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Article: Overexpression of matrix metalloproteinase-8 and -9 in bronchiectatic airways in vivo
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TitleOverexpression of matrix metalloproteinase-8 and -9 in bronchiectatic airways in vivo
 
AuthorsZheng, L1
Lam, WK1
Tipoe, GL1
Shum, IH1
Yan, C1
Leung, R1
Sun, J1
Ooi, GC1
Tsang, KW1
 
KeywordsBronchial Biopsy
Bronchiectasis
Matrix Metalloproteinase
Neutrophils
 
Issue Date2002
 
PublisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
 
CitationEuropean Respiratory Journal, 2002, v. 20 n. 1, p. 170-176 [How to Cite?]
DOI: http://dx.doi.org/10.1183/09031936.02.00282402
 
AbstractThe progressive bronchial dilatation in bronchiectasis is likely to be the result of continued airway matrix destruction, although little is known about the role of neutrophil matrix metalloproteinases (MMPs) in this process. Immunohistochemistry has been used to investigate the expression and cellular localisation of MMP-8 and MMP-9 in bronchiectatic airways in vivo. Endobronchial biopsies were taken from 25 bronchiectatic patients, and from the right lower lobe in 14 control subjects. MMP-8, MMP-9, neutrophils and macrophages were stained with monoclonal antibodies and quantified as positive cell·mm -2 of the lamina propria by using an image analysis system. There were significantly higher densities of MMP-8 and MMP-9 positive cells in the lamina propria of bronchiectatic than control airways. In bronchiectatic airways, the densities of MMP-8 and MMP-9 positive cells correlated with each other and with neutrophil density, but not with macrophage density. In control airways, a significant correlation was found between MMP-8 with neutrophil and MMP-9 with macrophage densities. An overexpression of neutrophil matrix metalloproteinases in bronchiectatic airways could help explain the continuation of airway destruction in bronchiectasis. In view of the clinical availability of matrix metalloproteinase antagonists, the results presented here could have a significant impact on the development of novel therapies of this untreatable disease.
 
ISSN0903-1936
2013 Impact Factor: 7.125
 
DOIhttp://dx.doi.org/10.1183/09031936.02.00282402
 
ISI Accession Number IDWOS:000177188600028
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZheng, L
 
dc.contributor.authorLam, WK
 
dc.contributor.authorTipoe, GL
 
dc.contributor.authorShum, IH
 
dc.contributor.authorYan, C
 
dc.contributor.authorLeung, R
 
dc.contributor.authorSun, J
 
dc.contributor.authorOoi, GC
 
dc.contributor.authorTsang, KW
 
dc.date.accessioned2012-06-26T05:55:51Z
 
dc.date.available2012-06-26T05:55:51Z
 
dc.date.issued2002
 
dc.description.abstractThe progressive bronchial dilatation in bronchiectasis is likely to be the result of continued airway matrix destruction, although little is known about the role of neutrophil matrix metalloproteinases (MMPs) in this process. Immunohistochemistry has been used to investigate the expression and cellular localisation of MMP-8 and MMP-9 in bronchiectatic airways in vivo. Endobronchial biopsies were taken from 25 bronchiectatic patients, and from the right lower lobe in 14 control subjects. MMP-8, MMP-9, neutrophils and macrophages were stained with monoclonal antibodies and quantified as positive cell·mm -2 of the lamina propria by using an image analysis system. There were significantly higher densities of MMP-8 and MMP-9 positive cells in the lamina propria of bronchiectatic than control airways. In bronchiectatic airways, the densities of MMP-8 and MMP-9 positive cells correlated with each other and with neutrophil density, but not with macrophage density. In control airways, a significant correlation was found between MMP-8 with neutrophil and MMP-9 with macrophage densities. An overexpression of neutrophil matrix metalloproteinases in bronchiectatic airways could help explain the continuation of airway destruction in bronchiectasis. In view of the clinical availability of matrix metalloproteinase antagonists, the results presented here could have a significant impact on the development of novel therapies of this untreatable disease.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationEuropean Respiratory Journal, 2002, v. 20 n. 1, p. 170-176 [How to Cite?]
DOI: http://dx.doi.org/10.1183/09031936.02.00282402
 
dc.identifier.doihttp://dx.doi.org/10.1183/09031936.02.00282402
 
dc.identifier.epage176
 
dc.identifier.isiWOS:000177188600028
 
dc.identifier.issn0903-1936
2013 Impact Factor: 7.125
 
dc.identifier.issue1
 
dc.identifier.pmid12166566
 
dc.identifier.scopuseid_2-s2.0-0035989381
 
dc.identifier.spage170
 
dc.identifier.urihttp://hdl.handle.net/10722/149605
 
dc.identifier.volume20
 
dc.languageeng
 
dc.publisherEuropean Respiratory Society. The Journal's web site is located at http://erj.ersjournals.com
 
dc.publisher.placeSwitzerland
 
dc.relation.ispartofEuropean Respiratory Journal
 
dc.relation.referencesReferences in Scopus
 
dc.subjectBronchial Biopsy
 
dc.subjectBronchiectasis
 
dc.subjectMatrix Metalloproteinase
 
dc.subjectNeutrophils
 
dc.titleOverexpression of matrix metalloproteinase-8 and -9 in bronchiectatic airways in vivo
 
dc.typeArticle
 
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<contributor.author>Leung, R</contributor.author>
<contributor.author>Sun, J</contributor.author>
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<description.abstract>The progressive bronchial dilatation in bronchiectasis is likely to be the result of continued airway matrix destruction, although little is known about the role of neutrophil matrix metalloproteinases (MMPs) in this process. Immunohistochemistry has been used to investigate the expression and cellular localisation of MMP-8 and MMP-9 in bronchiectatic airways in vivo. Endobronchial biopsies were taken from 25 bronchiectatic patients, and from the right lower lobe in 14 control subjects. MMP-8, MMP-9, neutrophils and macrophages were stained with monoclonal antibodies and quantified as positive cell&#183;mm -2 of the lamina propria by using an image analysis system. There were significantly higher densities of MMP-8 and MMP-9 positive cells in the lamina propria of bronchiectatic than control airways. In bronchiectatic airways, the densities of MMP-8 and MMP-9 positive cells correlated with each other and with neutrophil density, but not with macrophage density. In control airways, a significant correlation was found between MMP-8 with neutrophil and MMP-9 with macrophage densities. An overexpression of neutrophil matrix metalloproteinases in bronchiectatic airways could help explain the continuation of airway destruction in bronchiectasis. In view of the clinical availability of matrix metalloproteinase antagonists, the results presented here could have a significant impact on the development of novel therapies of this untreatable disease.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong