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Article: Hormonal regulation of Gα i2 and mPRα in immortalized human oviductal cell line OE-E6/E7

TitleHormonal regulation of Gα i2 and mPRα in immortalized human oviductal cell line OE-E6/E7
Authors
KeywordsEstradiol
Fallopian tube
G protein
Progesterone
Regulation
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2007, v. 13 n. 12, p. 845-851 How to Cite?
AbstractHeterotrimeric G proteins play a key role in membrane-mediated cell-signalling and hormonal regulation. Our earlier studies gave evidence of G protein subunit Gα i2 being under hormonal regulation in human in vivo. In this study, we used immortalized human oviduct epithelial cell line OE-E6/E7 as a model to study the hormonal regulation of Gα i2. We aimed at clarifying whether estradiol or progesterone could individually regulate the expression of Gα i2 and its potential signalling partners. Furthermore, we aimed to investigate which sex hormone receptors could potentially mediate the gene regulation in OE-E6/E7 cell line. OE-E6/E7 cells were cultured for 5 days with different concentrations of estradiol or progesterone. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using cDNA of the hormone-treated cells to reveal any changes in gene expression. The presence of potential receptor targets in these cells was studied using PCR. Our data clearly showed that low concentrations of estradiol up-regulated the expression of Gα i2 gene and down-regulated the expression of membrane progesterone receptor mPRα gene in OE-E6/E7 cell line. Progesterone had no significant effect on Gα i2 gene expression, but it caused up-regulation of mPRα gene expression. In conclusion, it appears that sex hormones regulate the expression of Gα i2 and mPRα genes in a reverse manner in OE-E6/E7 cells. Our results suggest that estrogen receptor ERβ mediates the regulatory effects of estradiol in these cells. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/149683
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMönkkönen, KSen_HK
dc.contributor.authorAflatoonian, Ren_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLaitinen, JTen_HK
dc.contributor.authorFazeli, Aen_HK
dc.date.accessioned2012-06-26T05:57:04Z-
dc.date.available2012-06-26T05:57:04Z-
dc.date.issued2007en_HK
dc.identifier.citationMolecular Human Reproduction, 2007, v. 13 n. 12, p. 845-851en_HK
dc.identifier.issn1360-9947en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149683-
dc.description.abstractHeterotrimeric G proteins play a key role in membrane-mediated cell-signalling and hormonal regulation. Our earlier studies gave evidence of G protein subunit Gα i2 being under hormonal regulation in human in vivo. In this study, we used immortalized human oviduct epithelial cell line OE-E6/E7 as a model to study the hormonal regulation of Gα i2. We aimed at clarifying whether estradiol or progesterone could individually regulate the expression of Gα i2 and its potential signalling partners. Furthermore, we aimed to investigate which sex hormone receptors could potentially mediate the gene regulation in OE-E6/E7 cell line. OE-E6/E7 cells were cultured for 5 days with different concentrations of estradiol or progesterone. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using cDNA of the hormone-treated cells to reveal any changes in gene expression. The presence of potential receptor targets in these cells was studied using PCR. Our data clearly showed that low concentrations of estradiol up-regulated the expression of Gα i2 gene and down-regulated the expression of membrane progesterone receptor mPRα gene in OE-E6/E7 cell line. Progesterone had no significant effect on Gα i2 gene expression, but it caused up-regulation of mPRα gene expression. In conclusion, it appears that sex hormones regulate the expression of Gα i2 and mPRα genes in a reverse manner in OE-E6/E7 cells. Our results suggest that estrogen receptor ERβ mediates the regulatory effects of estradiol in these cells. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_HK
dc.relation.ispartofMolecular Human Reproductionen_HK
dc.rightsMolecular Human Reproduction. Copyright © Oxford University Press-
dc.subjectEstradiolen_HK
dc.subjectFallopian tubeen_HK
dc.subjectG proteinen_HK
dc.subjectProgesteroneen_HK
dc.subjectRegulationen_HK
dc.titleHormonal regulation of Gα i2 and mPRα in immortalized human oviductal cell line OE-E6/E7en_HK
dc.typeArticleen_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/molehr/gam075en_HK
dc.identifier.pmid17977902-
dc.identifier.scopuseid_2-s2.0-37549056555en_HK
dc.identifier.hkuros139646-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-37549056555&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue12en_HK
dc.identifier.spage845en_HK
dc.identifier.epage851en_HK
dc.identifier.isiWOS:000251907500002-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMönkkönen, KS=8901685600en_HK
dc.identifier.scopusauthoridAflatoonian, R=16744883000en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLaitinen, JT=7103329968en_HK
dc.identifier.scopusauthoridFazeli, A=7003548599en_HK

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