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Article: The survival differentiation and migration of skin-derived neural stem cells in injured spinal cord of rat

TitleThe survival differentiation and migration of skin-derived neural stem cells in injured spinal cord of rat
Authors
KeywordsDifferentiation
Immunocytochemistry
Neural Stem Cell
Rat
Skin Stem Cell
Spinal Cord Injury
Survival
Issue Date2006
Citation
Acta Anatomica Sinica, 2006, v. 37 n. 3, p. 281-284 How to Cite?
AbstractObjective: To investigate whether the skin-derived neural stem cells inducted by the culture could survive, differentiate and migrate in the lesioned site of rat spinal cord. Methods: The skin of new born rat trans-gene of green fluorescence protein(GFP) was applied to be dissociated into cells, cultured and inducted to proliferate in vitro. Skin-derived neural stem cells were identified by immunocytochemistry staining. Then, skin-derived neural stem cells inducted by the culture were transplanted into the lesioned site of rat spinal cord hemisection. Thirty days and sixty days after the operation, the survival, migration and differentiation of transplanted cells were observed by immunocytochemistry staining. Results: Ten days at cultured skin-dissociated cells, many cell-spheres had been formed by the proliferation of suspensive growing cells. These cell-spheres showed nestin positive staining of immunocytochemistry. It suggestes that the cell-spheres are neurospheres. In vivo, many transplanted skin-derived neural stem cells with GFP were observed in the lesioned area of spinal cord. Some transplanted cells migrated into host spinal cord tissue far away from the lesioned area. Some transplanted surviving cells showed nestin, MAP2 and GFAP positive staining of immunocytochemistry separately. Conclusion: Skin-derived ne ural stem cells inducted by the culture may survive, migrate and differentiate into neuron-like cells and astrocyte-like cells in injured spinal cord of rat.
Persistent Identifierhttp://hdl.handle.net/10722/149658
ISSN
2015 SCImago Journal Rankings: 0.121
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Wen_US
dc.contributor.authorZeng, YSen_US
dc.contributor.authorWu, WTen_US
dc.contributor.authorChen, YYen_US
dc.contributor.authorChen, SJen_US
dc.date.accessioned2012-06-26T05:56:40Z-
dc.date.available2012-06-26T05:56:40Z-
dc.date.issued2006en_US
dc.identifier.citationActa Anatomica Sinica, 2006, v. 37 n. 3, p. 281-284en_US
dc.identifier.issn0529-1356en_US
dc.identifier.urihttp://hdl.handle.net/10722/149658-
dc.description.abstractObjective: To investigate whether the skin-derived neural stem cells inducted by the culture could survive, differentiate and migrate in the lesioned site of rat spinal cord. Methods: The skin of new born rat trans-gene of green fluorescence protein(GFP) was applied to be dissociated into cells, cultured and inducted to proliferate in vitro. Skin-derived neural stem cells were identified by immunocytochemistry staining. Then, skin-derived neural stem cells inducted by the culture were transplanted into the lesioned site of rat spinal cord hemisection. Thirty days and sixty days after the operation, the survival, migration and differentiation of transplanted cells were observed by immunocytochemistry staining. Results: Ten days at cultured skin-dissociated cells, many cell-spheres had been formed by the proliferation of suspensive growing cells. These cell-spheres showed nestin positive staining of immunocytochemistry. It suggestes that the cell-spheres are neurospheres. In vivo, many transplanted skin-derived neural stem cells with GFP were observed in the lesioned area of spinal cord. Some transplanted cells migrated into host spinal cord tissue far away from the lesioned area. Some transplanted surviving cells showed nestin, MAP2 and GFAP positive staining of immunocytochemistry separately. Conclusion: Skin-derived ne ural stem cells inducted by the culture may survive, migrate and differentiate into neuron-like cells and astrocyte-like cells in injured spinal cord of rat.en_US
dc.languageengen_US
dc.relation.ispartofActa Anatomica Sinicaen_US
dc.subjectDifferentiationen_US
dc.subjectImmunocytochemistryen_US
dc.subjectNeural Stem Cellen_US
dc.subjectRaten_US
dc.subjectSkin Stem Cellen_US
dc.subjectSpinal Cord Injuryen_US
dc.subjectSurvivalen_US
dc.titleThe survival differentiation and migration of skin-derived neural stem cells in injured spinal cord of raten_US
dc.typeArticleen_US
dc.identifier.emailWu, WT:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, WT=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33746389203en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746389203&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume37en_US
dc.identifier.issue3en_US
dc.identifier.spage281en_US
dc.identifier.epage284en_US
dc.identifier.scopusauthoridZhang, W=36076785500en_US
dc.identifier.scopusauthoridZeng, YS=8236229300en_US
dc.identifier.scopusauthoridWu, WT=7407081122en_US
dc.identifier.scopusauthoridChen, YY=14049679100en_US
dc.identifier.scopusauthoridChen, SJ=12809083300en_US

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