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Article: Expression profile of senescence-associated ß-galactosidase and activation of telomerase in human ovarian surface epithelial cells undergoing immortalization

TitleExpression profile of senescence-associated ß-galactosidase and activation of telomerase in human ovarian surface epithelial cells undergoing immortalization
Authors
Issue Date1998
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 1998, v. 13 n. 5, p. 951-956 How to Cite?
AbstractSenescence is a specific physiological stage of cells characterized by long population doubling time. It accounts for the inability of normal somatic cells to undergo indefinite cell division. As the number of population doublings increase, cell cycle regulatory mechanisms come into play and signal cells to exit the cell cycle and become senescent. Senescence has been implicated in the aging process and may function as a tumor suppressor mechanism in human cells. The ability to measure the degree of cellular senescence is important in understanding the biological processes regulating cell aging and immortalization. Senescent cells exhibit an enzyme termed senescence-associated ß-galactosidase (SA-ß-Gal) which is detected at pH 6.0 via histochemical staining. Cells immortalized by viral oncogenes often enter a stage of crisis at the early phase of immortalization. The cells at crisis have a long population doubling time. Cells at the crisis stage resemble senescent cells and the expression of SA-ß-Gal may be used to monitor the process of immortalization. In this study the expression profile of SA-ß-Gal was examined in human ovarian surface epithelial cells (HOSE 6-3) undergoing immortalization by the human papilloma viral oncogene E6 and E7 (HPV E6 and E7). Our results showed a low percentage (12.0%) of HOSE 6-3 cells expressing SA-ß-Gal activity at the pre-crisis stage. The percentage of HOSE 6-3 cells expressing SA-ß-Gal activity was highest (39.2%) at the crisis stage. When HOSE 6-3 cells achieved immortalized status there was a sharp decrease in cells (1.3%) expressing SA-ß-Gal activity. In addition, an inverse relationship between the expression of SA-ß-Gal activity and telomerase activity was noted in cells undergoing immortalization. The results confirm that the SA-ß-Gal enzyme is a good marker for monitoring the population of cells undergoing senescence at different stages of immortalization and that telomerase activation is a characteristic feature of post-crisis cells.
Persistent Identifierhttp://hdl.handle.net/10722/149571
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLitaker, JRen_US
dc.contributor.authorPan, Jen_US
dc.contributor.authorCheung, YCen_US
dc.contributor.authorZhang, DKen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorWong, SCHen_US
dc.contributor.authorWan, TSKen_US
dc.contributor.authorTsao, SWen_US
dc.date.accessioned2012-06-26T05:55:25Z-
dc.date.available2012-06-26T05:55:25Z-
dc.date.issued1998en_US
dc.identifier.citationInternational Journal Of Oncology, 1998, v. 13 n. 5, p. 951-956en_US
dc.identifier.issn1019-6439en_US
dc.identifier.urihttp://hdl.handle.net/10722/149571-
dc.description.abstractSenescence is a specific physiological stage of cells characterized by long population doubling time. It accounts for the inability of normal somatic cells to undergo indefinite cell division. As the number of population doublings increase, cell cycle regulatory mechanisms come into play and signal cells to exit the cell cycle and become senescent. Senescence has been implicated in the aging process and may function as a tumor suppressor mechanism in human cells. The ability to measure the degree of cellular senescence is important in understanding the biological processes regulating cell aging and immortalization. Senescent cells exhibit an enzyme termed senescence-associated ß-galactosidase (SA-ß-Gal) which is detected at pH 6.0 via histochemical staining. Cells immortalized by viral oncogenes often enter a stage of crisis at the early phase of immortalization. The cells at crisis have a long population doubling time. Cells at the crisis stage resemble senescent cells and the expression of SA-ß-Gal may be used to monitor the process of immortalization. In this study the expression profile of SA-ß-Gal was examined in human ovarian surface epithelial cells (HOSE 6-3) undergoing immortalization by the human papilloma viral oncogene E6 and E7 (HPV E6 and E7). Our results showed a low percentage (12.0%) of HOSE 6-3 cells expressing SA-ß-Gal activity at the pre-crisis stage. The percentage of HOSE 6-3 cells expressing SA-ß-Gal activity was highest (39.2%) at the crisis stage. When HOSE 6-3 cells achieved immortalized status there was a sharp decrease in cells (1.3%) expressing SA-ß-Gal activity. In addition, an inverse relationship between the expression of SA-ß-Gal activity and telomerase activity was noted in cells undergoing immortalization. The results confirm that the SA-ß-Gal enzyme is a good marker for monitoring the population of cells undergoing senescence at different stages of immortalization and that telomerase activation is a characteristic feature of post-crisis cells.en_US
dc.languageengen_US
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_US
dc.relation.ispartofInternational Journal of Oncologyen_US
dc.subject.meshBiological Markersen_US
dc.subject.meshCell Agingen_US
dc.subject.meshCell Differentiation - Physiologyen_US
dc.subject.meshCell Transformation, Neoplasticen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshEpithelial Cells - Cytology - Enzymologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshOvary - Cytology - Enzymologyen_US
dc.subject.meshTelomerase - Metabolismen_US
dc.subject.meshBeta-Galactosidase - Biosynthesisen_US
dc.titleExpression profile of senescence-associated ß-galactosidase and activation of telomerase in human ovarian surface epithelial cells undergoing immortalizationen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9772284-
dc.identifier.scopuseid_2-s2.0-0031732328en_US
dc.identifier.hkuros39084-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031732328&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue5en_US
dc.identifier.spage951en_US
dc.identifier.epage956en_US
dc.identifier.isiWOS:000076806800008-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridLitaker, JR=6603079079en_US
dc.identifier.scopusauthoridPan, J=7404098399en_US
dc.identifier.scopusauthoridCheung, YC=7202111087en_US
dc.identifier.scopusauthoridZhang, DK=7405361705en_US
dc.identifier.scopusauthoridLiu, Y=26643293600en_US
dc.identifier.scopusauthoridWong, SCH=7404590793en_US
dc.identifier.scopusauthoridWan, TSK=25623981600en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US

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