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Article: The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in human benign and malignant epithelial lesions of the parotid gland

TitleThe assessment of proliferating cell nuclear antigen (PCNA) immunostaining in human benign and malignant epithelial lesions of the parotid gland
Authors
Issue Date1997
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/oraloncology
Citation
European Journal Of Cancer Part B: Oral Oncology, 1997, v. 33 n. 1, p. 29-35 How to Cite?
AbstractImmunoreactivity of proliferating cell nuclear antigen (PCNA) was assessed in formalin-fixed, paraffin-embedded sections from human normal parotid gland (N; n = 12), chronic sialadenitis (CS; n = 8), Warthin's tumour (W; n = 10), benign pleomorphic adenoma (BPA; n = 11), mucoepidermoid carcinoma (MEC; n = 14), carcinoma in pleomorphic adenoma (CPA; n = 10) and adenoid cystic carcinoma (ACC; n = n) of the parotid gland, using the monoclonal antibody PC 10. The morphometric parameters measured comprised PCNA labelling indices (PI = the numerical percentage of PCNA positive nuclei) and volume densities of PCNA positive nuclei (V(v), PEP = the relative volume of positive nuclei per unit volume of reference epithelium). All parameters were expressed in relation to total positive, as well as to strongly- and weakly-positive nuclei. In general, the values of PCNA parameters increased progressively in benign lesions in comparison with the N group, and in malignant neoplasms in comparison with non-neoplastic groups and benign lesions. The strongly-positive parameters showed more statistically significant differences than weakly-positive ones, suggesting that weakly-stained nuclei may include some non-cycling cells and, therefore, that weakly-positive parameters may not be reliable proliferation markers. Values for all parameters in CPA were significantly higher than those in BPA, suggesting that these parameters may be used as diagnostic discriminators. Spearman rank correlation analysis showed a highly positive correlation between the morphometric parameters and the severity of the lesions. Furthermore, the mean values of PI(SP) were significantly higher in patients who died of the malignant tumours than in those patients who survived. Our results indicate that PCNA indices might be useful markers for discriminating between benign (BPA) and malignant tumours of the parotid gland and that the parameter PI(SP) may have prognostic applications.
Persistent Identifierhttp://hdl.handle.net/10722/149563
ISSN
2002 Impact Factor: 1.873
2000 SCImago Journal Rankings: 0.448
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Qen_US
dc.contributor.authorWhite, FHen_US
dc.contributor.authorTipoe, GLen_US
dc.date.accessioned2012-06-26T05:55:18Z-
dc.date.available2012-06-26T05:55:18Z-
dc.date.issued1997en_US
dc.identifier.citationEuropean Journal Of Cancer Part B: Oral Oncology, 1997, v. 33 n. 1, p. 29-35en_US
dc.identifier.issn0964-1955en_US
dc.identifier.urihttp://hdl.handle.net/10722/149563-
dc.description.abstractImmunoreactivity of proliferating cell nuclear antigen (PCNA) was assessed in formalin-fixed, paraffin-embedded sections from human normal parotid gland (N; n = 12), chronic sialadenitis (CS; n = 8), Warthin's tumour (W; n = 10), benign pleomorphic adenoma (BPA; n = 11), mucoepidermoid carcinoma (MEC; n = 14), carcinoma in pleomorphic adenoma (CPA; n = 10) and adenoid cystic carcinoma (ACC; n = n) of the parotid gland, using the monoclonal antibody PC 10. The morphometric parameters measured comprised PCNA labelling indices (PI = the numerical percentage of PCNA positive nuclei) and volume densities of PCNA positive nuclei (V(v), PEP = the relative volume of positive nuclei per unit volume of reference epithelium). All parameters were expressed in relation to total positive, as well as to strongly- and weakly-positive nuclei. In general, the values of PCNA parameters increased progressively in benign lesions in comparison with the N group, and in malignant neoplasms in comparison with non-neoplastic groups and benign lesions. The strongly-positive parameters showed more statistically significant differences than weakly-positive ones, suggesting that weakly-stained nuclei may include some non-cycling cells and, therefore, that weakly-positive parameters may not be reliable proliferation markers. Values for all parameters in CPA were significantly higher than those in BPA, suggesting that these parameters may be used as diagnostic discriminators. Spearman rank correlation analysis showed a highly positive correlation between the morphometric parameters and the severity of the lesions. Furthermore, the mean values of PI(SP) were significantly higher in patients who died of the malignant tumours than in those patients who survived. Our results indicate that PCNA indices might be useful markers for discriminating between benign (BPA) and malignant tumours of the parotid gland and that the parameter PI(SP) may have prognostic applications.en_US
dc.languageengen_US
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/oraloncologyen_US
dc.relation.ispartofEuropean Journal of Cancer Part B: Oral Oncologyen_US
dc.subject.meshAdenocarcinoma - Metabolismen_US
dc.subject.meshAdenolymphoma - Metabolismen_US
dc.subject.meshAdenoma, Pleomorphic - Metabolismen_US
dc.subject.meshCarcinoma, Adenoid Cystic - Metabolismen_US
dc.subject.meshCarcinoma, Mucoepidermoid - Metabolismen_US
dc.subject.meshFollow-Up Studiesen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshParotid Diseases - Metabolism - Pathologyen_US
dc.subject.meshParotid Gland - Metabolismen_US
dc.subject.meshParotid Neoplasms - Metabolism - Pathologyen_US
dc.subject.meshProliferating Cell Nuclear Antigen - Metabolismen_US
dc.subject.meshSialadenitis - Metabolismen_US
dc.subject.meshStatistics, Nonparametricen_US
dc.subject.meshSurvival Analysisen_US
dc.subject.meshTumor Markers, Biological - Metabolismen_US
dc.titleThe assessment of proliferating cell nuclear antigen (PCNA) immunostaining in human benign and malignant epithelial lesions of the parotid glanden_US
dc.typeArticleen_US
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_US
dc.identifier.authorityTipoe, GL=rp00371en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0964-1955(96)00038-3en_US
dc.identifier.pmid9192550-
dc.identifier.scopuseid_2-s2.0-0030987114en_US
dc.identifier.hkuros21939-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030987114&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume33en_US
dc.identifier.issue1en_US
dc.identifier.spage29en_US
dc.identifier.epage35en_US
dc.identifier.isiWOS:A1997WR21500007-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridZhu, Q=16026167700en_US
dc.identifier.scopusauthoridWhite, FH=7202578907en_US
dc.identifier.scopusauthoridTipoe, GL=7003550610en_US

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