File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterizing the neuroprotective effects of alkaline extract of Lycium barbarum on β-amyloid peptide neurotoxicity

TitleCharacterizing the neuroprotective effects of alkaline extract of Lycium barbarum on β-amyloid peptide neurotoxicity
Authors
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2007, v. 1158 n. 1, p. 123-134 How to Cite?
AbstractLycium barbarum is an oriental medicinal herb that has long been used for its anti-aging and cell-protective properties. Previous studies have shown that aqueous extracts from L. barbarum exhibit neuroprotection via inhibiting pro-apoptotic signaling pathways. Other active components can also be accomplished by novel alkaline extraction method, which may give different profiles of water-soluble components. We hypothesize that another active component obtained by alkaline extraction method exerts different biological mechanisms to protect neurons. In this study, we aim to examine the neuroprotective effects from the alkaline extract of L. barbarum, namely LBB, to attenuate β-amyloid (Aβ) peptide neurotoxicity. Primary cortical neurons were exposed to Aβ-peptides inducing apoptosis and neuronal cell death. Pretreatment of LBB significantly reduced the level of lactate dehydrogenase (LDH) release and the activity of caspase-3 triggered by Aβ. "Wash-out" procedures did not reduce its neuroprotective effects, suggesting that LBB may not bind directly to Aβ. We have further isolated three subfractions from LBB, namely LBB-0, LBB-I and LBB-II. LBB-I and LBB-II showed differential neuroprotective effects. Western blot analysis demonstrated that LBB-I and LBB-II markedly enhanced the phosphorylation of Akt. Taken together, our results suggested that the glycoconjugate isolated from novel alkaline extraction method can open up a new avenue for drug discovery in neurodegenerative diseases. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/149671
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, YSen_US
dc.contributor.authorYu, MSen_US
dc.contributor.authorLai, CSWen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorYuen, WHen_US
dc.contributor.authorChang, RCCen_US
dc.date.accessioned2012-06-26T05:56:50Z-
dc.date.available2012-06-26T05:56:50Z-
dc.date.issued2007en_US
dc.identifier.citationBrain Research, 2007, v. 1158 n. 1, p. 123-134en_US
dc.identifier.issn0006-8993en_US
dc.identifier.urihttp://hdl.handle.net/10722/149671-
dc.description.abstractLycium barbarum is an oriental medicinal herb that has long been used for its anti-aging and cell-protective properties. Previous studies have shown that aqueous extracts from L. barbarum exhibit neuroprotection via inhibiting pro-apoptotic signaling pathways. Other active components can also be accomplished by novel alkaline extraction method, which may give different profiles of water-soluble components. We hypothesize that another active component obtained by alkaline extraction method exerts different biological mechanisms to protect neurons. In this study, we aim to examine the neuroprotective effects from the alkaline extract of L. barbarum, namely LBB, to attenuate β-amyloid (Aβ) peptide neurotoxicity. Primary cortical neurons were exposed to Aβ-peptides inducing apoptosis and neuronal cell death. Pretreatment of LBB significantly reduced the level of lactate dehydrogenase (LDH) release and the activity of caspase-3 triggered by Aβ. "Wash-out" procedures did not reduce its neuroprotective effects, suggesting that LBB may not bind directly to Aβ. We have further isolated three subfractions from LBB, namely LBB-0, LBB-I and LBB-II. LBB-I and LBB-II showed differential neuroprotective effects. Western blot analysis demonstrated that LBB-I and LBB-II markedly enhanced the phosphorylation of Akt. Taken together, our results suggested that the glycoconjugate isolated from novel alkaline extraction method can open up a new avenue for drug discovery in neurodegenerative diseases. © 2007 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_US
dc.relation.ispartofBrain Researchen_US
dc.subject.meshAmyloid Beta-Peptides - Toxicityen_US
dc.subject.meshAnalysis Of Varianceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCaspase 3 - Metabolismen_US
dc.subject.meshCell Counten_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCerebral Cortex - Cytologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshDrug Interactionsen_US
dc.subject.meshDrugs, Chinese Herbal - Pharmacologyen_US
dc.subject.meshEmbryo, Mammalianen_US
dc.subject.meshIndoles - Diagnostic Useen_US
dc.subject.meshL-Lactate Dehydrogenase - Metabolismen_US
dc.subject.meshLycium - Chemistryen_US
dc.subject.meshNeurons - Drug Effectsen_US
dc.subject.meshNeuroprotective Agents - Pharmacologyen_US
dc.subject.meshPeptide Fragments - Toxicityen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSignal Transduction - Drug Effectsen_US
dc.titleCharacterizing the neuroprotective effects of alkaline extract of Lycium barbarum on β-amyloid peptide neurotoxicityen_US
dc.typeArticleen_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.brainres.2007.04.075en_US
dc.identifier.pmid17568570-
dc.identifier.scopuseid_2-s2.0-34447542840en_US
dc.identifier.hkuros133026-
dc.identifier.hkuros148365-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447542840&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1158en_US
dc.identifier.issue1en_US
dc.identifier.spage123en_US
dc.identifier.epage134en_US
dc.identifier.isiWOS:000248710400013-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridHo, YS=14031513600en_US
dc.identifier.scopusauthoridYu, MS=35346047600en_US
dc.identifier.scopusauthoridLai, CSW=26022547000en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridYuen, WH=7102761282en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats