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Article: A bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging

TitleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imaging
Authors
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ic
Citation
Inorganic Chemistry, 2009, v. 48 n. 3, p. 872-878 How to Cite?
Abstract
The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/149710
ISSN
2013 Impact Factor: 4.794
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants Council
Hong Kong Baptist University
City University of Hong Kong
Funding Information:

The authors acknowledge financial support from the Hong Kong Research Grants Council, City University of Hong Kong, and Hong Kong Baptist University. K.-L.W. acknowledges a Research Scholarship Enhancement Scheme from City University of Hong Kong.

References

 

Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Baptist University
  3. City University of Hong Kong
  4. Sun Yat-Sen University
DC FieldValueLanguage
dc.contributor.authorKoo, CKen_US
dc.contributor.authorWong, KLen_US
dc.contributor.authorMan, CWYen_US
dc.contributor.authorLam, YWen_US
dc.contributor.authorSo, LKYen_US
dc.contributor.authorTam, HLen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorCheah, KWen_US
dc.contributor.authorLau, KCen_US
dc.contributor.authorYang, YYen_US
dc.contributor.authorChen, JCen_US
dc.contributor.authorLam, MHWen_US
dc.date.accessioned2012-06-26T05:57:28Z-
dc.date.available2012-06-26T05:57:28Z-
dc.date.issued2009en_US
dc.identifier.citationInorganic Chemistry, 2009, v. 48 n. 3, p. 872-878en_US
dc.identifier.issn0020-1669en_US
dc.identifier.urihttp://hdl.handle.net/10722/149710-
dc.description.abstractThe cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing C phenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h. © 2009 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/icen_US
dc.relation.ispartofInorganic Chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshCyclizationen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Structureen_US
dc.subject.meshPhotochemistryen_US
dc.subject.meshPhotonsen_US
dc.subject.meshPlatinum Compounds - Chemistry - Toxicityen_US
dc.subject.meshSpectrophotometryen_US
dc.titleA bioaccumulative cyclometalated platinum(II) complex with two-photon-induced emission for live cell imagingen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ic801261hen_US
dc.identifier.pmid19128152en_US
dc.identifier.scopuseid_2-s2.0-60849111428en_US
dc.identifier.hkuros165259-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60849111428&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume48en_US
dc.identifier.issue3en_US
dc.identifier.spage872en_US
dc.identifier.epage878en_US
dc.identifier.isiWOS:000262782900021-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridKoo, CK=15756031600en_US
dc.identifier.scopusauthoridWong, KL=35239206000en_US
dc.identifier.scopusauthoridMan, CWY=7005722377en_US
dc.identifier.scopusauthoridLam, YW=7202563947en_US
dc.identifier.scopusauthoridSo, LKY=7007150474en_US
dc.identifier.scopusauthoridTam, HL=7101835048en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridCheah, KW=7102792922en_US
dc.identifier.scopusauthoridLau, KC=7401560253en_US
dc.identifier.scopusauthoridYang, YY=14526231600en_US
dc.identifier.scopusauthoridChen, JC=8678427800en_US
dc.identifier.scopusauthoridLam, MHW=7202630175en_US

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