lesion-induced death of spinal motoneurons, and if this cellular response is modulated by glial-cell-line-derived neurotrophic factor (GDNF). We found that after both distal axotomy and root avulsion, spinal motoneurons in neonatal rats expressed phosphorylated c-Jun (p-c-Jun) and almost all injured motoneurons in these animals died. Similarly, root avulsion in adult rats also induced p-c-Jun expression that preceded the loss of motoneurons. In contrast, neither motoneuron death nor p-c-Jun induction was found after distal axotomy of spinal nerves in adult rats. Application of GDNF after distal axotomy in the neonatal model prevented motoneuron death but did not alter the expression of p-c-Jun in the surviving motoneurons. We conclude that c-Jun phosphorylation correlates with the cellular events leading to motoneuron death and that its expression cannot be modulated by GDNF. We further showed that expression of p-c-Jun was not correlated with the expression of growth-associated protein-43 (GAP-43), whose expression was closely correlated both temporally and spatially with periods of axonal outgrowth, suggesting that p-c-Jun may not be related with axonal regeneration of injured motoneurons. © 2010 Elsevier B.V. All rights reserved.
 
ISSN0006-8993
2013 Impact Factor: 2.828
2013 SCImago Journal Rankings: 1.572
 
DOIhttp://dx.doi.org/10.1016/j.brainres.2010.01.038
 
ISI Accession Number IDWOS:000275451000002
Funding AgencyGrant Number
HKU Spinal Cord Injury Foundation
University of Hong Kong
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by HKU Spinal Cord Injury Foundation and grants from the University of Hong Kong and Hong Kong Research Grants Council (RGC).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYuan, Q
 
dc.contributor.authorHu, B
 
dc.contributor.authorWu, Y
 
dc.contributor.authorChu, TH
 
dc.contributor.authorSu, H
 
dc.contributor.authorZhang, W
 
dc.contributor.authorSo, KF
 
dc.contributor.authorLin, Z
 
dc.contributor.authorWu, W
 
dc.date.accessioned2012-06-26T05:57:46Z
 
dc.date.available2012-06-26T05:57:46Z
 
dc.date.issued2010
 
dc.description.abstractThis study aims to address if phosphorylation of the transcription factor c-Jun is associated with lesion-induced death of spinal motoneurons, and if this cellular response is modulated by glial-cell-line-derived neurotrophic factor (GDNF). We found that after both distal axotomy and root avulsion, spinal motoneurons in neonatal rats expressed phosphorylated c-Jun (p-c-Jun) and almost all injured motoneurons in these animals died. Similarly, root avulsion in adult rats also induced p-c-Jun expression that preceded the loss of motoneurons. In contrast, neither motoneuron death nor p-c-Jun induction was found after distal axotomy of spinal nerves in adult rats. Application of GDNF after distal axotomy in the neonatal model prevented motoneuron death but did not alter the expression of p-c-Jun in the surviving motoneurons. We conclude that c-Jun phosphorylation correlates with the cellular events leading to motoneuron death and that its expression cannot be modulated by GDNF. We further showed that expression of p-c-Jun was not correlated with the expression of growth-associated protein-43 (GAP-43), whose expression was closely correlated both temporally and spatially with periods of axonal outgrowth, suggesting that p-c-Jun may not be related with axonal regeneration of injured motoneurons. © 2010 Elsevier B.V. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationBrain Research, 2010, v. 1320, p. 7-15 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.brainres.2010.01.038
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.brainres.2010.01.038
 
dc.identifier.epage15
 
dc.identifier.hkuros170571
 
dc.identifier.isiWOS:000275451000002
Funding AgencyGrant Number
HKU Spinal Cord Injury Foundation
University of Hong Kong
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by HKU Spinal Cord Injury Foundation and grants from the University of Hong Kong and Hong Kong Research Grants Council (RGC).

 
dc.identifier.issn0006-8993
2013 Impact Factor: 2.828
2013 SCImago Journal Rankings: 1.572
 
dc.identifier.pmid20096669
 
dc.identifier.scopuseid_2-s2.0-77049119905
 
dc.identifier.spage7
 
dc.identifier.urihttp://hdl.handle.net/10722/149732
 
dc.identifier.volume1320
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofBrain Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAging - Metabolism
 
dc.subject.meshAnimals
 
dc.subject.meshAnimals, Newborn
 
dc.subject.meshAxons - Metabolism
 
dc.subject.meshCell Count
 
dc.subject.meshCell Death - Physiology
 
dc.subject.meshCell Survival - Physiology
 
dc.subject.meshDisease Models, Animal
 
dc.subject.meshFemale
 
dc.subject.meshGap-43 Protein - Metabolism
 
dc.subject.meshGlial Cell Line-Derived Neurotrophic Factor - Metabolism
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshMotor Neurons - Metabolism
 
dc.subject.meshNerve Regeneration - Physiology
 
dc.subject.meshPhosphorylation
 
dc.subject.meshProto-Oncogene Proteins C-Jun - Metabolism
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshSpinal Cord - Metabolism
 
dc.subject.meshSpinal Nerves - Injuries - Metabolism
 
dc.titleInduction of c-Jun phosphorylation in spinal motoneurons in neonatal and adult rats following axonal injury
 
dc.typeArticle
 
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<contributor.author>Su, H</contributor.author>
<contributor.author>Zhang, W</contributor.author>
<contributor.author>So, KF</contributor.author>
<contributor.author>Lin, Z</contributor.author>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. UCL Institute of Child Health
  3. Jinan University
  4. Chinese University of Hong Kong