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Article: Immune modulatory effects of Prunella vulgaris L. on monocytes/macrophages.

TitleImmune modulatory effects of Prunella vulgaris L. on monocytes/macrophages.
Authors
Issue Date2005
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine., 2005, v. 16 n. 6, p. 1109-1116 How to Cite?
AbstractPrunella vulgaris L. (Labiatae), a popular Western and Chinese herbal medicine, has long been associated with anti-viral and anti-bacterial effects. While its anti-viral effects are attributed mainly to the inhibition of virus replication, the biological mechanisms of its anti-bacterial effects remain unknown. As a biological response modifier (BRM), the polysaccharides isolated from P. vulgaris have been shown to up-regulate the immune responses of monocytes/macrophages. However, the immune stimulatory effects seem to contradict its well-known anti-inflammatory properties. We hypothesized that the anti-microbial effects exhibited by the polysaccharides isolated from P. vulgaris encompass both anti-inflammatory and immune stimulatory effects. One of the polysaccharide fractions PV2IV markedly stimulated the production of superoxide and nitrite representing nitric oxide from murine macrophage RAW264.7 and brain macrophage BV2 cells. The amount of nitrite and superoxide produced after PV2IV stimulation was as high as that stimulated by bacterial endotoxin lipopolysaccharide (LPS) in a dose-dependent manner. In addition, PV2IV also increased cellular protein levels of inducible nitric oxide synthase (iNOS) and mRNA for tumor necrosis factor-alpha (TNFalpha). Similar to the effects of a high dose of LPS, the fraction PV2 could trigger activation-induced cell death (AICD) by stimulating caspase-3 activity and reduction of MTT uptake in monocytes/macrophages. These results may help our understanding of the molecular mechanism of P. vulgaris, which exhibited both immune stimulatory and anti-inflammatory effects against microbial invasion.
Persistent Identifierhttp://hdl.handle.net/10722/149651
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFang, Xen_US
dc.contributor.authorYu, MMen_US
dc.contributor.authorYuen, WHen_US
dc.contributor.authorZee, SYen_US
dc.contributor.authorChang, RCen_US
dc.date.accessioned2012-06-26T05:56:34Z-
dc.date.available2012-06-26T05:56:34Z-
dc.date.issued2005en_US
dc.identifier.citationInternational Journal Of Molecular Medicine., 2005, v. 16 n. 6, p. 1109-1116en_US
dc.identifier.issn1107-3756en_US
dc.identifier.urihttp://hdl.handle.net/10722/149651-
dc.description.abstractPrunella vulgaris L. (Labiatae), a popular Western and Chinese herbal medicine, has long been associated with anti-viral and anti-bacterial effects. While its anti-viral effects are attributed mainly to the inhibition of virus replication, the biological mechanisms of its anti-bacterial effects remain unknown. As a biological response modifier (BRM), the polysaccharides isolated from P. vulgaris have been shown to up-regulate the immune responses of monocytes/macrophages. However, the immune stimulatory effects seem to contradict its well-known anti-inflammatory properties. We hypothesized that the anti-microbial effects exhibited by the polysaccharides isolated from P. vulgaris encompass both anti-inflammatory and immune stimulatory effects. One of the polysaccharide fractions PV2IV markedly stimulated the production of superoxide and nitrite representing nitric oxide from murine macrophage RAW264.7 and brain macrophage BV2 cells. The amount of nitrite and superoxide produced after PV2IV stimulation was as high as that stimulated by bacterial endotoxin lipopolysaccharide (LPS) in a dose-dependent manner. In addition, PV2IV also increased cellular protein levels of inducible nitric oxide synthase (iNOS) and mRNA for tumor necrosis factor-alpha (TNFalpha). Similar to the effects of a high dose of LPS, the fraction PV2 could trigger activation-induced cell death (AICD) by stimulating caspase-3 activity and reduction of MTT uptake in monocytes/macrophages. These results may help our understanding of the molecular mechanism of P. vulgaris, which exhibited both immune stimulatory and anti-inflammatory effects against microbial invasion.en_US
dc.languageengen_US
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_US
dc.relation.ispartofInternational journal of molecular medicine.en_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain - Metabolismen_US
dc.subject.meshCaspase 3en_US
dc.subject.meshCaspases - Metabolismen_US
dc.subject.meshCell Death - Drug Effectsen_US
dc.subject.meshDrugs, Chinese Herbal - Chemistry - Pharmacologyen_US
dc.subject.meshFormazans - Pharmacologyen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshImmune System - Drug Effectsen_US
dc.subject.meshMacrophages - Drug Effects - Immunologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMonocytes - Drug Effects - Immunologyen_US
dc.subject.meshNitric Oxide Synthase Type Ii - Metabolismen_US
dc.subject.meshNitrites - Metabolismen_US
dc.subject.meshPrunellaen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshSuperoxides - Metabolismen_US
dc.subject.meshTetrazolium Salts - Pharmacologyen_US
dc.subject.meshTumor Necrosis Factor-Alpha - Metabolismen_US
dc.titleImmune modulatory effects of Prunella vulgaris L. on monocytes/macrophages.en_US
dc.typeArticleen_US
dc.identifier.emailChang, RC:rccchang@hkucc.hku.hken_US
dc.identifier.authorityChang, RC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16273294-
dc.identifier.scopuseid_2-s2.0-33644876604en_US
dc.identifier.hkuros113053-
dc.identifier.volume16en_US
dc.identifier.issue6en_US
dc.identifier.spage1109en_US
dc.identifier.epage1116en_US
dc.identifier.isiWOS:000233575300021-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridFang, X=12778042000en_US
dc.identifier.scopusauthoridYu, MM=36800579400en_US
dc.identifier.scopusauthoridYuen, WH=7102761282en_US
dc.identifier.scopusauthoridZee, SY=7004001733en_US
dc.identifier.scopusauthoridChang, RC=7403713410en_US

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