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Article: Adrenomedullin peptide: Gene expression of adrenomedullin, its receptors and receptor activity modifying proteins, and receptor binding in rat testis - Actions on testosterone secretion

TitleAdrenomedullin peptide: Gene expression of adrenomedullin, its receptors and receptor activity modifying proteins, and receptor binding in rat testis - Actions on testosterone secretion
Authors
KeywordsAging
Human chorionic gonadotropin
Testis
Testosterone
Issue Date2006
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2006, v. 75 n. 2, p. 183-188 How to Cite?
AbstractAdrenomedullin (ADM) has been shown to be present in the human and rat male reproductive systems. This study demonstrates the expression of ADM in the rat testis and its effect on the secretion of testosterone. Whole testicular extracts had 5.43 ± 0.42 fmol of immunoreactive ADM per milligram of protein and 84 ± 8 fg of ADM mRNA per picogram of Actb (β-actin) mRNA. Immunocytochemical studies showed positive ADM immunostaining in the Leydig cells and in the Sertoli cells. Gel filtration chromatography of testicular extracts showed two peaks, with the predominant one eluting at the position of the ADM precursor. Furthermore, the testis was shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity modifying protein 1 (Ramp1), Ramp2, and Ramp3. These account for the specific binding of ADM to the testis, which was partially inhibited by human ADM (22-52) and by human calcitonin gene-related peptide (8-37), the ADM and calcitonin gene-related peptide receptor antagonists, respectively. Administration of ADM to testicular blocks in vitro resulted in a dose-dependent inhibition of hCG-stimulated release of testosterone, which was abolished by the administration of ADM (22-52). Our results suggest a paracrine effect of ADM on testicular steroidogenesis. © 2006 by the Society for the Study of Reproduction, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/149657
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, YYen_HK
dc.contributor.authorHwang, ISSen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorTang, Fen_HK
dc.date.accessioned2012-06-26T05:56:39Z-
dc.date.available2012-06-26T05:56:39Z-
dc.date.issued2006en_HK
dc.identifier.citationBiology Of Reproduction, 2006, v. 75 n. 2, p. 183-188en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149657-
dc.description.abstractAdrenomedullin (ADM) has been shown to be present in the human and rat male reproductive systems. This study demonstrates the expression of ADM in the rat testis and its effect on the secretion of testosterone. Whole testicular extracts had 5.43 ± 0.42 fmol of immunoreactive ADM per milligram of protein and 84 ± 8 fg of ADM mRNA per picogram of Actb (β-actin) mRNA. Immunocytochemical studies showed positive ADM immunostaining in the Leydig cells and in the Sertoli cells. Gel filtration chromatography of testicular extracts showed two peaks, with the predominant one eluting at the position of the ADM precursor. Furthermore, the testis was shown to coexpress mRNAs encoding the calcitonin receptor-like receptor and receptor activity modifying protein 1 (Ramp1), Ramp2, and Ramp3. These account for the specific binding of ADM to the testis, which was partially inhibited by human ADM (22-52) and by human calcitonin gene-related peptide (8-37), the ADM and calcitonin gene-related peptide receptor antagonists, respectively. Administration of ADM to testicular blocks in vitro resulted in a dose-dependent inhibition of hCG-stimulated release of testosterone, which was abolished by the administration of ADM (22-52). Our results suggest a paracrine effect of ADM on testicular steroidogenesis. © 2006 by the Society for the Study of Reproduction, Inc.en_HK
dc.languageengen_US
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.subjectAgingen_HK
dc.subjectHuman chorionic gonadotropinen_HK
dc.subjectTestisen_HK
dc.subjectTestosteroneen_HK
dc.subject.meshAdrenomedullin - Genetics - Metabolism - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCalcitonin Receptor-Like Proteinen_US
dc.subject.meshChorionic Gonadotropin - Pharmacologyen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Geneticsen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Geneticsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptor Activity-Modifying Protein 1en_US
dc.subject.meshReceptor Activity-Modifying Protein 2en_US
dc.subject.meshReceptor Activity-Modifying Protein 3en_US
dc.subject.meshReceptor Activity-Modifying Proteinsen_US
dc.subject.meshReceptors, Adrenomedullinen_US
dc.subject.meshReceptors, Calcitonin - Genetics - Metabolismen_US
dc.subject.meshReceptors, G-Protein-Coupled - Genetics - Metabolismen_US
dc.subject.meshTestis - Drug Effects - Metabolismen_US
dc.subject.meshTestosterone - Secretionen_US
dc.titleAdrenomedullin peptide: Gene expression of adrenomedullin, its receptors and receptor activity modifying proteins, and receptor binding in rat testis - Actions on testosterone secretionen_HK
dc.typeArticleen_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailLi, YY: cyyli@graduate.hku.hk-
dc.identifier.emailHwang, ISS: isabelbelbel@hotmail.com-
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1095/biolreprod.106.052274en_HK
dc.identifier.pmid16672720-
dc.identifier.scopuseid_2-s2.0-33746318355en_HK
dc.identifier.hkuros117233-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746318355&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue2en_HK
dc.identifier.spage183en_HK
dc.identifier.epage188en_HK
dc.identifier.isiWOS:000239199500004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, YY=49763596700en_HK
dc.identifier.scopusauthoridHwang, ISS=7201615103en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK

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