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Article: Effect of Mild and Moderate Hypothermia on the Acidosis-Induced Swelling of Glial Cells

TitleEffect of Mild and Moderate Hypothermia on the Acidosis-Induced Swelling of Glial Cells
Authors
Issue Date1997
Citation
Acta Neurochirurgica, Supplement, 1997, v. 1997 n. 70, p. 262-264 How to Cite?
AbstractThe effect of mild (32°C) and moderate (27°C) hypothermia was analyzed on the cell volume and intracellular pH (pHi) of C6 glioma cells at normal pH and during lactacidosis at pH 6.2 in vitro. The cells were suspended in an incubation chamber under continuous control of pH, PO2 and temperature. Cell swelling was quantified by an advanced Coulter-system. pHi was measured by flow cytometry using the fluorescent dye bis-carboxyethyl carboxyfluorescein (BCECF). Following a control period at 37°C, the ambient temperature was decreased to 32°C for 30 min, and subsequently to 27°C for another 30 min. Hypothermia alone led to an immediate and significant cell volume increase of 107.3 ± 0.4% (mean ± SEM) of control after 30 min at 32°C, and further swelling to 110.5 ± 0.9% after 30 min at 27°C. Yet, hypothermia (27°C) afforded partial protection against the acidosis-induced cell swelling at pH 6.2, which was reaching to 120.4 ± 0.9% in the normothermic control group after 60 min, while only to 111.3 ± 0.9% at 27°C. Hypothermia, however, was associated with a more pronounced decrease of the pHi during acidosis (6.3 ± 0.04) as compared to that of the normothermic control falling then to 6.5 ± 0.03. The results demonstrate that mild and moderate hypothermia induce glial cell swelling, but simultaneously inhibit cell swelling from acidosis. The protection against cell swelling, however, has its price as indicated by the enhancement of the intracellular acidification.
Persistent Identifierhttp://hdl.handle.net/10722/149568
ISSN
2015 SCImago Journal Rankings: 0.379
References

 

DC FieldValueLanguage
dc.contributor.authorPlesnila, Nen_US
dc.contributor.authorRingel, Fen_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorPeters, Jen_US
dc.contributor.authorStaub, Fen_US
dc.contributor.authorBaethmann, Aen_US
dc.date.accessioned2012-06-26T05:55:23Z-
dc.date.available2012-06-26T05:55:23Z-
dc.date.issued1997en_US
dc.identifier.citationActa Neurochirurgica, Supplement, 1997, v. 1997 n. 70, p. 262-264en_US
dc.identifier.issn0065-1419en_US
dc.identifier.urihttp://hdl.handle.net/10722/149568-
dc.description.abstractThe effect of mild (32°C) and moderate (27°C) hypothermia was analyzed on the cell volume and intracellular pH (pHi) of C6 glioma cells at normal pH and during lactacidosis at pH 6.2 in vitro. The cells were suspended in an incubation chamber under continuous control of pH, PO2 and temperature. Cell swelling was quantified by an advanced Coulter-system. pHi was measured by flow cytometry using the fluorescent dye bis-carboxyethyl carboxyfluorescein (BCECF). Following a control period at 37°C, the ambient temperature was decreased to 32°C for 30 min, and subsequently to 27°C for another 30 min. Hypothermia alone led to an immediate and significant cell volume increase of 107.3 ± 0.4% (mean ± SEM) of control after 30 min at 32°C, and further swelling to 110.5 ± 0.9% after 30 min at 27°C. Yet, hypothermia (27°C) afforded partial protection against the acidosis-induced cell swelling at pH 6.2, which was reaching to 120.4 ± 0.9% in the normothermic control group after 60 min, while only to 111.3 ± 0.9% at 27°C. Hypothermia, however, was associated with a more pronounced decrease of the pHi during acidosis (6.3 ± 0.04) as compared to that of the normothermic control falling then to 6.5 ± 0.03. The results demonstrate that mild and moderate hypothermia induce glial cell swelling, but simultaneously inhibit cell swelling from acidosis. The protection against cell swelling, however, has its price as indicated by the enhancement of the intracellular acidification.en_US
dc.languageengen_US
dc.relation.ispartofActa Neurochirurgica, Supplementen_US
dc.subject.meshAcidosis, Lactic - Pathologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Size - Physiologyen_US
dc.subject.meshHypothermia, Induceden_US
dc.subject.meshNeuroglia - Pathologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleEffect of Mild and Moderate Hypothermia on the Acidosis-Induced Swelling of Glial Cellsen_US
dc.typeArticleen_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9416341en_US
dc.identifier.scopuseid_2-s2.0-0031308781en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031308781&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume1997en_US
dc.identifier.issue70en_US
dc.identifier.spage262en_US
dc.identifier.epage264en_US
dc.publisher.placeAustriaen_US
dc.identifier.scopusauthoridPlesnila, N=7003609441en_US
dc.identifier.scopusauthoridRingel, F=6602136569en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US
dc.identifier.scopusauthoridPeters, J=7404191248en_US
dc.identifier.scopusauthoridStaub, F=7006611117en_US
dc.identifier.scopusauthoridBaethmann, A=7004994793en_US

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