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Article: p27 Kip1 promotes migration of metastatic hepatocellular carcinoma cells

Titlep27 Kip1 promotes migration of metastatic hepatocellular carcinoma cells
Authors
KeywordsCell migration
Hepatocellular carcinoma
p27
RhoA-GTP
Issue Date2008
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBI
Citation
Tumor Biology, 2008, v. 29 n. 4, p. 217-223 How to Cite?
AbstractBackground/Aim: p27 Kip1 (p27) is a member of the Cip/Kip family of cyclin/cyclin-dependent kinase inhibitors (CKIs), and its CKI-independent function regarding cell motility modulation has been discovered. However, it is controversial whether p27 promotes or inhibits cell migration. This study investigates the migration regulatory role of p27 in metastatic hepatocellular carcinoma (HCC) cells. Methods: RNA interference, RhoA-GTP pull-down assay, Western blots, immunostaining, transwell and wound-healing assays were used. Results: High levels of p27 and phosphorylated p27 (Ser10) were detected in metastatic HCC cells, MHCC97L and MHCC97H, when compared with nonmetastatic HCC cells, PLC and Hep3B. We hypothesized that p27 is responsible for metastasis-related migration in HCC cells and tested the hypothesis by using the p27 RNA interference approach. Increased RhoA activity was observed when p27 was knocked down in MHCC97L cells, which further led to stress fiber formation and decreased cell migration and wound healing. Moreover, high p27 and low stathmin expression of metastatic HCC cells indicated that migration inhibition by p27-stathmin interaction might not be the major regulatory pathway in metastatic HCC cells. Conclusion: p27 promotes cell migration in metastatic HCC cells through the regulation of RhoA activity. Copyright © 2008 S. Karger AG.
Persistent Identifierhttp://hdl.handle.net/10722/149703
ISSN
2015 Impact Factor: 2.926
2015 SCImago Journal Rankings: 0.989
ISI Accession Number ID
Funding AgencyGrant Number
HKU59002
SCY Research Foundation
Funding Information:

This work was supported by HKU research grant 59002 and SCY Research Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorWang, XQen_HK
dc.contributor.authorLui, ELHen_HK
dc.contributor.authorCai, Qen_HK
dc.contributor.authorChing, WYPen_HK
dc.contributor.authorLiu, KSYen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2012-06-26T05:57:21Z-
dc.date.available2012-06-26T05:57:21Z-
dc.date.issued2008en_HK
dc.identifier.citationTumor Biology, 2008, v. 29 n. 4, p. 217-223en_HK
dc.identifier.issn1010-4283en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149703-
dc.description.abstractBackground/Aim: p27 Kip1 (p27) is a member of the Cip/Kip family of cyclin/cyclin-dependent kinase inhibitors (CKIs), and its CKI-independent function regarding cell motility modulation has been discovered. However, it is controversial whether p27 promotes or inhibits cell migration. This study investigates the migration regulatory role of p27 in metastatic hepatocellular carcinoma (HCC) cells. Methods: RNA interference, RhoA-GTP pull-down assay, Western blots, immunostaining, transwell and wound-healing assays were used. Results: High levels of p27 and phosphorylated p27 (Ser10) were detected in metastatic HCC cells, MHCC97L and MHCC97H, when compared with nonmetastatic HCC cells, PLC and Hep3B. We hypothesized that p27 is responsible for metastasis-related migration in HCC cells and tested the hypothesis by using the p27 RNA interference approach. Increased RhoA activity was observed when p27 was knocked down in MHCC97L cells, which further led to stress fiber formation and decreased cell migration and wound healing. Moreover, high p27 and low stathmin expression of metastatic HCC cells indicated that migration inhibition by p27-stathmin interaction might not be the major regulatory pathway in metastatic HCC cells. Conclusion: p27 promotes cell migration in metastatic HCC cells through the regulation of RhoA activity. Copyright © 2008 S. Karger AG.en_HK
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/TBIen_HK
dc.relation.ispartofTumor Biologyen_HK
dc.subjectCell migrationen_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectp27en_HK
dc.subjectRhoA-GTPen_HK
dc.titlep27 Kip1 promotes migration of metastatic hepatocellular carcinoma cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, XQ: xqwang@hkucc.hku.hken_HK
dc.identifier.emailChing, WYP: ypching@hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityWang, XQ=rp00507en_HK
dc.identifier.authorityChing, WYP=rp00469en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1159/000152939en_HK
dc.identifier.pmid18781093-
dc.identifier.scopuseid_2-s2.0-51049085835en_HK
dc.identifier.hkuros152551-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51049085835&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue4en_HK
dc.identifier.spage217en_HK
dc.identifier.epage223en_HK
dc.identifier.isiWOS:000259892500002-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridWang, XQ=17343159900en_HK
dc.identifier.scopusauthoridLui, ELH=36865643400en_HK
dc.identifier.scopusauthoridCai, Q=25935876300en_HK
dc.identifier.scopusauthoridChing, WYP=7005431277en_HK
dc.identifier.scopusauthoridLiu, KSY=25031724200en_HK
dc.identifier.scopusauthoridPoon, RTP=7103097223en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK

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