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Article: Genome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray

TitleGenome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray
Authors
Issue Date2006
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2006, v. 118 n. 10, p. 2461-2469 How to Cite?
AbstractAn analysis of gene expression profiles obtained from cervical cancers was performed to find those genes most aberrantly expressed. Total RNA was prepared from 29 samples of cervical squamous cell carcinoma and 18 control samples, and hybridized to Affymetrix oligonucleotide microarrays with probe sets complementary to over 20,000 transcripts. Unsupervised hierarchical clustering of the expression data readily distinguished normal cervix from cancer. Supervised analysis of gene expression data identified 98 and 139 genes that exhibited >2-fold upregulation and >2-fold down-regulation, respectively, in cervical cancer compared to normal cervix. Several of the genes that were differentially regulated included SPP1 (Osteopontin), CDKN2A (p16), RPL39L, Clorf1, MAL, p11, ARS and NICE-1, These were validated by quantitative RT-PCR on an independent set of cancer and control specimens. Gene Ontology analysis showed that the list of differentially expressed genes included ones that were involved in multiple biological processes, including cell proliferation, cell cycle and protein catabolism. Immunohistochemical staining of cancer specimens further confirmed differential expression of SPP1 in cervical cancer cells vs. nontumor cells. In addition, 2 genes, CTGF and RGS1 were found to be upregulated in late stage cancer compared to early stage cancer, suggesting that they might be involved in cancer progression. The pathway analysis of expression data showed that the SPP1, VEGF, CDC2 and CKS2 genes were coordinately differentially regulated between cancer and normal. The present study is promising and provides potential new insights into the extent of expression differences underlying the development and progression of cervical squamous cell cancer. This study has also revealed several genes that may be highly attractive candidate molecular markers/targets for cervical cancer diagnosis, prognosis and therapy. © 2005 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/149655
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, YFen_US
dc.contributor.authorCheung, THen_US
dc.contributor.authorTsao, GSWen_US
dc.contributor.authorLo, KWKen_US
dc.contributor.authorYim, SFen_US
dc.contributor.authorWang, VWen_US
dc.contributor.authorHeung, MMSen_US
dc.contributor.authorChan, SCSen_US
dc.contributor.authorChan, LKYen_US
dc.contributor.authorHo, TWFen_US
dc.contributor.authorWong, KWYen_US
dc.contributor.authorLi, Cen_US
dc.contributor.authorGuo, Yen_US
dc.contributor.authorChung, TKHen_US
dc.contributor.authorSmith, DIen_US
dc.date.accessioned2012-06-26T05:56:38Z-
dc.date.available2012-06-26T05:56:38Z-
dc.date.issued2006en_US
dc.identifier.citationInternational Journal Of Cancer, 2006, v. 118 n. 10, p. 2461-2469en_US
dc.identifier.issn0020-7136en_US
dc.identifier.urihttp://hdl.handle.net/10722/149655-
dc.description.abstractAn analysis of gene expression profiles obtained from cervical cancers was performed to find those genes most aberrantly expressed. Total RNA was prepared from 29 samples of cervical squamous cell carcinoma and 18 control samples, and hybridized to Affymetrix oligonucleotide microarrays with probe sets complementary to over 20,000 transcripts. Unsupervised hierarchical clustering of the expression data readily distinguished normal cervix from cancer. Supervised analysis of gene expression data identified 98 and 139 genes that exhibited >2-fold upregulation and >2-fold down-regulation, respectively, in cervical cancer compared to normal cervix. Several of the genes that were differentially regulated included SPP1 (Osteopontin), CDKN2A (p16), RPL39L, Clorf1, MAL, p11, ARS and NICE-1, These were validated by quantitative RT-PCR on an independent set of cancer and control specimens. Gene Ontology analysis showed that the list of differentially expressed genes included ones that were involved in multiple biological processes, including cell proliferation, cell cycle and protein catabolism. Immunohistochemical staining of cancer specimens further confirmed differential expression of SPP1 in cervical cancer cells vs. nontumor cells. In addition, 2 genes, CTGF and RGS1 were found to be upregulated in late stage cancer compared to early stage cancer, suggesting that they might be involved in cancer progression. The pathway analysis of expression data showed that the SPP1, VEGF, CDC2 and CKS2 genes were coordinately differentially regulated between cancer and normal. The present study is promising and provides potential new insights into the extent of expression differences underlying the development and progression of cervical squamous cell cancer. This study has also revealed several genes that may be highly attractive candidate molecular markers/targets for cervical cancer diagnosis, prognosis and therapy. © 2005 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_US
dc.relation.ispartofInternational Journal of Canceren_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshDiagnosis, Differentialen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGenes, Neoplasmen_US
dc.subject.meshGenetic Markersen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.subject.meshPrognosisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshUterine Cervical Neoplasms - Diagnosis - Genetics - Therapyen_US
dc.titleGenome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarrayen_US
dc.typeArticleen_US
dc.identifier.emailTsao, GSW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, GSW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/ijc.21660en_US
dc.identifier.pmid16353136-
dc.identifier.scopuseid_2-s2.0-33646448419en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646448419&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume118en_US
dc.identifier.issue10en_US
dc.identifier.spage2461en_US
dc.identifier.epage2469en_US
dc.identifier.isiWOS:000237029200013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, YF=7403041448en_US
dc.identifier.scopusauthoridCheung, TH=7103334169en_US
dc.identifier.scopusauthoridTsao, GSW=7102813116en_US
dc.identifier.scopusauthoridLo, KWK=24302880900en_US
dc.identifier.scopusauthoridYim, SF=7101624278en_US
dc.identifier.scopusauthoridWang, VW=7007164250en_US
dc.identifier.scopusauthoridHeung, MMS=13406236900en_US
dc.identifier.scopusauthoridChan, SCS=36851783200en_US
dc.identifier.scopusauthoridChan, LKY=36907178600en_US
dc.identifier.scopusauthoridHo, TWF=13406972900en_US
dc.identifier.scopusauthoridWong, KWY=25949175200en_US
dc.identifier.scopusauthoridLi, C=7501675575en_US
dc.identifier.scopusauthoridGuo, Y=13406588200en_US
dc.identifier.scopusauthoridChung, TKH=8866217800en_US
dc.identifier.scopusauthoridSmith, DI=7410361498en_US

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