Article: A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

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TitleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
AuthorsKoo, CK2
Wong, KL2
Man, CWY1
Lam, YW2
So, LKY2
Tam, HL3
Tsao, SW1
Cheah, KW3
Lau, KC2
Yang, YY4
Chen, JC4
Lam, MHW2
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
CitationInorganic Chemistry, 2009, v. 48 n. 3, p. 872-878 [How to Cite?]
DOI: http://dx.doi.org/10.1021/ic801261h
AbstractThe cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
ISSN0020-1669
2011 Impact Factor: 4.601
2011 SCImago Journal Rankings: 0.315
DOIhttp://dx.doi.org/10.1021/ic801261h
ISI Accession Number IDWOS:000262782900021
Funding AgencyGrant Number
Hong Kong Research Grants Council
Hong Kong Baptist University
City University of Hong Kong
Funding Information:

The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong.

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorKoo, CK
dc.contributor.authorWong, KL
dc.contributor.authorMan, CWY
dc.contributor.authorLam, YW
dc.contributor.authorSo, LKY
dc.contributor.authorTam, HL
dc.contributor.authorTsao, SW
dc.contributor.authorCheah, KW
dc.contributor.authorLau, KC
dc.contributor.authorYang, YY
dc.contributor.authorChen, JC
dc.contributor.authorLam, MHW
dc.date.accessioned2012-06-26T05:57:28Z
dc.date.available2012-06-26T05:57:28Z
dc.date.issued2009
dc.description.abstractThe cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationInorganic Chemistry, 2009, v. 48 n. 3, p. 872-878 [How to Cite?]
DOI: http://dx.doi.org/10.1021/ic801261h
dc.identifier.doihttp://dx.doi.org/10.1021/ic801261h
dc.identifier.epage878
dc.identifier.hkuros165259
dc.identifier.isiWOS:000262782900021
Funding AgencyGrant Number
Hong Kong Research Grants Council
Hong Kong Baptist University
City University of Hong Kong
Funding Information:

The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong.

dc.identifier.issn0020-1669
2011 Impact Factor: 4.601
2011 SCImago Journal Rankings: 0.315
dc.identifier.issue3
dc.identifier.pmid19128152
dc.identifier.scopuseid_2-s2.0-60849111428
dc.identifier.spage872
dc.identifier.urihttp://hdl.handle.net/10722/149710
dc.identifier.volume48
dc.languageeng
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
dc.publisher.placeUnited States
dc.relation.ispartofInorganic Chemistry
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshCell Line
dc.subject.meshCell Survival - Drug Effects
dc.subject.meshCyclization
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshMolecular Structure
dc.subject.meshPhotochemistry
dc.subject.meshPhotons
dc.subject.meshPlatinum Compounds - Chemistry - Toxicity
dc.subject.meshSpectrophotometry
dc.titleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong
  3. Hong Kong Baptist University
  4. Sun Yat-Sen University