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Article: Identification of MELla melatonin receptors using a peptide specific polyclonal antibody

TitleIdentification of MELla melatonin receptors using a peptide specific polyclonal antibody
Authors
Issue Date1996
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1996, v. 10 n. 3, p. A413 How to Cite?
AbstractMelatonin plays an important role in regulating arcadian rhythms in various species including man. Its biologic action is mediated through specific receptors (MELR) which have been found in the CNS as well as in other tissues, such as kidney. To gain insight into how melatonin regulates kidney function we sought to identify its renal subcellular localization. Accordingly we prepared a polyclonal antibody to a highly conserved peptide derived from the predicted intracellular loop of the human brain MELla receptor (Neuron 13, 1995). Immunoblotting studies were conducted on brush border (BBM) and basolateral membranes (BLM) from guinea pig kidney as well as human embryonic (HEK) and opossum (OK) kidney cells, and compared with preimmune serum. A specific peptide blockable band of MW 37-38 kDa was detected in BBM, BLM and HEK cells, but not in OK cells. No band was observed with preimmune serum. The presence of this 37-38 kDa band correlated with the presence of high affinity MELR binding sites determined by radioligand binding using iodinated melatonin. These data demonstrate the specificity of the anti MELR antibody. The renal localization of MELR suggests that melatonin may have an affect on cortical tubular function. Further studies are planned to map MELR in other regions of the kidney as well as other epithelia.
Persistent Identifierhttp://hdl.handle.net/10722/149663
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorSong, Yen_US
dc.contributor.authorChan, CWYen_US
dc.contributor.authorPang, SFen_US
dc.contributor.authorBrown, GMen_US
dc.contributor.authorSilverman, Men_US
dc.date.accessioned2012-06-26T05:56:44Z-
dc.date.available2012-06-26T05:56:44Z-
dc.date.issued1996en_US
dc.identifier.citationFaseb Journal, 1996, v. 10 n. 3, p. A413en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/149663-
dc.description.abstractMelatonin plays an important role in regulating arcadian rhythms in various species including man. Its biologic action is mediated through specific receptors (MELR) which have been found in the CNS as well as in other tissues, such as kidney. To gain insight into how melatonin regulates kidney function we sought to identify its renal subcellular localization. Accordingly we prepared a polyclonal antibody to a highly conserved peptide derived from the predicted intracellular loop of the human brain MELla receptor (Neuron 13, 1995). Immunoblotting studies were conducted on brush border (BBM) and basolateral membranes (BLM) from guinea pig kidney as well as human embryonic (HEK) and opossum (OK) kidney cells, and compared with preimmune serum. A specific peptide blockable band of MW 37-38 kDa was detected in BBM, BLM and HEK cells, but not in OK cells. No band was observed with preimmune serum. The presence of this 37-38 kDa band correlated with the presence of high affinity MELR binding sites determined by radioligand binding using iodinated melatonin. These data demonstrate the specificity of the anti MELR antibody. The renal localization of MELR suggests that melatonin may have an affect on cortical tubular function. Further studies are planned to map MELR in other regions of the kidney as well as other epithelia.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleIdentification of MELla melatonin receptors using a peptide specific polyclonal antibodyen_US
dc.typeArticleen_US
dc.identifier.emailChan, CWY:camchan@hku.hken_US
dc.identifier.authorityChan, CWY=rp01311en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33749139304en_US
dc.identifier.volume10en_US
dc.identifier.issue3en_US
dc.identifier.spageA413en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSong, Y=7404920869en_US
dc.identifier.scopusauthoridChan, CWY=12240386600en_US
dc.identifier.scopusauthoridPang, SF=7402528719en_US
dc.identifier.scopusauthoridBrown, GM=35493704500en_US
dc.identifier.scopusauthoridSilverman, M=7403299191en_US

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