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Article: Characterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicity

TitleCharacterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicity
Authors
KeywordsΒ-Amyloid Peptide
Arabinogalactan-Protein
Fructus Lycii
Neuronal Apoptosis
Neurotoxicity
Issue Date2007
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2007, v. 20 n. 2, p. 261-268 How to Cite?
AbstractAlzheimer's disease (AD) is an age-related neurodegenerative disease. There are increasing lines of evidence showing that the molecular signaling pathways in aged cells are altered so that cells are susceptible to injury. We and other laboratories have demonstrated the significant involvement of double-stranded RNA-dependent protein kinase (PKR) in β-amyloid (Aβ) peptide neurotoxicity and in AD. Fructus lycii (the fruit of Lycium barbarum) has long been used in oriental medicine as an anti-aging agent. Our previous studies demonstrated that the aqueous extract isolated from L. barbarum exhibited significant protection on cultured neurons against harmful chemical toxins such as Aβ and dithiothreitol. We also showed that the polysaccharide-containing extract (LBP) from L. barbarum exhibited neuroprotective effects in the retina against ocular hypertension in a laser-induced glaucoma animal model. In this study, we aimed to investigate whether LBP can elicit neuroprotection to neurons stressed by Aβ peptides. Furthermore, we planned to isolate and identify the neuroprotective agent from LBP using chromatographic methods. Our results showed that pretreatment of LBP effectively protected neurons against Aβ-induced apoptosis by reducing the activity of both caspase-3 and -2, but not caspase-8 and -9. A new arabino-galactan-protein (LBP-III) was isolated from LBP and attenuated Aβ peptide-activated caspase-3-like activity. LBP-III markedly reduced the phosphorylation of PKR triggered by Aβ peptide. Since the phosphorylation state of PKR increased with age, reduction of its phosphorylation triggered by Aβ peptide may implicate that LBP-III from Fructus lycii is a potential neuroprotective agent in AD. As herbal medicine has received increasing attention for the treatment of AD, our study will open a window for the development of a neuroprotective agent for anti-aging from Chinese medicine.
Persistent Identifierhttp://hdl.handle.net/10722/149677
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, MSen_US
dc.contributor.authorLai, CSWen_US
dc.contributor.authorHo, YSen_US
dc.contributor.authorZee, SYen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorYuen, WHen_US
dc.contributor.authorChang, RCCen_US
dc.date.accessioned2012-06-26T05:56:58Z-
dc.date.available2012-06-26T05:56:58Z-
dc.date.issued2007en_US
dc.identifier.citationInternational Journal Of Molecular Medicine, 2007, v. 20 n. 2, p. 261-268en_US
dc.identifier.issn1107-3756en_US
dc.identifier.urihttp://hdl.handle.net/10722/149677-
dc.description.abstractAlzheimer's disease (AD) is an age-related neurodegenerative disease. There are increasing lines of evidence showing that the molecular signaling pathways in aged cells are altered so that cells are susceptible to injury. We and other laboratories have demonstrated the significant involvement of double-stranded RNA-dependent protein kinase (PKR) in β-amyloid (Aβ) peptide neurotoxicity and in AD. Fructus lycii (the fruit of Lycium barbarum) has long been used in oriental medicine as an anti-aging agent. Our previous studies demonstrated that the aqueous extract isolated from L. barbarum exhibited significant protection on cultured neurons against harmful chemical toxins such as Aβ and dithiothreitol. We also showed that the polysaccharide-containing extract (LBP) from L. barbarum exhibited neuroprotective effects in the retina against ocular hypertension in a laser-induced glaucoma animal model. In this study, we aimed to investigate whether LBP can elicit neuroprotection to neurons stressed by Aβ peptides. Furthermore, we planned to isolate and identify the neuroprotective agent from LBP using chromatographic methods. Our results showed that pretreatment of LBP effectively protected neurons against Aβ-induced apoptosis by reducing the activity of both caspase-3 and -2, but not caspase-8 and -9. A new arabino-galactan-protein (LBP-III) was isolated from LBP and attenuated Aβ peptide-activated caspase-3-like activity. LBP-III markedly reduced the phosphorylation of PKR triggered by Aβ peptide. Since the phosphorylation state of PKR increased with age, reduction of its phosphorylation triggered by Aβ peptide may implicate that LBP-III from Fructus lycii is a potential neuroprotective agent in AD. As herbal medicine has received increasing attention for the treatment of AD, our study will open a window for the development of a neuroprotective agent for anti-aging from Chinese medicine.en_US
dc.languageengen_US
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_US
dc.relation.ispartofInternational Journal of Molecular Medicineen_US
dc.subjectΒ-Amyloid Peptideen_US
dc.subjectArabinogalactan-Proteinen_US
dc.subjectFructus Lyciien_US
dc.subjectNeuronal Apoptosisen_US
dc.subjectNeurotoxicityen_US
dc.titleCharacterization of the effects of anti-aging medicine Fructus lycii on β-amyloid peptide neurotoxicityen_US
dc.typeArticleen_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid17611646-
dc.identifier.scopuseid_2-s2.0-35448999375en_US
dc.identifier.hkuros133028-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-35448999375&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume20en_US
dc.identifier.issue2en_US
dc.identifier.spage261en_US
dc.identifier.epage268en_US
dc.identifier.isiWOS:000248282800017-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridYu, MS=35346047600en_US
dc.identifier.scopusauthoridLai, CSW=26022547000en_US
dc.identifier.scopusauthoridHo, YS=14031513600en_US
dc.identifier.scopusauthoridZee, SY=7004001733en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridYuen, WH=7102761282en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US

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