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Article: Chemotactic effect of ciliary neurotrophic factor on macrophages in retinal ganglion cell survival and axonal regeneration

TitleChemotactic effect of ciliary neurotrophic factor on macrophages in retinal ganglion cell survival and axonal regeneration
Authors
Issue Date2007
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2007, v. 48 n. 9, p. 4257-4266 How to Cite?
AbstractPURPOSE. To examine whether ciliary neurotrophic factor (CNTF) has a chemotactic effect on macrophages and whether macrophages are involved in CNTF-induced retinal ganglion cell (RGC) survival and axonal regeneration after optic nerve (ON) injury. METHODS. Adult Fischer 344 rats received an autologous peripheral nerve graft onto transected ON for injured axons to grow. CNTF was applied intravitreally. When needed, clodronate liposomes were applied intravitreally or intravenously to deplete macrophages in the eye. A chemotaxis microchamber system was used to examine whether CNTF has a chemotactic effect on macrophages in vitro, whereas immunohistochemistry was used to identify the location of macrophages/microglia in the retina. The effects of CNTF on RGC neurite outgrowth and macrophage/microglia proliferation were tested in retinal explants. RESULTS. Intravitreal CNTF significantly enhanced RGC survival and axonal regeneration as well as the number of macrophages in the eye. Removal of macrophages significantly reduced CNTF-induced RGC survival and axon regeneration. A chemotaxis assay showed a clear chemotactic effect of CNTF on blood-derived but not peritoneal macrophages. Immunohistochemistry revealed that local microglia was located in a region from the nerve fiber layer (NFL) to the inner nuclear layer, whereas blood-derived macrophages were in the NFL. In vitro experiments revealed that CNTF did not enhance neurite outgrowth or macrophage/microglia proliferation in retinal explants. CONCLUSIONS. CNTF is a chemoattractant but not a proliferation enhancer for blood-derived macrophages, and blood-borne macrophages recruited into the eye by CNTF participate in RGC protection. This finding thus adds an important category to the existing understanding of the biological actions of CNTF. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/149675
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCen, LPen_US
dc.contributor.authorLuo, JMen_US
dc.contributor.authorZhang, CWen_US
dc.contributor.authorFan, YMen_US
dc.contributor.authorSong, Yen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorVan Rooijen, Nen_US
dc.contributor.authorPang, CPen_US
dc.contributor.authorLam, DSCen_US
dc.contributor.authorCui, Qen_US
dc.date.accessioned2012-06-26T05:56:56Z-
dc.date.available2012-06-26T05:56:56Z-
dc.date.issued2007en_US
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2007, v. 48 n. 9, p. 4257-4266en_US
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://hdl.handle.net/10722/149675-
dc.description.abstractPURPOSE. To examine whether ciliary neurotrophic factor (CNTF) has a chemotactic effect on macrophages and whether macrophages are involved in CNTF-induced retinal ganglion cell (RGC) survival and axonal regeneration after optic nerve (ON) injury. METHODS. Adult Fischer 344 rats received an autologous peripheral nerve graft onto transected ON for injured axons to grow. CNTF was applied intravitreally. When needed, clodronate liposomes were applied intravitreally or intravenously to deplete macrophages in the eye. A chemotaxis microchamber system was used to examine whether CNTF has a chemotactic effect on macrophages in vitro, whereas immunohistochemistry was used to identify the location of macrophages/microglia in the retina. The effects of CNTF on RGC neurite outgrowth and macrophage/microglia proliferation were tested in retinal explants. RESULTS. Intravitreal CNTF significantly enhanced RGC survival and axonal regeneration as well as the number of macrophages in the eye. Removal of macrophages significantly reduced CNTF-induced RGC survival and axon regeneration. A chemotaxis assay showed a clear chemotactic effect of CNTF on blood-derived but not peritoneal macrophages. Immunohistochemistry revealed that local microglia was located in a region from the nerve fiber layer (NFL) to the inner nuclear layer, whereas blood-derived macrophages were in the NFL. In vitro experiments revealed that CNTF did not enhance neurite outgrowth or macrophage/microglia proliferation in retinal explants. CONCLUSIONS. CNTF is a chemoattractant but not a proliferation enhancer for blood-derived macrophages, and blood-borne macrophages recruited into the eye by CNTF participate in RGC protection. This finding thus adds an important category to the existing understanding of the biological actions of CNTF. Copyright © Association for Research in Vision and Ophthalmology.en_US
dc.languageengen_US
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_US
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_US
dc.titleChemotactic effect of ciliary neurotrophic factor on macrophages in retinal ganglion cell survival and axonal regenerationen_US
dc.typeArticleen_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1167/iovs.06-0791en_US
dc.identifier.pmid17724215-
dc.identifier.scopuseid_2-s2.0-34548016256en_US
dc.identifier.hkuros150998-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548016256&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume48en_US
dc.identifier.issue9en_US
dc.identifier.spage4257en_US
dc.identifier.epage4266en_US
dc.identifier.isiWOS:000249061900050-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCen, LP=36856212100en_US
dc.identifier.scopusauthoridLuo, JM=51562038700en_US
dc.identifier.scopusauthoridZhang, CW=51562967800en_US
dc.identifier.scopusauthoridFan, YM=19337163200en_US
dc.identifier.scopusauthoridSong, Y=51562462900en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridVan Rooijen, N=35428581800en_US
dc.identifier.scopusauthoridPang, CP=7201425127en_US
dc.identifier.scopusauthoridLam, DSC=35500200200en_US
dc.identifier.scopusauthoridCui, Q=7103080164en_US

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