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Article: Soluble fibrinogen-like protein 2/fibroleukin exhibits immunosuppressive properties: Suppressing T cell proliferation and inhibiting maturation of bone marrow-derived dendritic cells

TitleSoluble fibrinogen-like protein 2/fibroleukin exhibits immunosuppressive properties: Suppressing T cell proliferation and inhibiting maturation of bone marrow-derived dendritic cells
Authors
Issue Date2003
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal Of Immunology, 2003, v. 170 n. 8, p. 4036-4044 How to Cite?
AbstractFibrinogen-like protein 2 (fgl2)/fibroleukin is a member of the fibrinogen-related protein superfamily. In addition to its established role in triggering thrombosis, it is known to be secreted by T cells. The soluble fgl2 (sfgl2) protein generated in a baculovirus expression system bound to both T cells and bone marrow-derived dendritic cells (DC) in a specific manner. sfgl2 exhibited immunomodulatory properties capable of inhibiting T cell proliferation stimulated by alloantigens, anti-CD3/anti-CD28 mAbs, and Con A in a dose-dependent manner; however, it had no inhibitory effects on CTL activity. The time- and dose-dependent inhibitory effect of sfgl2 on alloreactive T cell proliferation could be neutralized by a mAb against mouse fgl2. Polarization toward a Th2 cytokine profile with decreased production of IL-2 and IFN-γ and increased production of IL-4 and IL-10 was observed in sfgl2-treated allogeneic cultures. Exposure of immature DC to sfgl2 abrogated the expression of CD80high and MHC class IIhigh molecules and markedly inhibited NF-κB nuclear translocation, thus inhibiting their maturation. sFgl2-treated DC had an impaired ability to stimulate allogeneic T cell proliferation. Maximal inhibition of proliferation was observed when allogeneic T cells were cultured with sfgl2-treated DC and sfgl2 protein was added in the culture. These data provide the first evidence to demonstrate that sfgl2 exerts immunosuppressive effects on T cell proliferation and DC maturation.
Persistent Identifierhttp://hdl.handle.net/10722/149614
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, CWYen_US
dc.contributor.authorKay, LSen_US
dc.contributor.authorKhadaroo, RGen_US
dc.contributor.authorChan, MWCen_US
dc.contributor.authorLakatoo, Sen_US
dc.contributor.authorYoung, KJen_US
dc.contributor.authorZhang, Len_US
dc.contributor.authorGorczynski, RMen_US
dc.contributor.authorCattral, Men_US
dc.contributor.authorRotstein, Oen_US
dc.contributor.authorLevy, GAen_US
dc.date.accessioned2012-06-26T05:56:02Z-
dc.date.available2012-06-26T05:56:02Z-
dc.date.issued2003en_US
dc.identifier.citationJournal Of Immunology, 2003, v. 170 n. 8, p. 4036-4044en_US
dc.identifier.issn0022-1767en_US
dc.identifier.urihttp://hdl.handle.net/10722/149614-
dc.description.abstractFibrinogen-like protein 2 (fgl2)/fibroleukin is a member of the fibrinogen-related protein superfamily. In addition to its established role in triggering thrombosis, it is known to be secreted by T cells. The soluble fgl2 (sfgl2) protein generated in a baculovirus expression system bound to both T cells and bone marrow-derived dendritic cells (DC) in a specific manner. sfgl2 exhibited immunomodulatory properties capable of inhibiting T cell proliferation stimulated by alloantigens, anti-CD3/anti-CD28 mAbs, and Con A in a dose-dependent manner; however, it had no inhibitory effects on CTL activity. The time- and dose-dependent inhibitory effect of sfgl2 on alloreactive T cell proliferation could be neutralized by a mAb against mouse fgl2. Polarization toward a Th2 cytokine profile with decreased production of IL-2 and IFN-γ and increased production of IL-4 and IL-10 was observed in sfgl2-treated allogeneic cultures. Exposure of immature DC to sfgl2 abrogated the expression of CD80high and MHC class IIhigh molecules and markedly inhibited NF-κB nuclear translocation, thus inhibiting their maturation. sFgl2-treated DC had an impaired ability to stimulate allogeneic T cell proliferation. Maximal inhibition of proliferation was observed when allogeneic T cells were cultured with sfgl2-treated DC and sfgl2 protein was added in the culture. These data provide the first evidence to demonstrate that sfgl2 exerts immunosuppressive effects on T cell proliferation and DC maturation.en_US
dc.languageengen_US
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_US
dc.relation.ispartofJournal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd80 - Biosynthesisen_US
dc.subject.meshApoptosis - Immunologyen_US
dc.subject.meshBaculoviridae - Geneticsen_US
dc.subject.meshBone Marrow Cells - Cytology - Immunology - Metabolismen_US
dc.subject.meshCell Differentiation - Immunologyen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCytokines - Biosynthesisen_US
dc.subject.meshDendritic Cells - Cytology - Immunology - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFibrinogen - Biosynthesis - Genetics - Metabolism - Physiologyen_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshGrowth Inhibitors - Biosynthesis - Genetics - Metabolism - Physiologyen_US
dc.subject.meshHistocompatibility Antigens Class Ii - Biosynthesisen_US
dc.subject.meshLymphocyte Activation - Immunologyen_US
dc.subject.meshLymphocyte Culture Test, Mixeden_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Aen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshProtein Binding - Immunologyen_US
dc.subject.meshSolubilityen_US
dc.subject.meshSuppressor Factors, Immunologic - Biosynthesis - Genetics - Metabolism - Physiologyen_US
dc.subject.meshT-Lymphocytes - Cytology - Immunology - Metabolismen_US
dc.subject.meshTh2 Cells - Immunology - Metabolismen_US
dc.subject.meshThromboplastin - Biosynthesis - Genetics - Metabolism - Physiologyen_US
dc.titleSoluble fibrinogen-like protein 2/fibroleukin exhibits immunosuppressive properties: Suppressing T cell proliferation and inhibiting maturation of bone marrow-derived dendritic cellsen_US
dc.typeArticleen_US
dc.identifier.emailChan, CWY:camchan@hku.hken_US
dc.identifier.authorityChan, CWY=rp01311en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid12682232-
dc.identifier.scopuseid_2-s2.0-0037446535en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037446535&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume170en_US
dc.identifier.issue8en_US
dc.identifier.spage4036en_US
dc.identifier.epage4044en_US
dc.identifier.isiWOS:000182171100013-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, CWY=12240386600en_US
dc.identifier.scopusauthoridKay, LS=8785107100en_US
dc.identifier.scopusauthoridKhadaroo, RG=6602704880en_US
dc.identifier.scopusauthoridChan, MWC=36941295800en_US
dc.identifier.scopusauthoridLakatoo, S=6508044811en_US
dc.identifier.scopusauthoridYoung, KJ=7402600857en_US
dc.identifier.scopusauthoridZhang, L=23156618200en_US
dc.identifier.scopusauthoridGorczynski, RM=35431895200en_US
dc.identifier.scopusauthoridCattral, M=7005041726en_US
dc.identifier.scopusauthoridRotstein, O=35452077300en_US
dc.identifier.scopusauthoridLevy, GA=35391580500en_US

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