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Article: A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
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TitleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
 
AuthorsKoo, CK3
Wong, KL3
Man, CWY1
Lam, YW3
So, LKY3
Tam, HL2
Tsao, SW1
Cheah, KW2
Lau, KC3
Yang, YY4
Chen, JC4
Lam, MHW3
 
Issue Date2009
 
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
 
CitationInorganic Chemistry, 2009, v. 48 n. 3, p. 872-878 [How to Cite?]
DOI: http://dx.doi.org/10.1021/ic801261h
 
AbstractThe cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
 
ISSN0020-1669
2012 Impact Factor: 4.593
2012 SCImago Journal Rankings: 1.778
 
DOIhttp://dx.doi.org/10.1021/ic801261h
 
ISI Accession Number IDWOS:000262782900021
Funding AgencyGrant Number
Hong Kong Research Grants Council
Hong Kong Baptist University
City University of Hong Kong
Funding Information:

The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKoo, CK
 
dc.contributor.authorWong, KL
 
dc.contributor.authorMan, CWY
 
dc.contributor.authorLam, YW
 
dc.contributor.authorSo, LKY
 
dc.contributor.authorTam, HL
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorCheah, KW
 
dc.contributor.authorLau, KC
 
dc.contributor.authorYang, YY
 
dc.contributor.authorChen, JC
 
dc.contributor.authorLam, MHW
 
dc.date.accessioned2012-06-26T05:57:28Z
 
dc.date.available2012-06-26T05:57:28Z
 
dc.date.issued2009
 
dc.description.abstractThe cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationInorganic Chemistry, 2009, v. 48 n. 3, p. 872-878 [How to Cite?]
DOI: http://dx.doi.org/10.1021/ic801261h
 
dc.identifier.doihttp://dx.doi.org/10.1021/ic801261h
 
dc.identifier.epage878
 
dc.identifier.hkuros165259
 
dc.identifier.isiWOS:000262782900021
Funding AgencyGrant Number
Hong Kong Research Grants Council
Hong Kong Baptist University
City University of Hong Kong
Funding Information:

The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong.

 
dc.identifier.issn0020-1669
2012 Impact Factor: 4.593
2012 SCImago Journal Rankings: 1.778
 
dc.identifier.issue3
 
dc.identifier.pmid19128152
 
dc.identifier.scopuseid_2-s2.0-60849111428
 
dc.identifier.spage872
 
dc.identifier.urihttp://hdl.handle.net/10722/149710
 
dc.identifier.volume48
 
dc.languageeng
 
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
 
dc.publisher.placeUnited States
 
dc.relation.ispartofInorganic Chemistry
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCell Line
 
dc.subject.meshCell Survival - Drug Effects
 
dc.subject.meshCyclization
 
dc.subject.meshHumans
 
dc.subject.meshMice
 
dc.subject.meshMolecular Structure
 
dc.subject.meshPhotochemistry
 
dc.subject.meshPhotons
 
dc.subject.meshPlatinum Compounds - Chemistry - Toxicity
 
dc.subject.meshSpectrophotometry
 
dc.titleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Baptist University
  3. City University of Hong Kong
  4. Sun Yat-Sen University