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Article: A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

TitleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
Authors
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
Citation
Inorganic Chemistry, 2009, v. 48 n. 3, p. 872-878 How to Cite?
Abstract
The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/149710
ISSN
2013 Impact Factor: 4.794
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants Council
Hong Kong Baptist University
City University of Hong Kong
Funding Information:

The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorKoo, CKen_US
dc.contributor.authorWong, KLen_US
dc.contributor.authorMan, CWYen_US
dc.contributor.authorLam, YWen_US
dc.contributor.authorSo, LKYen_US
dc.contributor.authorTam, HLen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorCheah, KWen_US
dc.contributor.authorLau, KCen_US
dc.contributor.authorYang, YYen_US
dc.contributor.authorChen, JCen_US
dc.contributor.authorLam, MHWen_US
dc.date.accessioned2012-06-26T05:57:28Z-
dc.date.available2012-06-26T05:57:28Z-
dc.date.issued2009en_US
dc.identifier.citationInorganic Chemistry, 2009, v. 48 n. 3, p. 872-878en_US
dc.identifier.issn0020-1669en_US
dc.identifier.urihttp://hdl.handle.net/10722/149710-
dc.description.abstractThe cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/icen_US
dc.relation.ispartofInorganic Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCyclizationen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshPhotochemistryen_US
dc.subject.meshPhotonsen_US
dc.subject.meshPlatinum Compounds - Chemistry - Toxicityen_US
dc.subject.meshSpectrophotometryen_US
dc.titleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imagingen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ic801261hen_US
dc.identifier.pmid19128152en_US
dc.identifier.scopuseid_2-s2.0-60849111428en_US
dc.identifier.hkuros165259-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60849111428&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume48en_US
dc.identifier.issue3en_US
dc.identifier.spage872en_US
dc.identifier.epage878en_US
dc.identifier.isiWOS:000262782900021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKoo, CK=15756031600en_US
dc.identifier.scopusauthoridWong, KL=35239206000en_US
dc.identifier.scopusauthoridMan, CWY=7005722377en_US
dc.identifier.scopusauthoridLam, YW=7202563947en_US
dc.identifier.scopusauthoridSo, LKY=7007150474en_US
dc.identifier.scopusauthoridTam, HL=7101835048en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridCheah, KW=7102792922en_US
dc.identifier.scopusauthoridLau, KC=7401560253en_US
dc.identifier.scopusauthoridYang, YY=14526231600en_US
dc.identifier.scopusauthoridChen, JC=8678427800en_US
dc.identifier.scopusauthoridLam, MHW=7202630175en_US

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