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Article: Adrenomedullin in the rat testis. I: Its production, actions on testosterone secretion, regulation by human chorionic gonadotropin, and its interaction with endothelin 1 in the Leydig cell

TitleAdrenomedullin in the rat testis. I: Its production, actions on testosterone secretion, regulation by human chorionic gonadotropin, and its interaction with endothelin 1 in the Leydig cell
Authors
KeywordsAdrenomedullin
Endothelin 1
Leydig cells
Testis
Issue Date2008
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2008, v. 78 n. 4, p. 773-779 How to Cite?
AbstractBased on the finding of gene expression of adrenomedullin (Adm) and its receptor components in the rat testis, a paracrine effect of ADM on testicular steroidogenesis has been suggested by our group. The present study demonstrates the gene expression of Adm and the effect of ADM on testosterone production in the Leydig cell. The regulation of ADM by hCG and its interaction with endothelin 1 (EDN1) in the rat Leydig cells are also observed. Primary culture of Leydig cells produced Adm mRNA and secreted 275 ± 19 pg immunoreactive ADM per 10 6 cells in 24 h. In addition, the Leydig cell was shown to coexpress mRNAs encoding for the calcitonin receptor-like receptor (CALCRL) and receptor activity-modifying protein (RAMP1, RAMP2, and RAMP3). These may account for the specific binding of ADM to the Leydig cells. Administration of ADM to Leydig cells resulted in an inhibition of hCG- and EDN1-stimulated testosterone production. Correlated with this, ADM reduced EDN1 production, whereas its production was increased by EDN1. Furthermore, the production of ADM and the mRNA levels of Calcrl and Ramp2 were suppressed by hCG. Our results suggest that ADM has an autocrine effect on Leydig cell steroidogenesis, possibly by interacting with EDN1 and under the control of gonadotropin. We propose that there is an ADM/EDN1 local regulatory mechanism that may be important in modulating the control of testicular functions by gonadotropins. © 2008 by the Society for the Study of Reproduction, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/149691
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, YFen_US
dc.contributor.authorO, WSen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2012-06-26T05:57:09Z-
dc.date.available2012-06-26T05:57:09Z-
dc.date.issued2008en_US
dc.identifier.citationBiology Of Reproduction, 2008, v. 78 n. 4, p. 773-779en_US
dc.identifier.issn0006-3363en_US
dc.identifier.urihttp://hdl.handle.net/10722/149691-
dc.description.abstractBased on the finding of gene expression of adrenomedullin (Adm) and its receptor components in the rat testis, a paracrine effect of ADM on testicular steroidogenesis has been suggested by our group. The present study demonstrates the gene expression of Adm and the effect of ADM on testosterone production in the Leydig cell. The regulation of ADM by hCG and its interaction with endothelin 1 (EDN1) in the rat Leydig cells are also observed. Primary culture of Leydig cells produced Adm mRNA and secreted 275 ± 19 pg immunoreactive ADM per 10 6 cells in 24 h. In addition, the Leydig cell was shown to coexpress mRNAs encoding for the calcitonin receptor-like receptor (CALCRL) and receptor activity-modifying protein (RAMP1, RAMP2, and RAMP3). These may account for the specific binding of ADM to the Leydig cells. Administration of ADM to Leydig cells resulted in an inhibition of hCG- and EDN1-stimulated testosterone production. Correlated with this, ADM reduced EDN1 production, whereas its production was increased by EDN1. Furthermore, the production of ADM and the mRNA levels of Calcrl and Ramp2 were suppressed by hCG. Our results suggest that ADM has an autocrine effect on Leydig cell steroidogenesis, possibly by interacting with EDN1 and under the control of gonadotropin. We propose that there is an ADM/EDN1 local regulatory mechanism that may be important in modulating the control of testicular functions by gonadotropins. © 2008 by the Society for the Study of Reproduction, Inc.en_US
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_US
dc.relation.ispartofBiology of Reproductionen_US
dc.subjectAdrenomedullin-
dc.subjectEndothelin 1-
dc.subjectLeydig cells-
dc.subjectTestis-
dc.subject.meshAdrenomedullin - Biosynthesis - Genetics - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCalcitonin Receptor-Like Proteinen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChorionic Gonadotropin - Pharmacologyen_US
dc.subject.meshDrug Interactionsen_US
dc.subject.meshEndothelin-1 - Pharmacologyen_US
dc.subject.meshGene Expression - Drug Effectsen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Geneticsen_US
dc.subject.meshLeydig Cells - Chemistry - Drug Effects - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Geneticsen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptor Activity-Modifying Protein 1en_US
dc.subject.meshReceptor Activity-Modifying Protein 2en_US
dc.subject.meshReceptor Activity-Modifying Protein 3en_US
dc.subject.meshReceptor Activity-Modifying Proteinsen_US
dc.subject.meshReceptors, Adrenomedullinen_US
dc.subject.meshReceptors, Calcitonin - Geneticsen_US
dc.subject.meshReceptors, G-Protein-Coupled - Geneticsen_US
dc.subject.meshTestis - Drug Effects - Metabolismen_US
dc.subject.meshTestosterone - Secretionen_US
dc.titleAdrenomedullin in the rat testis. I: Its production, actions on testosterone secretion, regulation by human chorionic gonadotropin, and its interaction with endothelin 1 in the Leydig cellen_US
dc.typeArticleen_US
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_US
dc.identifier.emailTang, F:ftang@hkucc.hku.hken_US
dc.identifier.authorityO, WS=rp00315en_US
dc.identifier.authorityTang, F=rp00327en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1095/biolreprod.107.060871en_US
dc.identifier.pmid18094363-
dc.identifier.scopuseid_2-s2.0-41549127120en_US
dc.identifier.hkuros151740-
dc.identifier.hkuros141397-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41549127120&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume78en_US
dc.identifier.issue4en_US
dc.identifier.spage773en_US
dc.identifier.epage779en_US
dc.identifier.eissn1529-7268-
dc.identifier.isiWOS:000254217500023-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, YF=7403676061en_US
dc.identifier.scopusauthoridO, WS=6701729369en_US
dc.identifier.scopusauthoridTang, F=7201979770en_US

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