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Article: Macrophages, neutrophils and tumour necrosis factor-α expression in bronchiectatic airways in vivo
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TitleMacrophages, neutrophils and tumour necrosis factor-α expression in bronchiectatic airways in vivo
 
AuthorsZheng, L1
Shum, IH1
Tipoe, GL1
Leung, R1
Lam, WK1
Ooi, GC1
Tsang, KW1
 
Issue Date2001
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
 
CitationRespiratory Medicine, 2001, v. 95 n. 10, p. 792-798 [How to Cite?]
DOI: http://dx.doi.org/10.1053/rmed.2001.1155
 
AbstractBronchiectasis is increasingly being recognized as an inflammatory condition of the airways in which pathological permanent dilation occurs. We have obtained endobronchial biopsies in 14 patients with stable bronchiectasis and 15 control subjects. Airway neutrophils, macrophages and tumour necrosis factor-α (TNFα)-positive cells were stained with monoclonal antibodies and the densities of positive cells in the lamina propria were determined by using a computer image analyser. There was significantly higher neutrophil, macrophage and TNFα-positive cell densities in the lamina propria of bronchiectatic than control airways (P<0·001, P<0·001 and P=0·0002, respectively). Airway neutrophil density, in bronchiectasis but not in controls, correlated with TNFα-positive cell density (r=0·71, P=0·004). A significant correlation between airway macrophage and TNFα-positive cell densities was demonstrated in both control and bronchiectatic airways (r=0·63, P=0·016 and r=0·60, P=0·02 respectively). Neutrophil density negatively correlated with per cent forced vital capacity (FVC%) predicted among patients with bronchiectasis (r=-0·53, P=0·04). Bronchiectasis patients who were regular sputum producers had a significantly higher macrophage, but not neutrophil density, than their counterparts (P=0·02 and P=0·48 respectively). Our original findings suggest that airway macrophages could contribute to neutrophil influx into airway walls through their production of TNFα and therefore play an important role in the pathogenesis of bronchiectasis. © 2001 Harcourt Publishers Ltd.
 
ISSN0954-6111
2013 Impact Factor: 2.917
 
DOIhttp://dx.doi.org/10.1053/rmed.2001.1155
 
ISI Accession Number IDWOS:000171557600004
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZheng, L
 
dc.contributor.authorShum, IH
 
dc.contributor.authorTipoe, GL
 
dc.contributor.authorLeung, R
 
dc.contributor.authorLam, WK
 
dc.contributor.authorOoi, GC
 
dc.contributor.authorTsang, KW
 
dc.date.accessioned2012-06-26T05:55:45Z
 
dc.date.available2012-06-26T05:55:45Z
 
dc.date.issued2001
 
dc.description.abstractBronchiectasis is increasingly being recognized as an inflammatory condition of the airways in which pathological permanent dilation occurs. We have obtained endobronchial biopsies in 14 patients with stable bronchiectasis and 15 control subjects. Airway neutrophils, macrophages and tumour necrosis factor-α (TNFα)-positive cells were stained with monoclonal antibodies and the densities of positive cells in the lamina propria were determined by using a computer image analyser. There was significantly higher neutrophil, macrophage and TNFα-positive cell densities in the lamina propria of bronchiectatic than control airways (P<0·001, P<0·001 and P=0·0002, respectively). Airway neutrophil density, in bronchiectasis but not in controls, correlated with TNFα-positive cell density (r=0·71, P=0·004). A significant correlation between airway macrophage and TNFα-positive cell densities was demonstrated in both control and bronchiectatic airways (r=0·63, P=0·016 and r=0·60, P=0·02 respectively). Neutrophil density negatively correlated with per cent forced vital capacity (FVC%) predicted among patients with bronchiectasis (r=-0·53, P=0·04). Bronchiectasis patients who were regular sputum producers had a significantly higher macrophage, but not neutrophil density, than their counterparts (P=0·02 and P=0·48 respectively). Our original findings suggest that airway macrophages could contribute to neutrophil influx into airway walls through their production of TNFα and therefore play an important role in the pathogenesis of bronchiectasis. © 2001 Harcourt Publishers Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationRespiratory Medicine, 2001, v. 95 n. 10, p. 792-798 [How to Cite?]
DOI: http://dx.doi.org/10.1053/rmed.2001.1155
 
dc.identifier.doihttp://dx.doi.org/10.1053/rmed.2001.1155
 
dc.identifier.epage798
 
dc.identifier.hkuros68134
 
dc.identifier.hkuros64250
 
dc.identifier.isiWOS:000171557600004
 
dc.identifier.issn0954-6111
2013 Impact Factor: 2.917
 
dc.identifier.issue10
 
dc.identifier.pmid11601743
 
dc.identifier.scopuseid_2-s2.0-0034764130
 
dc.identifier.spage792
 
dc.identifier.urihttp://hdl.handle.net/10722/149598
 
dc.identifier.volume95
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/rmed
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofRespiratory Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAged
 
dc.subject.meshAntigens, Cd - Analysis
 
dc.subject.meshAntigens, Differentiation, Myelomonocytic - Analysis
 
dc.subject.meshBiological Markers - Analysis
 
dc.subject.meshBronchiectasis - Immunology - Pathology
 
dc.subject.meshBronchoscopy
 
dc.subject.meshCase-Control Studies
 
dc.subject.meshCell Count
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshLung - Immunology - Pathology
 
dc.subject.meshMacrophages - Metabolism - Pathology
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNeutrophils - Metabolism - Pathology
 
dc.subject.meshPancreatic Elastase - Analysis
 
dc.subject.meshStatistics, Nonparametric
 
dc.subject.meshTumor Necrosis Factor-Alpha - Metabolism
 
dc.titleMacrophages, neutrophils and tumour necrosis factor-α expression in bronchiectatic airways in vivo
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong