File Download
  • No File Attached
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Lithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor
  • Basic View
  • Metadata View
  • XML View
TitleLithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor
 
AuthorsSu, H1
Zhang, W1
Guo, J1
Guo, A1
Yuan, Q1
Wu, W1 1 1 1
 
Issue Date2009
 
CitationJournal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
 
AbstractThis study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. © 2009 International Society for Neurochemistry.
 
ISSN0022-3042
2012 Impact Factor: 3.973
2012 SCImago Journal Rankings: 1.754
 
DOIhttp://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
 
ISI Accession Number IDWOS:000263696300006
Funding AgencyGrant Number
The Spinal Cord Injury Foundation of the University of Hong Kong
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by grants from The Spinal Cord Injury Foundation of the University of Hong Kong and Hong Kong Research Grants Council (RGC).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSu, H
 
dc.contributor.authorZhang, W
 
dc.contributor.authorGuo, J
 
dc.contributor.authorGuo, A
 
dc.contributor.authorYuan, Q
 
dc.contributor.authorWu, W
 
dc.date.accessioned2012-06-26T05:57:28Z
 
dc.date.available2012-06-26T05:57:28Z
 
dc.date.issued2009
 
dc.description.abstractThis study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. © 2009 International Society for Neurochemistry.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
 
dc.identifier.citeulike4098191
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
 
dc.identifier.epage1398
 
dc.identifier.hkuros163970
 
dc.identifier.isiWOS:000263696300006
Funding AgencyGrant Number
The Spinal Cord Injury Foundation of the University of Hong Kong
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by grants from The Spinal Cord Injury Foundation of the University of Hong Kong and Hong Kong Research Grants Council (RGC).

 
dc.identifier.issn0022-3042
2012 Impact Factor: 3.973
2012 SCImago Journal Rankings: 1.754
 
dc.identifier.issue6
 
dc.identifier.pmid19183259
 
dc.identifier.scopuseid_2-s2.0-61349141269
 
dc.identifier.spage1385
 
dc.identifier.urihttp://hdl.handle.net/10722/149711
 
dc.identifier.volume108
 
dc.languageeng
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Neurochemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnalysis Of Variance
 
dc.subject.meshAnimals
 
dc.subject.meshAnimals, Genetically Modified
 
dc.subject.meshAnimals, Newborn
 
dc.subject.meshAnterior Horn Cells - Cytology
 
dc.subject.meshAntibodies - Pharmacology
 
dc.subject.meshBrain-Derived Neurotrophic Factor - Immunology - Metabolism
 
dc.subject.meshBromodeoxyuridine - Metabolism
 
dc.subject.meshCell Differentiation - Drug Effects
 
dc.subject.meshCell Transplantation - Methods
 
dc.subject.meshCells, Cultured
 
dc.subject.meshEmbryo, Mammalian
 
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methods
 
dc.subject.meshFemale
 
dc.subject.meshGreen Fluorescent Proteins - Genetics
 
dc.subject.meshLithium Chloride - Pharmacology
 
dc.subject.meshNerve Tissue Proteins - Metabolism
 
dc.subject.meshNeurogenesis - Drug Effects
 
dc.subject.meshNeurons - Drug Effects
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshSpinal Nerve Roots - Cytology - Metabolism - Surgery
 
dc.subject.meshStem Cells - Drug Effects
 
dc.titleLithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Su, H</contributor.author>
<contributor.author>Zhang, W</contributor.author>
<contributor.author>Guo, J</contributor.author>
<contributor.author>Guo, A</contributor.author>
<contributor.author>Yuan, Q</contributor.author>
<contributor.author>Wu, W</contributor.author>
<date.accessioned>2012-06-26T05:57:28Z</date.accessioned>
<date.available>2012-06-26T05:57:28Z</date.available>
<date.issued>2009</date.issued>
<identifier.citation>Journal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398</identifier.citation>
<identifier.issn>0022-3042</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/149711</identifier.uri>
<description.abstract>This study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. &#169; 2009 International Society for Neurochemistry.</description.abstract>
<language>eng</language>
<relation.ispartof>Journal of Neurochemistry</relation.ispartof>
<subject.mesh>Analysis Of Variance</subject.mesh>
<subject.mesh>Animals</subject.mesh>
<subject.mesh>Animals, Genetically Modified</subject.mesh>
<subject.mesh>Animals, Newborn</subject.mesh>
<subject.mesh>Anterior Horn Cells - Cytology</subject.mesh>
<subject.mesh>Antibodies - Pharmacology</subject.mesh>
<subject.mesh>Brain-Derived Neurotrophic Factor - Immunology - Metabolism</subject.mesh>
<subject.mesh>Bromodeoxyuridine - Metabolism</subject.mesh>
<subject.mesh>Cell Differentiation - Drug Effects</subject.mesh>
<subject.mesh>Cell Transplantation - Methods</subject.mesh>
<subject.mesh>Cells, Cultured</subject.mesh>
<subject.mesh>Embryo, Mammalian</subject.mesh>
<subject.mesh>Enzyme-Linked Immunosorbent Assay - Methods</subject.mesh>
<subject.mesh>Female</subject.mesh>
<subject.mesh>Green Fluorescent Proteins - Genetics</subject.mesh>
<subject.mesh>Lithium Chloride - Pharmacology</subject.mesh>
<subject.mesh>Nerve Tissue Proteins - Metabolism</subject.mesh>
<subject.mesh>Neurogenesis - Drug Effects</subject.mesh>
<subject.mesh>Neurons - Drug Effects</subject.mesh>
<subject.mesh>Rats</subject.mesh>
<subject.mesh>Rats, Sprague-Dawley</subject.mesh>
<subject.mesh>Spinal Nerve Roots - Cytology - Metabolism - Surgery</subject.mesh>
<subject.mesh>Stem Cells - Drug Effects</subject.mesh>
<title>Lithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor</title>
<type>Article</type>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.doi>10.1111/j.1471-4159.2009.05902.x</identifier.doi>
<identifier.pmid>19183259</identifier.pmid>
<identifier.scopus>eid_2-s2.0-61349141269</identifier.scopus>
<identifier.hkuros>163970</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-61349141269&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>108</identifier.volume>
<identifier.issue>6</identifier.issue>
<identifier.spage>1385</identifier.spage>
<identifier.epage>1398</identifier.epage>
<identifier.isi>WOS:000263696300006</identifier.isi>
<publisher.place>United Kingdom</publisher.place>
<identifier.citeulike>4098191</identifier.citeulike>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine