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Article: Effect of sonic hedgehog on the proliferation and differentiation of neural stem cells

TitleEffect of sonic hedgehog on the proliferation and differentiation of neural stem cells
Authors
KeywordsDifferentiation
Immunohistochemistry
Neural Stem Cell
Proliferation
Rat
Retinoic Acid
Sonic Hedgehog
Issue Date2009
Citation
Acta Anatomica Sinica, 2009, v. 40 n. 2, p. 211-214 How to Cite?
AbstractObjective: To explore the effect of sonic hedgehog on the proliferation and differentiation of neural stem cells (NSCs). Methods: NSCs isolated from the cortex and hippocampus of rat embryos, were divided into SHH group, RA group and PBS group, which were treated with sonic hedgehog (SHH), retinoic acid (RA) or PBS condition medium respectively. Effect of these treatments on the proliferation and differentiation of the NSCs were detected by immunocytochemistry. Results: NSCs could grow quickly and form neurospheres in culture. The cells in the neurospheres were positive for nestin which is one of the NSC markers. After the medium was supplemented with BrdU for 3h, the percentage of BrdU positive cells in the SHH group was significandy higher than that of the RA group and PBS group. However, there was no obvious difference between RA group and PBS group. After the NSCs were cultured in differentiating condition medium for 7 days, the percentages of βIII-tubulin and Rip positive cells in the SHH treated group were significantly higher than that in the RA and PBS treated groups. Conclusions: SHH could promote NSC proliferation and differentiation into neurons and oligodendrocytes.
Persistent Identifierhttp://hdl.handle.net/10722/149713
ISSN
2015 SCImago Journal Rankings: 0.121
References

 

DC FieldValueLanguage
dc.contributor.authorZou, Len_US
dc.contributor.authorGuo, JSen_US
dc.contributor.authorDai, Xen_US
dc.contributor.authorDai, JXen_US
dc.contributor.authorDong, WRen_US
dc.contributor.authorAn, Jen_US
dc.contributor.authorWu, WTen_US
dc.date.accessioned2012-06-26T05:57:29Z-
dc.date.available2012-06-26T05:57:29Z-
dc.date.issued2009en_US
dc.identifier.citationActa Anatomica Sinica, 2009, v. 40 n. 2, p. 211-214en_US
dc.identifier.issn0529-1356en_US
dc.identifier.urihttp://hdl.handle.net/10722/149713-
dc.description.abstractObjective: To explore the effect of sonic hedgehog on the proliferation and differentiation of neural stem cells (NSCs). Methods: NSCs isolated from the cortex and hippocampus of rat embryos, were divided into SHH group, RA group and PBS group, which were treated with sonic hedgehog (SHH), retinoic acid (RA) or PBS condition medium respectively. Effect of these treatments on the proliferation and differentiation of the NSCs were detected by immunocytochemistry. Results: NSCs could grow quickly and form neurospheres in culture. The cells in the neurospheres were positive for nestin which is one of the NSC markers. After the medium was supplemented with BrdU for 3h, the percentage of BrdU positive cells in the SHH group was significandy higher than that of the RA group and PBS group. However, there was no obvious difference between RA group and PBS group. After the NSCs were cultured in differentiating condition medium for 7 days, the percentages of βIII-tubulin and Rip positive cells in the SHH treated group were significantly higher than that in the RA and PBS treated groups. Conclusions: SHH could promote NSC proliferation and differentiation into neurons and oligodendrocytes.en_US
dc.languageengen_US
dc.relation.ispartofActa Anatomica Sinicaen_US
dc.subjectDifferentiationen_US
dc.subjectImmunohistochemistryen_US
dc.subjectNeural Stem Cellen_US
dc.subjectProliferationen_US
dc.subjectRaten_US
dc.subjectRetinoic Aciden_US
dc.subjectSonic Hedgehogen_US
dc.titleEffect of sonic hedgehog on the proliferation and differentiation of neural stem cellsen_US
dc.typeArticleen_US
dc.identifier.emailWu, WT:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, WT=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3969/j.issn.0529-1356.2009.02.008en_US
dc.identifier.scopuseid_2-s2.0-66749192819en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66749192819&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue2en_US
dc.identifier.spage211en_US
dc.identifier.epage214en_US
dc.identifier.scopusauthoridZou, L=27268086600en_US
dc.identifier.scopusauthoridGuo, JS=7404488603en_US
dc.identifier.scopusauthoridDai, X=35285685000en_US
dc.identifier.scopusauthoridDai, JX=7401679121en_US
dc.identifier.scopusauthoridDong, WR=15032660300en_US
dc.identifier.scopusauthoridAn, J=55187515800en_US
dc.identifier.scopusauthoridWu, WT=7407081122en_US

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