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Article: Relevance of Calcium Homeostasis in Glial Cell Swelling from Acidosis

TitleRelevance of Calcium Homeostasis in Glial Cell Swelling from Acidosis
Authors
Issue Date1998
Citation
Acta Neurochirurgica, Supplement, 1998, v. 1998 SUPPL. 71, p. 203-205 How to Cite?
AbstractTissue acidosis from trauma or ischemia induces cytotoxic brain edema, mainly affecting astrocytes. In vitro, lactacidosis induces a dose-dependent swelling of glial cells. Activation of membrane transporters and channels, also involved in regulation of intracellular pH (pHi), has been identified as underlying mechanism, although details are poorly understood. We have currently studied whether Ca2+-ions play a role in acidosis-induced glial swelling and the associated intracellular acidification. The medium pH of a cell suspension (C6 glioma) was lowered from control (7.4) to 6.2 by lactic acid. Cell volume (CV) and pHi were assessed by flow cytometry. During acidosis in normal medium (2.2mM Ca2+) CV reached a maximum of 125.1%. In a calcium-free medium swelling from acidosis was inhibited by 74%, while additional buffering of intracellular calcium (Ca2+) by BAPTA-AM had no further effect. Buffering of Ca2+ i alone did not affect the CV increase from acidosis at all. pHi which is decreasing during acidosis was not influenced by the above modifications. The present experiments indicate that lactacidosis-induced glial swelling depends on the presence of extracellular Ca2+-ions, while alterations of Ca2+ i do not seem to be involved.
Persistent Identifierhttp://hdl.handle.net/10722/149573
ISSN
2015 SCImago Journal Rankings: 0.379
References

 

DC FieldValueLanguage
dc.contributor.authorPlesnila, Nen_US
dc.contributor.authorRingel, Fen_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorStaub, Fen_US
dc.contributor.authorBaethmann, Aen_US
dc.date.accessioned2012-06-26T05:55:26Z-
dc.date.available2012-06-26T05:55:26Z-
dc.date.issued1998en_US
dc.identifier.citationActa Neurochirurgica, Supplement, 1998, v. 1998 SUPPL. 71, p. 203-205en_US
dc.identifier.issn0065-1419en_US
dc.identifier.urihttp://hdl.handle.net/10722/149573-
dc.description.abstractTissue acidosis from trauma or ischemia induces cytotoxic brain edema, mainly affecting astrocytes. In vitro, lactacidosis induces a dose-dependent swelling of glial cells. Activation of membrane transporters and channels, also involved in regulation of intracellular pH (pHi), has been identified as underlying mechanism, although details are poorly understood. We have currently studied whether Ca2+-ions play a role in acidosis-induced glial swelling and the associated intracellular acidification. The medium pH of a cell suspension (C6 glioma) was lowered from control (7.4) to 6.2 by lactic acid. Cell volume (CV) and pHi were assessed by flow cytometry. During acidosis in normal medium (2.2mM Ca2+) CV reached a maximum of 125.1%. In a calcium-free medium swelling from acidosis was inhibited by 74%, while additional buffering of intracellular calcium (Ca2+) by BAPTA-AM had no further effect. Buffering of Ca2+ i alone did not affect the CV increase from acidosis at all. pHi which is decreasing during acidosis was not influenced by the above modifications. The present experiments indicate that lactacidosis-induced glial swelling depends on the presence of extracellular Ca2+-ions, while alterations of Ca2+ i do not seem to be involved.en_US
dc.languageengen_US
dc.relation.ispartofActa Neurochirurgica, Supplementen_US
dc.subject.meshAcid-Base Equilibrium - Physiologyen_US
dc.subject.meshAcidosis, Lactic - Physiopathologyen_US
dc.subject.meshBrain Neoplasmsen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCalcium Channels - Physiologyen_US
dc.subject.meshCell Death - Physiologyen_US
dc.subject.meshCell Size - Physiologyen_US
dc.subject.meshGliomaen_US
dc.subject.meshHomeostasis - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshNeuroglia - Physiologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleRelevance of Calcium Homeostasis in Glial Cell Swelling from Acidosisen_US
dc.typeArticleen_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9779184-
dc.identifier.scopuseid_2-s2.0-0032238806en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032238806&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1998en_US
dc.identifier.issueSUPPL. 71en_US
dc.identifier.spage203en_US
dc.identifier.epage205en_US
dc.publisher.placeAustriaen_US
dc.identifier.scopusauthoridPlesnila, N=7003609441en_US
dc.identifier.scopusauthoridRingel, F=6602136569en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US
dc.identifier.scopusauthoridStaub, F=7006611117en_US
dc.identifier.scopusauthoridBaethmann, A=7004994793en_US

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