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Article: Effects of erythromycin on Pseudomonas aeruginosa adherence to collagen and morphology in vitro

TitleEffects of erythromycin on Pseudomonas aeruginosa adherence to collagen and morphology in vitro
Authors
KeywordsBacterial adherence
Bacterial morphology
Erythromycin
Pseudomonas aeruginosa
Subminimal inhibitory concentration
Issue Date2003
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
Citation
European Respiratory Journal, 2003, v. 21 n. 3, p. 401-406 How to Cite?
AbstractThe airways of patients with bronchiectasis and cystic fibrosis are often chronically colonised by Pseudomonas aeruginosa (PA), which is virtually impossible to eradicate. Low-dose erythromycin (EM), for unknown mechanisms, is efficacious in bronchiectasis and diffuse panbronchiolitis. In this study, an in vitro model to investigate PA adherence to human type IV basement collagen was developed by using scanning electron microscopy (SEM). There were significantly less PA bacilli per 20 random SEM fields (4,000x) when PA was cultured in 0.05, 0.5 and 5 μg·mL -1 of EM compared with control (absence of EM). Adherence density (20 SEM fields·log -1 inocular size) for PA obtained from no EM (56.8±43.16) was significantly higher than that obtained from 0.05, 0.5, and 5 μg·mL -1 EM (21.5±17.56, 23.3±16.65, and 21.4±12.65 respectively). By using SEM it was found that PA, when incubated in EM (0.05, 0.5, 5 μg·mL -1) had a significant reduction in its diagonal length, radius, height, volume and surface area. It is possible, therefore, that these misshaped Pseudomonas aeruginosa bacilli are more susceptible to host defence mechanisms, while at the same time less adherent to the basement membrane of the airway in vivo. Therefore, this could help explain the clinical efficacy of low-dose erythromycin therapy on patients with Pseudomonas aeruginosa infection.
Persistent Identifierhttp://hdl.handle.net/10722/149613
ISSN
2015 Impact Factor: 8.332
2015 SCImago Journal Rankings: 3.204
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTsang, KWen_HK
dc.contributor.authorNg, Pen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorChan, Sen_HK
dc.contributor.authorTipoe, Gen_HK
dc.contributor.authorLeung, Ren_HK
dc.contributor.authorSun, Jen_HK
dc.contributor.authorHo, JCen_HK
dc.contributor.authorIp, MSen_HK
dc.contributor.authorLam, WKen_HK
dc.date.accessioned2012-06-26T05:56:01Z-
dc.date.available2012-06-26T05:56:01Z-
dc.date.issued2003en_HK
dc.identifier.citationEuropean Respiratory Journal, 2003, v. 21 n. 3, p. 401-406en_HK
dc.identifier.issn0903-1936en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149613-
dc.description.abstractThe airways of patients with bronchiectasis and cystic fibrosis are often chronically colonised by Pseudomonas aeruginosa (PA), which is virtually impossible to eradicate. Low-dose erythromycin (EM), for unknown mechanisms, is efficacious in bronchiectasis and diffuse panbronchiolitis. In this study, an in vitro model to investigate PA adherence to human type IV basement collagen was developed by using scanning electron microscopy (SEM). There were significantly less PA bacilli per 20 random SEM fields (4,000x) when PA was cultured in 0.05, 0.5 and 5 μg·mL -1 of EM compared with control (absence of EM). Adherence density (20 SEM fields·log -1 inocular size) for PA obtained from no EM (56.8±43.16) was significantly higher than that obtained from 0.05, 0.5, and 5 μg·mL -1 EM (21.5±17.56, 23.3±16.65, and 21.4±12.65 respectively). By using SEM it was found that PA, when incubated in EM (0.05, 0.5, 5 μg·mL -1) had a significant reduction in its diagonal length, radius, height, volume and surface area. It is possible, therefore, that these misshaped Pseudomonas aeruginosa bacilli are more susceptible to host defence mechanisms, while at the same time less adherent to the basement membrane of the airway in vivo. Therefore, this could help explain the clinical efficacy of low-dose erythromycin therapy on patients with Pseudomonas aeruginosa infection.en_HK
dc.languageengen_US
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.comen_HK
dc.relation.ispartofEuropean Respiratory Journalen_HK
dc.subjectBacterial adherenceen_HK
dc.subjectBacterial morphologyen_HK
dc.subjectErythromycinen_HK
dc.subjectPseudomonas aeruginosaen_HK
dc.subjectSubminimal inhibitory concentrationen_HK
dc.subject.meshBacterial Adhesion - Drug Effectsen_US
dc.subject.meshBiological Availabilityen_US
dc.subject.meshCollagen - Physiologyen_US
dc.subject.meshCulture Media, Conditioneden_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshErythromycin - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshProbabilityen_US
dc.subject.meshPseudomonas Aeruginosa - Drug Effects - Physiologyen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.titleEffects of erythromycin on Pseudomonas aeruginosa adherence to collagen and morphology in vitroen_HK
dc.typeArticleen_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailTipoe, G:tgeorge@hkucc.hku.hken_HK
dc.identifier.emailHo, JC:jhocm@hku.hken_HK
dc.identifier.emailIp, MS:msmip@hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityTipoe, G=rp00371en_HK
dc.identifier.authorityHo, JC=rp00258en_HK
dc.identifier.authorityIp, MS=rp00347en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid12661992-
dc.identifier.scopuseid_2-s2.0-0037341995en_HK
dc.identifier.hkuros79046-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037341995&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue3en_HK
dc.identifier.spage401en_HK
dc.identifier.epage406en_HK
dc.identifier.isiWOS:000181578200005-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridTsang, KW=7201555024en_HK
dc.identifier.scopusauthoridNg, P=55107639000en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridChan, S=14055434000en_HK
dc.identifier.scopusauthoridTipoe, G=7003550610en_HK
dc.identifier.scopusauthoridLeung, R=7101876102en_HK
dc.identifier.scopusauthoridSun, J=36823256300en_HK
dc.identifier.scopusauthoridHo, JC=7402649981en_HK
dc.identifier.scopusauthoridIp, MS=7102423259en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK

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