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Article: Technical note: A new model for quantitative analysis of brain oedema resolution into the ventricles and the subarachnoid space

TitleTechnical note: A new model for quantitative analysis of brain oedema resolution into the ventricles and the subarachnoid space
Authors
Issue Date1999
PublisherSpringer-Verlag Wien. The Journal's web site is located at http://www.springer.at/acta_neuro
Citation
Acta Neurochirurgica, 1999, v. 141 n. 1, p. 89-92 How to Cite?
AbstractObjective. The aim of the current study was to develop an experimental animal model for quantitative analysis of oedema resolution via the subarachnoid space and the ventricular system using fluorescent oedema markers. Methods. Artificial cerebrospinal fluid (CSF) containing TRITC-albumin (MW 67.000D) and Na+-fluorescein (MW 376D) was continuously infused into the white matter of the left frontal lobe of New Zealand white rabbits (n = 6) at a rate of 100 μl/h for 3 hrs. A closed cranial window for superfusion of the brain surface with artificial CSF fluid (3 ml/h) was implanted above the left parietal cortex for measurement of the fluorescence markers in the subarachnoid space. Uptake of the fluorescence indicators into the ventricles was quantified by ventriculo-cisternal perfusion (3 ml/h). The effluates were collected at 30 min intervals for 3 hrs after the start of infusion. Clearance of the oedema fluid into the perfusates was measured by fluorescence spectrophotometry. Results. At an intracranial pressure of 15.0 ± 1.7 mmHg (mean ± SEM) both indicators started to accumulate in the subarachnoid and ventricular perfusates at 90 min following onset of oedema fluid infusion. The concentrations of the indicators in the ventricular system increased to 7.7 ± 5.1% of Na+-fluorescein and 16.1 ± 13.0% of TRITC-albumin of the total amount infused were recovered in the ventricular system at 3 hours after start of the oedema infusion, while 3.4 ± 3.2% of Na+-fluorescein and 3.7% ± 3.2 of TRITC-albumin, respectively, were found in the effluates of the subarachnoid space. Conclusion. The present study demonstrates that resolution of vasogenic brain oedema into the cerebral ventricular system and the subarachnoid space following its entry into cerbral white matter can be quantitatively analysed using fluorescence markers, which serve as oedema fluid indicators. The results indicate that the oedema fluid is cleared not only into the ventricular system but also via the subarachnoid space.
Persistent Identifierhttp://hdl.handle.net/10722/149577
ISSN
2015 Impact Factor: 1.617
2015 SCImago Journal Rankings: 0.833
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorUhl, Een_US
dc.contributor.authorWrba, Een_US
dc.contributor.authorNehring, Ven_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorBaethmann, Aen_US
dc.contributor.authorReulen, HJen_US
dc.date.accessioned2012-06-26T05:55:29Z-
dc.date.available2012-06-26T05:55:29Z-
dc.date.issued1999en_US
dc.identifier.citationActa Neurochirurgica, 1999, v. 141 n. 1, p. 89-92en_US
dc.identifier.issn0001-6268en_US
dc.identifier.urihttp://hdl.handle.net/10722/149577-
dc.description.abstractObjective. The aim of the current study was to develop an experimental animal model for quantitative analysis of oedema resolution via the subarachnoid space and the ventricular system using fluorescent oedema markers. Methods. Artificial cerebrospinal fluid (CSF) containing TRITC-albumin (MW 67.000D) and Na+-fluorescein (MW 376D) was continuously infused into the white matter of the left frontal lobe of New Zealand white rabbits (n = 6) at a rate of 100 μl/h for 3 hrs. A closed cranial window for superfusion of the brain surface with artificial CSF fluid (3 ml/h) was implanted above the left parietal cortex for measurement of the fluorescence markers in the subarachnoid space. Uptake of the fluorescence indicators into the ventricles was quantified by ventriculo-cisternal perfusion (3 ml/h). The effluates were collected at 30 min intervals for 3 hrs after the start of infusion. Clearance of the oedema fluid into the perfusates was measured by fluorescence spectrophotometry. Results. At an intracranial pressure of 15.0 ± 1.7 mmHg (mean ± SEM) both indicators started to accumulate in the subarachnoid and ventricular perfusates at 90 min following onset of oedema fluid infusion. The concentrations of the indicators in the ventricular system increased to 7.7 ± 5.1% of Na+-fluorescein and 16.1 ± 13.0% of TRITC-albumin of the total amount infused were recovered in the ventricular system at 3 hours after start of the oedema infusion, while 3.4 ± 3.2% of Na+-fluorescein and 3.7% ± 3.2 of TRITC-albumin, respectively, were found in the effluates of the subarachnoid space. Conclusion. The present study demonstrates that resolution of vasogenic brain oedema into the cerebral ventricular system and the subarachnoid space following its entry into cerbral white matter can be quantitatively analysed using fluorescence markers, which serve as oedema fluid indicators. The results indicate that the oedema fluid is cleared not only into the ventricular system but also via the subarachnoid space.en_US
dc.languageengen_US
dc.publisherSpringer-Verlag Wien. The Journal's web site is located at http://www.springer.at/acta_neuroen_US
dc.relation.ispartofActa Neurochirurgicaen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain Edema - Etiology - Metabolismen_US
dc.subject.meshCerebral Ventricles - Secretionen_US
dc.subject.meshCerebrospinal Fluid - Secretionen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshExtracellular Space - Metabolismen_US
dc.subject.meshFluorescent Dyes - Pharmacokineticsen_US
dc.subject.meshIntracranial Pressureen_US
dc.subject.meshMaleen_US
dc.subject.meshRabbitsen_US
dc.subject.meshRemission, Spontaneousen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.subject.meshSubarachnoid Space - Secretionen_US
dc.titleTechnical note: A new model for quantitative analysis of brain oedema resolution into the ventricles and the subarachnoid spaceen_US
dc.typeArticleen_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s007010050270en_US
dc.identifier.pmid10071691-
dc.identifier.scopuseid_2-s2.0-0032922559en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032922559&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume141en_US
dc.identifier.issue1en_US
dc.identifier.spage89en_US
dc.identifier.epage92en_US
dc.identifier.isiWOS:000078554900028-
dc.publisher.placeAustriaen_US
dc.identifier.scopusauthoridUhl, E=7004972666en_US
dc.identifier.scopusauthoridWrba, E=6507130530en_US
dc.identifier.scopusauthoridNehring, V=6602301916en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US
dc.identifier.scopusauthoridBaethmann, A=7004994793en_US
dc.identifier.scopusauthoridReulen, HJ=7006146584en_US

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