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Article: Lithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor

TitleLithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor
Authors
Issue Date2009
Citation
Journal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398 How to Cite?
Abstract
This study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. © 2009 International Society for Neurochemistry.
Persistent Identifierhttp://hdl.handle.net/10722/149711
ISSN
2013 Impact Factor: 4.244
ISI Accession Number ID
Funding AgencyGrant Number
The Spinal Cord Injury Foundation of the University of Hong Kong
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by grants from The Spinal Cord Injury Foundation of the University of Hong Kong and Hong Kong Research Grants Council (RGC).

References

 

DC FieldValueLanguage
dc.contributor.authorSu, Hen_US
dc.contributor.authorZhang, Wen_US
dc.contributor.authorGuo, Jen_US
dc.contributor.authorGuo, Aen_US
dc.contributor.authorYuan, Qen_US
dc.contributor.authorWu, Wen_US
dc.date.accessioned2012-06-26T05:57:28Z-
dc.date.available2012-06-26T05:57:28Z-
dc.date.issued2009en_US
dc.identifier.citationJournal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398en_US
dc.identifier.issn0022-3042en_US
dc.identifier.urihttp://hdl.handle.net/10722/149711-
dc.description.abstractThis study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. © 2009 International Society for Neurochemistry.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Neurochemistryen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Genetically Modifieden_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshAnterior Horn Cells - Cytologyen_US
dc.subject.meshAntibodies - Pharmacologyen_US
dc.subject.meshBrain-Derived Neurotrophic Factor - Immunology - Metabolismen_US
dc.subject.meshBromodeoxyuridine - Metabolismen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshCell Transplantation - Methodsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEmbryo, Mammalianen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methodsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGreen Fluorescent Proteins - Geneticsen_US
dc.subject.meshLithium Chloride - Pharmacologyen_US
dc.subject.meshNerve Tissue Proteins - Metabolismen_US
dc.subject.meshNeurogenesis - Drug Effectsen_US
dc.subject.meshNeurons - Drug Effectsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSpinal Nerve Roots - Cytology - Metabolism - Surgeryen_US
dc.subject.meshStem Cells - Drug Effectsen_US
dc.titleLithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factoren_US
dc.typeArticleen_US
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1471-4159.2009.05902.xen_US
dc.identifier.pmid19183259en_US
dc.identifier.scopuseid_2-s2.0-61349141269en_US
dc.identifier.hkuros163970-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61349141269&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume108en_US
dc.identifier.issue6en_US
dc.identifier.spage1385en_US
dc.identifier.epage1398en_US
dc.identifier.isiWOS:000263696300006-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridSu, H=16317750200en_US
dc.identifier.scopusauthoridZhang, W=9044147100en_US
dc.identifier.scopusauthoridGuo, J=7404488603en_US
dc.identifier.scopusauthoridGuo, A=55137882000en_US
dc.identifier.scopusauthoridYuan, Q=7202814773en_US
dc.identifier.scopusauthoridWu, W=7407081122en_US
dc.identifier.citeulike4098191-

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