Article: Lithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor

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TitleLithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor
AuthorsSu, H1
Zhang, W1
Guo, J1
Guo, A1
Yuan, Q1
Wu, W1
Issue Date2009
CitationJournal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
AbstractThis study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. © 2009 International Society for Neurochemistry.
ISSN0022-3042
2011 Impact Factor: 4.061
2011 SCImago Journal Rankings: 0.391
DOIhttp://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorSu, H
dc.contributor.authorZhang, W
dc.contributor.authorGuo, J
dc.contributor.authorGuo, A
dc.contributor.authorYuan, Q
dc.contributor.authorWu, W
dc.date.accessioned2012-06-26T05:57:28Z
dc.date.available2012-06-26T05:57:28Z
dc.date.issued2009
dc.description.abstractThis study was undertaken to elucidate the molecular mechanisms by which lithium regulates the development of spinal cord-derived neural progenitor cells (NPCs) in vitro and after transplanted in vivo. Our results show that lithium at the therapeutic concentration significantly increases the proliferation and neuronal differentiation of NPCs in vitro. Specific ELISAs, western blotting, and quantitative real-time RT-PCR assays demonstrate that lithium treatment significantly elevates the expression and production of brain-derived neurotrophic factor (BDNF) by NPCs in culture. Application of a BDNF neutralizing antibody in culture leads to a marked reduction in the neurogenesis of lithium-treated NPCs to the control level. However, it shows no effects on the proliferation of lithium-treated NPCs. These findings suggest that the BDNF pathway is possibly involved in the supportive role of lithium in inducing NPC neurogenesis but not proliferation. This study also provides evidence that lithium is able to elevate the neuronal generation and BDNF production of NPCs after transplantation into the adult rat ventral horn with motoneuron degeneration because of spinal root avulsion, which highlights the therapeutic potential of lithium in cell replacement strategies for spinal cord injury because of its ability to promote neuronal differentiation and BDNF production of grafted NPCs in the injured spinal cord. © 2009 International Society for Neurochemistry.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Neurochemistry, 2009, v. 108 n. 6, p. 1385-1398 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
dc.identifier.citeulike4098191
dc.identifier.doihttp://dx.doi.org/10.1111/j.1471-4159.2009.05902.x
dc.identifier.epage1398
dc.identifier.hkuros163970
dc.identifier.isiWOS:000263696300006
Funding AgencyGrant Number
The Spinal Cord Injury Foundation of the University of Hong Kong
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by grants from The Spinal Cord Injury Foundation of the University of Hong Kong and Hong Kong Research Grants Council (RGC).

dc.identifier.issn0022-3042
2011 Impact Factor: 4.061
2011 SCImago Journal Rankings: 0.391
dc.identifier.issue6
dc.identifier.pmid19183259
dc.identifier.scopuseid_2-s2.0-61349141269
dc.identifier.spage1385
dc.identifier.urihttp://hdl.handle.net/10722/149711
dc.identifier.volume108
dc.languageeng
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of Neurochemistry
dc.relation.referencesReferences in Scopus
dc.subject.meshAnalysis Of Variance
dc.subject.meshAnimals
dc.subject.meshAnimals, Genetically Modified
dc.subject.meshAnimals, Newborn
dc.subject.meshAnterior Horn Cells - Cytology
dc.subject.meshAntibodies - Pharmacology
dc.subject.meshBrain-Derived Neurotrophic Factor - Immunology - Metabolism
dc.subject.meshBromodeoxyuridine - Metabolism
dc.subject.meshCell Differentiation - Drug Effects
dc.subject.meshCell Transplantation - Methods
dc.subject.meshCells, Cultured
dc.subject.meshEmbryo, Mammalian
dc.subject.meshEnzyme-Linked Immunosorbent Assay - Methods
dc.subject.meshFemale
dc.subject.meshGreen Fluorescent Proteins - Genetics
dc.subject.meshLithium Chloride - Pharmacology
dc.subject.meshNerve Tissue Proteins - Metabolism
dc.subject.meshNeurogenesis - Drug Effects
dc.subject.meshNeurons - Drug Effects
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshSpinal Nerve Roots - Cytology - Metabolism - Surgery
dc.subject.meshStem Cells - Drug Effects
dc.titleLithium enhances the neuronal differentiation of neural progenitor cells in vitro and after transplantation into the avulsed ventral horn of adult rats through the secretion of brain-derived neurotrophic factor
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine