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Article: Label-retaining cells of the bladder: Candidate urothelial stem cells

TitleLabel-retaining cells of the bladder: Candidate urothelial stem cells
Authors
KeywordsAdult Stem Cells
Epithelium
Rats
Issue Date2008
Citation
American Journal Of Physiology - Renal Physiology, 2008, v. 294 n. 6, p. F1415-F1421 How to Cite?
AbstractAdult tissue stem cells replicate infrequently, retaining DNA nucleotide label (BrdU) for much longer periods than mature, dividing cells in which the label is diluted during a chase period. Those "label-retaining cells" (LRCs) have been identified as the tissue stem cells in skin, cornea, intestine, and prostate. However, in the urinary tract uroepithelial stem cells have not yet been identified. In this study, BrdU administration identified urothelial LRCs in the rat bladder with 9% of the epithelial basal cells retaining BrdU label 1 yr after its administration. Markers for stem cells in other tissues, Bcl, p63, cytokeratin 14, and β1 integrin, were immunolocalized in the basal bladder epithelium in or near urothelial LRCs, but not uniquely limited to these cells. Flow cytometry demonstrated that urothelial LRCs were small, had low granularity, and were uniquely β4 integrin bright. Urothelium from long-term labeled bladders was cultured and LRCs were found to be significantly more clonogenic and proliferative, characteristics of stem cells, than unlabeled urothelial cells. Thus, this work demonstrates that LRCs in the bladder localize to the basal layer, are small, low granularity, uniquely β4 integrin rich, slowly cycling and demonstrate superior clonogenic and proliferative ability compared with unlabeled epithelial cells. We propose that LRCs represent putative urothelial stem cells. Copyright © 2008 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/149701
ISSN
2008 Impact Factor: 3.89
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKurzrock, EAen_US
dc.contributor.authorLieu, DKen_US
dc.contributor.authorDegraffenried, LAen_US
dc.contributor.authorChan, CWen_US
dc.contributor.authorIsseroff, RRen_US
dc.date.accessioned2012-06-26T05:57:19Z-
dc.date.available2012-06-26T05:57:19Z-
dc.date.issued2008en_US
dc.identifier.citationAmerican Journal Of Physiology - Renal Physiology, 2008, v. 294 n. 6, p. F1415-F1421en_US
dc.identifier.issn0363-6127en_US
dc.identifier.urihttp://hdl.handle.net/10722/149701-
dc.description.abstractAdult tissue stem cells replicate infrequently, retaining DNA nucleotide label (BrdU) for much longer periods than mature, dividing cells in which the label is diluted during a chase period. Those "label-retaining cells" (LRCs) have been identified as the tissue stem cells in skin, cornea, intestine, and prostate. However, in the urinary tract uroepithelial stem cells have not yet been identified. In this study, BrdU administration identified urothelial LRCs in the rat bladder with 9% of the epithelial basal cells retaining BrdU label 1 yr after its administration. Markers for stem cells in other tissues, Bcl, p63, cytokeratin 14, and β1 integrin, were immunolocalized in the basal bladder epithelium in or near urothelial LRCs, but not uniquely limited to these cells. Flow cytometry demonstrated that urothelial LRCs were small, had low granularity, and were uniquely β4 integrin bright. Urothelium from long-term labeled bladders was cultured and LRCs were found to be significantly more clonogenic and proliferative, characteristics of stem cells, than unlabeled urothelial cells. Thus, this work demonstrates that LRCs in the bladder localize to the basal layer, are small, low granularity, uniquely β4 integrin rich, slowly cycling and demonstrate superior clonogenic and proliferative ability compared with unlabeled epithelial cells. We propose that LRCs represent putative urothelial stem cells. Copyright © 2008 the American Physiological Society.en_US
dc.languageengen_US
dc.relation.ispartofAmerican Journal of Physiology - Renal Physiologyen_US
dc.subjectAdult Stem Cellsen_US
dc.subjectEpitheliumen_US
dc.subjectRatsen_US
dc.titleLabel-retaining cells of the bladder: Candidate urothelial stem cellsen_US
dc.typeArticleen_US
dc.identifier.emailChan, CW:camchan@hku.hken_US
dc.identifier.authorityChan, CW=rp01311en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1152/ajprenal.00533.2007en_US
dc.identifier.scopuseid_2-s2.0-48249148324en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-48249148324&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume294en_US
dc.identifier.issue6en_US
dc.identifier.spageF1415en_US
dc.identifier.epageF1421en_US
dc.identifier.isiWOS:000256469300020-
dc.identifier.f10001157601-
dc.identifier.scopusauthoridKurzrock, EA=7004184185en_US
dc.identifier.scopusauthoridLieu, DK=7003924538en_US
dc.identifier.scopusauthoridDeGraffenried, LA=6603605263en_US
dc.identifier.scopusauthoridChan, CW=12240386600en_US
dc.identifier.scopusauthoridIsseroff, RR=35589248200en_US

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