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Article: LMP1 of Epstein-Barr virus induces proliferation of primary mouse embryonic fibroblasts and cooperatively transforms the cells with a p16- insensitive CDK4 oncogene

TitleLMP1 of Epstein-Barr virus induces proliferation of primary mouse embryonic fibroblasts and cooperatively transforms the cells with a p16- insensitive CDK4 oncogene
Authors
Issue Date2000
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2000, v. 74 n. 2, p. 883-891 How to Cite?
AbstractThe latent membrane protein LMP1 of Epstein-Barr virus (EBV) is often present in EBV-associated malignancies including nasopharyngeal carcinoma and Hodgkin's lymphoma. Previous work demonstrates that the LMP1 gene of EBV is sufficient to transform certain established rodent fibroblast cell lines and to induce the tumorigenicity of some human epithelial cell lines. In addition, LMP1 plays pleiotropic roles in cell growth arrest, differentiation, and apoptosis, depending on the background of the target cells. To examine the roles of LMP1 in cell proliferation and growth regulation in primary culture cells, we constructed a recombinant retrovirus containing an LMP1 gene. With this retrovirus, LMP1 was shown to stimulate the proliferation of primary mouse embryonic fibroblasts (MEF cells). It has a mitogenic activity for MEF cells, as demonstrated by an immediate induction of cell doubling time. In addition, it significantly extends the passage number of MEF cells to more than 30 after retroviral infection, compared with less than 5 for uninfected MEF cells. Furthermore, LMP1 cooperates with a p16-insensitive CDK4(R24C) oncogene in transforming MEF cells. Our results provide the first evidence of the abilities of the LMP1 gene, acting alone, to effectively induce the proliferation of primary MEF cells and of its cooperativity with another cellular oncogene in transforming primary cells.
Persistent Identifierhttp://hdl.handle.net/10722/149588
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Xen_US
dc.contributor.authorSham, JSTen_US
dc.contributor.authorNg, MHen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorZhang, Den_US
dc.contributor.authorLowe, SWen_US
dc.contributor.authorCao, Len_US
dc.date.accessioned2012-06-26T05:55:38Z-
dc.date.available2012-06-26T05:55:38Z-
dc.date.issued2000en_US
dc.identifier.citationJournal Of Virology, 2000, v. 74 n. 2, p. 883-891en_US
dc.identifier.issn0022-538Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/149588-
dc.description.abstractThe latent membrane protein LMP1 of Epstein-Barr virus (EBV) is often present in EBV-associated malignancies including nasopharyngeal carcinoma and Hodgkin's lymphoma. Previous work demonstrates that the LMP1 gene of EBV is sufficient to transform certain established rodent fibroblast cell lines and to induce the tumorigenicity of some human epithelial cell lines. In addition, LMP1 plays pleiotropic roles in cell growth arrest, differentiation, and apoptosis, depending on the background of the target cells. To examine the roles of LMP1 in cell proliferation and growth regulation in primary culture cells, we constructed a recombinant retrovirus containing an LMP1 gene. With this retrovirus, LMP1 was shown to stimulate the proliferation of primary mouse embryonic fibroblasts (MEF cells). It has a mitogenic activity for MEF cells, as demonstrated by an immediate induction of cell doubling time. In addition, it significantly extends the passage number of MEF cells to more than 30 after retroviral infection, compared with less than 5 for uninfected MEF cells. Furthermore, LMP1 cooperates with a p16-insensitive CDK4(R24C) oncogene in transforming MEF cells. Our results provide the first evidence of the abilities of the LMP1 gene, acting alone, to effectively induce the proliferation of primary MEF cells and of its cooperativity with another cellular oncogene in transforming primary cells.en_US
dc.languageengen_US
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_US
dc.relation.ispartofJournal of Virologyen_US
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.subject.meshAnimalsen_US
dc.subject.meshCarrier Proteins - Pharmacologyen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Transformation, Neoplasticen_US
dc.subject.meshCell Transformation, Viralen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCyclin-Dependent Kinase 4en_US
dc.subject.meshCyclin-Dependent Kinase Inhibitor P16en_US
dc.subject.meshCyclin-Dependent Kinases - Geneticsen_US
dc.subject.meshFibroblasts - Cytologyen_US
dc.subject.meshGenetic Vectors - Geneticsen_US
dc.subject.meshHela Cellsen_US
dc.subject.meshHerpesvirus 4, Human - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshOncogene Proteins, Viral - Biosynthesis - Genetics - Physiologyen_US
dc.subject.meshProto-Oncogene Proteinsen_US
dc.subject.meshRetroviridae - Geneticsen_US
dc.subject.meshTelomerase - Metabolismen_US
dc.subject.meshViral Matrix Proteins - Biosynthesis - Genetics - Physiologyen_US
dc.titleLMP1 of Epstein-Barr virus induces proliferation of primary mouse embryonic fibroblasts and cooperatively transforms the cells with a p16- insensitive CDK4 oncogeneen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/JVI.74.2.883-891.2000en_US
dc.identifier.pmid10623751-
dc.identifier.scopuseid_2-s2.0-0033986419en_US
dc.identifier.hkuros47795-
dc.identifier.hkuros53532-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033986419&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume74en_US
dc.identifier.issue2en_US
dc.identifier.spage883en_US
dc.identifier.epage891en_US
dc.identifier.isiWOS:000084421700032-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYang, X=18839356200en_US
dc.identifier.scopusauthoridSham, JST=7101655565en_US
dc.identifier.scopusauthoridNg, MH=7202076421en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridZhang, D=7405361705en_US
dc.identifier.scopusauthoridLowe, SW=15064198100en_US
dc.identifier.scopusauthoridCao, L=7401637818en_US

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