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Article: Microarray analysis of gene expression patterns in adult spinal motoneurons after different types of axonal injuries

TitleMicroarray analysis of gene expression patterns in adult spinal motoneurons after different types of axonal injuries
Authors
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 2006, v. 1075 n. 1, p. 1-12 How to Cite?
AbstractThree experimental models of axonal injuries in adult rat spinal motoneurons were established to investigate changes of gene expression in response to such injuries. We took advantage of cDNA microarray analysis to determine the differential expression of genes in injured motoneurons following distal axotomy or root avulsion in the absence or presence of BDNF. The major finding was that, in response to proximal axonal injury (avulsion), expression of genes that are known to facilitate neuronal survival and axonal regeneration (e.g., IGFRII, PI3K, IGFBP-6, GSTs, GalR2) were down-regulated; but following treatment with BDNF they were up-regulated. In addition, the expression of genes known to be involved in apoptosis and DNA damage (e.g., ANX5, TS, ALR) were down-regulated in BDNF-treated animals with avulsion. Furthermore, many functional families of genes previously shown to play roles in the pathophysiology of axonal injury, including SNAP-25A, SV2B, Ras-related ras3a/4b, ERK1/2, 14-3-3 proteins, proteasome proteins, oncogenes, GAP-43, and NMDAR1, were altered after either distal axotomy or avulsion injury. Some of the changes in gene expression, including Lim-2, FRAG1, GlaR2, GSTs, ALR, TS, ANX3/5, and nhe1/2, are first reported here in injured motoneurons. The differential expression of genes identified by the expression arrays was confirmed by gene-specific RT-PCR for eight genes (GAP-43, IGFR II, Lim-2, MIF, NDAP1, TS, PCC3, and FRAG1) and by in situ hybridization for Lim-2. These results suggest that abnormal regulation of particular biochemical pathways may induce motoneuron death after ventral root avulsion in adult animals. This study presents an approach for selecting specific genes and their products that may be involved in motoneuron degeneration following axonal injuries. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/149652
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Yen_US
dc.contributor.authorXie, Yen_US
dc.contributor.authorChai, Hen_US
dc.contributor.authorFan, Men_US
dc.contributor.authorLiu, Sen_US
dc.contributor.authorLiu, Hen_US
dc.contributor.authorBruce, Ien_US
dc.contributor.authorWu, Wen_US
dc.date.accessioned2012-06-26T05:56:36Z-
dc.date.available2012-06-26T05:56:36Z-
dc.date.issued2006en_US
dc.identifier.citationBrain Research, 2006, v. 1075 n. 1, p. 1-12en_US
dc.identifier.issn0006-8993en_US
dc.identifier.urihttp://hdl.handle.net/10722/149652-
dc.description.abstractThree experimental models of axonal injuries in adult rat spinal motoneurons were established to investigate changes of gene expression in response to such injuries. We took advantage of cDNA microarray analysis to determine the differential expression of genes in injured motoneurons following distal axotomy or root avulsion in the absence or presence of BDNF. The major finding was that, in response to proximal axonal injury (avulsion), expression of genes that are known to facilitate neuronal survival and axonal regeneration (e.g., IGFRII, PI3K, IGFBP-6, GSTs, GalR2) were down-regulated; but following treatment with BDNF they were up-regulated. In addition, the expression of genes known to be involved in apoptosis and DNA damage (e.g., ANX5, TS, ALR) were down-regulated in BDNF-treated animals with avulsion. Furthermore, many functional families of genes previously shown to play roles in the pathophysiology of axonal injury, including SNAP-25A, SV2B, Ras-related ras3a/4b, ERK1/2, 14-3-3 proteins, proteasome proteins, oncogenes, GAP-43, and NMDAR1, were altered after either distal axotomy or avulsion injury. Some of the changes in gene expression, including Lim-2, FRAG1, GlaR2, GSTs, ALR, TS, ANX3/5, and nhe1/2, are first reported here in injured motoneurons. The differential expression of genes identified by the expression arrays was confirmed by gene-specific RT-PCR for eight genes (GAP-43, IGFR II, Lim-2, MIF, NDAP1, TS, PCC3, and FRAG1) and by in situ hybridization for Lim-2. These results suggest that abnormal regulation of particular biochemical pathways may induce motoneuron death after ventral root avulsion in adult animals. This study presents an approach for selecting specific genes and their products that may be involved in motoneuron degeneration following axonal injuries. © 2005 Elsevier B.V. All rights reserved.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_US
dc.relation.ispartofBrain Researchen_US
dc.subject.meshAnimalsen_US
dc.subject.meshDna Primersen_US
dc.subject.meshDiffuse Axonal Injury - Geneticsen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMotor Neurons - Physiologyen_US
dc.subject.meshNerve Tissue Proteins - Geneticsen_US
dc.subject.meshOligonucleotide Array Sequence Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSpinal Cord - Physiopathologyen_US
dc.subject.meshSpinal Cord Injuries - Geneticsen_US
dc.titleMicroarray analysis of gene expression patterns in adult spinal motoneurons after different types of axonal injuriesen_US
dc.typeArticleen_US
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.brainres.2005.12.060en_US
dc.identifier.pmid16460709-
dc.identifier.scopuseid_2-s2.0-33644904656en_US
dc.identifier.hkuros121281-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644904656&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1075en_US
dc.identifier.issue1en_US
dc.identifier.spage1en_US
dc.identifier.epage12en_US
dc.identifier.isiWOS:000236564700001-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridYang, Y=8675011000en_US
dc.identifier.scopusauthoridXie, Y=12778428300en_US
dc.identifier.scopusauthoridChai, H=35918658800en_US
dc.identifier.scopusauthoridFan, M=7201970880en_US
dc.identifier.scopusauthoridLiu, S=8597874800en_US
dc.identifier.scopusauthoridLiu, H=36071557800en_US
dc.identifier.scopusauthoridBruce, I=35612490700en_US
dc.identifier.scopusauthoridWu, W=7407081122en_US
dc.identifier.citeulike1933314-

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