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Article: Proteomic comparison of nasopharyngeal cancer cell lines C666-1 and NP69 identifies down-regulation of annexin II and β 2-tubulin for nasopharyngeal carcinoma

TitleProteomic comparison of nasopharyngeal cancer cell lines C666-1 and NP69 identifies down-regulation of annexin II and β 2-tubulin for nasopharyngeal carcinoma
Authors
Issue Date2008
Citation
Archives Of Pathology And Laboratory Medicine, 2008, v. 132 n. 4, p. 675-683 How to Cite?
AbstractContext. - Nasopharyngeal carcinoma (NPC), common in southern China and North Africa, has a complex etiology involving interplay between viral, environmental, and hereditary factors and is almost constantly associated with the Epstein-Barr virus. Since the prognosis of locally advanced and metastatic diseases is poor, increased understanding of the pathogenesis of NPC would be important for discovering novel markers for patients' management. Objectives. - To compare the proteomic expression profile between an Epstein-Barr virus-associated NPC cell line (C666-1) and a normal NP cell line (NP69). The proteins with differential expression were analyzed in 40 undifferentiated NPC paraffin-embedded specimens. Design. - Differentially expressed proteins discovered between the two cell lines were identified by mass spectrometry. After confirmation by immunocytochemical staining, their expression in patient samples was measured using 40 pairs of undifferentiated NPCs together with their adjacent normal epithelia. Results. - Proteomic findings indicated that adenosine triphosphate synthase α chain was up-regulated, whereas annexin II, annexin V, β 2-tubulin, and profilin 1 were down-regulated. After confirming the results in agar-processed cell lines, annexin II and β 2-tubulin expression were found to be lower in tumor cells than in adjacent normal epithelial cells in 100% and 90% of the patients' specimens, respectively. Finally, annexin II down-regulation was positively associated with lymph node metastasis, suggesting that it may be a prognostic factor in NPC. Conclusions. - The results suggest that annexin II and β 2- tubulin down-regulation is important in NPC formation and may represent potential targets for further investigations.
Persistent Identifierhttp://hdl.handle.net/10722/149693
ISSN
2015 Impact Factor: 2.631
2015 SCImago Journal Rankings: 1.158
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, CMLen_US
dc.contributor.authorWong, SCCen_US
dc.contributor.authorLam, MYYen_US
dc.contributor.authorHui, EPen_US
dc.contributor.authorChan, JKCen_US
dc.contributor.authorLo, ESFen_US
dc.contributor.authorCheuk, Wen_US
dc.contributor.authorWong, MCKen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorChan, ATCen_US
dc.date.accessioned2012-06-26T05:57:10Z-
dc.date.available2012-06-26T05:57:10Z-
dc.date.issued2008en_US
dc.identifier.citationArchives Of Pathology And Laboratory Medicine, 2008, v. 132 n. 4, p. 675-683en_US
dc.identifier.issn0003-9985en_US
dc.identifier.urihttp://hdl.handle.net/10722/149693-
dc.description.abstractContext. - Nasopharyngeal carcinoma (NPC), common in southern China and North Africa, has a complex etiology involving interplay between viral, environmental, and hereditary factors and is almost constantly associated with the Epstein-Barr virus. Since the prognosis of locally advanced and metastatic diseases is poor, increased understanding of the pathogenesis of NPC would be important for discovering novel markers for patients' management. Objectives. - To compare the proteomic expression profile between an Epstein-Barr virus-associated NPC cell line (C666-1) and a normal NP cell line (NP69). The proteins with differential expression were analyzed in 40 undifferentiated NPC paraffin-embedded specimens. Design. - Differentially expressed proteins discovered between the two cell lines were identified by mass spectrometry. After confirmation by immunocytochemical staining, their expression in patient samples was measured using 40 pairs of undifferentiated NPCs together with their adjacent normal epithelia. Results. - Proteomic findings indicated that adenosine triphosphate synthase α chain was up-regulated, whereas annexin II, annexin V, β 2-tubulin, and profilin 1 were down-regulated. After confirming the results in agar-processed cell lines, annexin II and β 2-tubulin expression were found to be lower in tumor cells than in adjacent normal epithelial cells in 100% and 90% of the patients' specimens, respectively. Finally, annexin II down-regulation was positively associated with lymph node metastasis, suggesting that it may be a prognostic factor in NPC. Conclusions. - The results suggest that annexin II and β 2- tubulin down-regulation is important in NPC formation and may represent potential targets for further investigations.en_US
dc.languageengen_US
dc.relation.ispartofArchives of Pathology and Laboratory Medicineen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAnnexin A2 - Genetics - Metabolismen_US
dc.subject.meshAnnexin A5 - Genetics - Metabolismen_US
dc.subject.meshBiopsyen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshDown-Regulation - Geneticsen_US
dc.subject.meshEpithelial Cells - Metabolism - Pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshHerpesvirus 4, Human - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphatic Metastasisen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNasopharyngeal Neoplasms - Metabolism - Pathology - Virologyen_US
dc.subject.meshProfilins - Genetics - Metabolismen_US
dc.subject.meshProteomicsen_US
dc.subject.meshRna-Binding Proteins - Metabolismen_US
dc.subject.meshRibosomal Proteins - Metabolismen_US
dc.subject.meshTubulin - Genetics - Metabolismen_US
dc.subject.meshTumor Markers, Biological - Metabolismen_US
dc.titleProteomic comparison of nasopharyngeal cancer cell lines C666-1 and NP69 identifies down-regulation of annexin II and β 2-tubulin for nasopharyngeal carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid18384219-
dc.identifier.scopuseid_2-s2.0-41749085986en_US
dc.identifier.hkuros151296-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41749085986&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume132en_US
dc.identifier.issue4en_US
dc.identifier.spage675en_US
dc.identifier.epage683en_US
dc.identifier.isiWOS:000254729700016-
dc.identifier.scopusauthoridChan, CML=36096478600en_US
dc.identifier.scopusauthoridWong, SCC=26039647800en_US
dc.identifier.scopusauthoridLam, MYY=16836531700en_US
dc.identifier.scopusauthoridHui, EP=7005081895en_US
dc.identifier.scopusauthoridChan, JKC=7403287069en_US
dc.identifier.scopusauthoridLo, ESF=7101705996en_US
dc.identifier.scopusauthoridCheuk, W=7003958432en_US
dc.identifier.scopusauthoridWong, MCK=36476000300en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridChan, ATC=13404833700en_US

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