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Article: Survival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion

TitleSurvival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion
Authors
Issue Date2004
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJN
Citation
European Journal Of Neuroscience, 2004, v. 19 n. 8, p. 2123-2131 How to Cite?
AbstractWe investigated the functional recovery of motoneurons after reimplanting an avulsed ventral root in a rat model of traction injury. The eighth cervical root (C8) was avulsed by controlled traction and immediately reimplanted to the spinal cord. Spinal nerves from neighbouring segments (C5, C6, C7 and T1) were ligated and cut. After 12 or 20 weeks, the survival, regeneration and functional recovery of spinal motoneurons were evaluated by Nissl staining, retrograde labelling of motoneurons, NOS histochemistry, histological examination of muscle and nerve-muscle junction, electromyography and behavioural observation. In the control animals, about 14% or 11% of spinal motoneurons survived 12 or 20 weeks postinjury, respectively. By contrast, in animals with ventral root reimplantation, 62% and 55% of motoneurons survived at 12 or 20 weeks postinjury, respectively. Retrograde labelling and histological examination indicated that about 90% of the surviving motoneurons in the C8 segment regenerated axons into the reimplanted ventral root. Staining the muscles with silver and cholinesterase revealed new motor endplates in the reinnervated muscle. Functionally significant electromyographic responses in flexor digitorum superficial and flexor carpi radialis were observed in experimental animals; however, the average latency of the motor action potentials was greater than normal control. The grasping test showed functional recovery of finger flexors and median nerve. In conclusion, our results indicate that spinal motoneurons can regenerate axons through reimplanted roots and reinnervate muscles to recover partial function.
Persistent Identifierhttp://hdl.handle.net/10722/149639
ISSN
2015 Impact Factor: 2.975
2015 SCImago Journal Rankings: 2.130
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGu, HYen_US
dc.contributor.authorChai, Hen_US
dc.contributor.authorZhang, JYen_US
dc.contributor.authorYao, ZBen_US
dc.contributor.authorZhou, LHen_US
dc.contributor.authorWong, WMen_US
dc.contributor.authorBruce, Ien_US
dc.contributor.authorWu, WTen_US
dc.date.accessioned2012-06-26T05:56:22Z-
dc.date.available2012-06-26T05:56:22Z-
dc.date.issued2004en_US
dc.identifier.citationEuropean Journal Of Neuroscience, 2004, v. 19 n. 8, p. 2123-2131en_US
dc.identifier.issn0953-816Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/149639-
dc.description.abstractWe investigated the functional recovery of motoneurons after reimplanting an avulsed ventral root in a rat model of traction injury. The eighth cervical root (C8) was avulsed by controlled traction and immediately reimplanted to the spinal cord. Spinal nerves from neighbouring segments (C5, C6, C7 and T1) were ligated and cut. After 12 or 20 weeks, the survival, regeneration and functional recovery of spinal motoneurons were evaluated by Nissl staining, retrograde labelling of motoneurons, NOS histochemistry, histological examination of muscle and nerve-muscle junction, electromyography and behavioural observation. In the control animals, about 14% or 11% of spinal motoneurons survived 12 or 20 weeks postinjury, respectively. By contrast, in animals with ventral root reimplantation, 62% and 55% of motoneurons survived at 12 or 20 weeks postinjury, respectively. Retrograde labelling and histological examination indicated that about 90% of the surviving motoneurons in the C8 segment regenerated axons into the reimplanted ventral root. Staining the muscles with silver and cholinesterase revealed new motor endplates in the reinnervated muscle. Functionally significant electromyographic responses in flexor digitorum superficial and flexor carpi radialis were observed in experimental animals; however, the average latency of the motor action potentials was greater than normal control. The grasping test showed functional recovery of finger flexors and median nerve. In conclusion, our results indicate that spinal motoneurons can regenerate axons through reimplanted roots and reinnervate muscles to recover partial function.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJNen_US
dc.relation.ispartofEuropean Journal of Neuroscienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Survival - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMotor Neurons - Pathology - Physiologyen_US
dc.subject.meshNerve Regeneration - Physiologyen_US
dc.subject.meshRadiculopathy - Pathology - Surgeryen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReplantation - Methodsen_US
dc.subject.meshSpinal Nerve Roots - Cytology - Physiology - Surgeryen_US
dc.titleSurvival, regeneration and functional recovery of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsionen_US
dc.typeArticleen_US
dc.identifier.emailWu, WT:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, WT=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.0953-816X.2004.03295.xen_US
dc.identifier.pmid15090039-
dc.identifier.scopuseid_2-s2.0-2342614777en_US
dc.identifier.hkuros88056-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2342614777&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume19en_US
dc.identifier.issue8en_US
dc.identifier.spage2123en_US
dc.identifier.epage2131en_US
dc.identifier.isiWOS:000221197600012-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridGu, HY=11339561900en_US
dc.identifier.scopusauthoridChai, H=35918658800en_US
dc.identifier.scopusauthoridZhang, JY=35492256700en_US
dc.identifier.scopusauthoridYao, ZB=7401467890en_US
dc.identifier.scopusauthoridZhou, LH=7404125592en_US
dc.identifier.scopusauthoridWong, WM=7403972413en_US
dc.identifier.scopusauthoridBruce, I=35612490700en_US
dc.identifier.scopusauthoridWu, WT=7407081122en_US

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