File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects.

TitleCharacterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects.
Authors
Issue Date2004
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2004, v. 14 n. 5, p. 917-924 How to Cite?
AbstractDegeneration of neurons is a key problem in Alzheimer's disease (AD) and neuroprotection is a possible way to safeguard neurons from neurodegeneration. Polysaccharides isolated from Chinese medicinal herbs have been investigated extensively for their anti-tumor and immune stimulating effects. Yet, little is known about the effects of polysaccharides in neurons. Recently, two pure polysaccharides isolated from the flowers of Nerium indicum were shown to stimulate proliferation and differentiation of PC12 pheochromocytoma cells, an effect similar to that observed from nerve growth factor. In this notion, it is hypothesized that polysaccharides isolated from the flowers of N. indicum could exhibit beneficial effects in neurons. In this study, we isolated, characterized and investigated two new polysaccharides from the flowers of N. indicum for their neuroprotective effects on neurons against serum-deprivation and beta-amyloid (Abeta) peptide toxicity in primary rat cortical neuronal cultures. Pretreatment of the polysaccharides significantly reduced the number of apoptotic neurons revealed by DAPI staining when neurons were exposed to serum-free medium. Besides, the polysaccharides could also decrease the activity of caspase-3 triggered by Abeta peptides. Western blot analysis indicated that polysaccharides stimulated the phosphorylation of PDK-1 (Serine 241) and Akt (Threonine 308). In conclusion, the polysaccharides J2, J3 and J4 isolated from N. indicum provide a lead for future development of neuroprotective agent against neuronal death in neurodegenerative diseases and the neuroprotective mechanism may primarily rely on activation of Akt survival signaling pathway.
Persistent Identifierhttp://hdl.handle.net/10722/149638
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYu, MSen_US
dc.contributor.authorLai, SWen_US
dc.contributor.authorLin, KFen_US
dc.contributor.authorFang, JNen_US
dc.contributor.authorYuen, WHen_US
dc.contributor.authorChang, RCen_US
dc.date.accessioned2012-06-26T05:56:22Z-
dc.date.available2012-06-26T05:56:22Z-
dc.date.issued2004en_US
dc.identifier.citationInternational Journal Of Molecular Medicine, 2004, v. 14 n. 5, p. 917-924en_US
dc.identifier.issn1107-3756en_US
dc.identifier.urihttp://hdl.handle.net/10722/149638-
dc.description.abstractDegeneration of neurons is a key problem in Alzheimer's disease (AD) and neuroprotection is a possible way to safeguard neurons from neurodegeneration. Polysaccharides isolated from Chinese medicinal herbs have been investigated extensively for their anti-tumor and immune stimulating effects. Yet, little is known about the effects of polysaccharides in neurons. Recently, two pure polysaccharides isolated from the flowers of Nerium indicum were shown to stimulate proliferation and differentiation of PC12 pheochromocytoma cells, an effect similar to that observed from nerve growth factor. In this notion, it is hypothesized that polysaccharides isolated from the flowers of N. indicum could exhibit beneficial effects in neurons. In this study, we isolated, characterized and investigated two new polysaccharides from the flowers of N. indicum for their neuroprotective effects on neurons against serum-deprivation and beta-amyloid (Abeta) peptide toxicity in primary rat cortical neuronal cultures. Pretreatment of the polysaccharides significantly reduced the number of apoptotic neurons revealed by DAPI staining when neurons were exposed to serum-free medium. Besides, the polysaccharides could also decrease the activity of caspase-3 triggered by Abeta peptides. Western blot analysis indicated that polysaccharides stimulated the phosphorylation of PDK-1 (Serine 241) and Akt (Threonine 308). In conclusion, the polysaccharides J2, J3 and J4 isolated from N. indicum provide a lead for future development of neuroprotective agent against neuronal death in neurodegenerative diseases and the neuroprotective mechanism may primarily rely on activation of Akt survival signaling pathway.en_US
dc.languageengen_US
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_US
dc.relation.ispartofInternational journal of molecular medicineen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCerebral Cortexen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshFlowers - Chemistryen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshNerium - Chemistryen_US
dc.subject.meshNerve Degeneration - Prevention & Controlen_US
dc.subject.meshNeurons - Cytology - Drug Effectsen_US
dc.subject.meshPhytotherapyen_US
dc.subject.meshPolysaccharides - Chemistry - Isolation & Purification - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.titleCharacterization of polysaccharides from the flowers of Nerium indicum and their neuroprotective effects.en_US
dc.typeArticleen_US
dc.identifier.emailChang, RC:rccchang@hkucc.hku.hken_US
dc.identifier.authorityChang, RC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid15492866-
dc.identifier.scopuseid_2-s2.0-21644449760en_US
dc.identifier.hkuros148363-
dc.identifier.volume14en_US
dc.identifier.issue5en_US
dc.identifier.spage917en_US
dc.identifier.epage924en_US
dc.identifier.isiWOS:000224737700022-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridYu, MS=35346047600en_US
dc.identifier.scopusauthoridLai, SW=8729823700en_US
dc.identifier.scopusauthoridLin, KF=8744549500en_US
dc.identifier.scopusauthoridFang, JN=7402963759en_US
dc.identifier.scopusauthoridYuen, WH=7102761282en_US
dc.identifier.scopusauthoridChang, RC=7403713410en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats