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Article: Swelling and damage of glial cells by lactacidosis and glutamate: Effect of α-trinositol

TitleSwelling and damage of glial cells by lactacidosis and glutamate: Effect of α-trinositol
Authors
Issue Date1997
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainres
Citation
Brain Research, 1997, v. 766 n. 1-2, p. 285-288 How to Cite?
AbstractThe therapeutical efficacy of α-trinositol (D-myo-inositol-1,2,6- trisphosphate), an isomer of the intracellular messenger IP3, was analyzed on cytotoxic swelling and damage of glial cells in vitro from lactacidosis or glutamate. C6 glioma cells suspended in a physiological medium were either exposed to pH 5.0 by administration of lactic acid, or to 1 mM glutamate. Cell swelling and viability were quantified by flow cytometry. Lactacidosis of pH 5.0 led to an increase in cell volume to 139.7 ± 1.3% within 20 min whereas α-trinositol was reducing the swelling response by approximately 25% (P < 0.01). In addition, at pH 5.0 the fraction of viable cells was lowered from 94.3 ± 0.2% (control) to only 53.8 ± 3.1% after 60 min. α-Trinositol was found to protect also cell viability; at 60 min of lactacidosis 70.2 ± 1.6% of the cells still were viable (P < 0.01). The addition of glutamate (1 mM) to the cell suspension led to a steady increase in cell size, reaching 110% of control at 120 min, irrespectively of whether α-trinositol was added or not.
Persistent Identifierhttp://hdl.handle.net/10722/149562
ISSN
2015 Impact Factor: 2.561
2015 SCImago Journal Rankings: 1.351
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorStaub, Fen_US
dc.contributor.authorPeters, Jen_US
dc.contributor.authorPlesnila, Nen_US
dc.contributor.authorChang, RCCen_US
dc.contributor.authorBaethmann, Aen_US
dc.date.accessioned2012-06-26T05:55:18Z-
dc.date.available2012-06-26T05:55:18Z-
dc.date.issued1997en_US
dc.identifier.citationBrain Research, 1997, v. 766 n. 1-2, p. 285-288en_US
dc.identifier.issn0006-8993en_US
dc.identifier.urihttp://hdl.handle.net/10722/149562-
dc.description.abstractThe therapeutical efficacy of α-trinositol (D-myo-inositol-1,2,6- trisphosphate), an isomer of the intracellular messenger IP3, was analyzed on cytotoxic swelling and damage of glial cells in vitro from lactacidosis or glutamate. C6 glioma cells suspended in a physiological medium were either exposed to pH 5.0 by administration of lactic acid, or to 1 mM glutamate. Cell swelling and viability were quantified by flow cytometry. Lactacidosis of pH 5.0 led to an increase in cell volume to 139.7 ± 1.3% within 20 min whereas α-trinositol was reducing the swelling response by approximately 25% (P < 0.01). In addition, at pH 5.0 the fraction of viable cells was lowered from 94.3 ± 0.2% (control) to only 53.8 ± 3.1% after 60 min. α-Trinositol was found to protect also cell viability; at 60 min of lactacidosis 70.2 ± 1.6% of the cells still were viable (P < 0.01). The addition of glutamate (1 mM) to the cell suspension led to a steady increase in cell size, reaching 110% of control at 120 min, irrespectively of whether α-trinositol was added or not.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/brainresen_US
dc.relation.ispartofBrain Researchen_US
dc.subject.meshAcidosis, Lactic - Physiopathologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Inflammatory Agents, Non-Steroidal - Pharmacologyen_US
dc.subject.meshCell Size - Drug Effectsen_US
dc.subject.meshCell Survival - Drug Effectsen_US
dc.subject.meshGliomaen_US
dc.subject.meshGlutamic Acid - Pharmacologyen_US
dc.subject.meshInositol 1,4,5-Trisphosphate - Metabolismen_US
dc.subject.meshInositol Phosphates - Pharmacologyen_US
dc.subject.meshNeuroglia - Drug Effects - Pathologyen_US
dc.subject.meshTumor Cells, Cultured - Drug Effects - Pathologyen_US
dc.titleSwelling and damage of glial cells by lactacidosis and glutamate: Effect of α-trinositolen_US
dc.typeArticleen_US
dc.identifier.emailChang, RCC:rccchang@hkucc.hku.hken_US
dc.identifier.authorityChang, RCC=rp00470en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0006-8993(97)00751-8en_US
dc.identifier.pmid9359618-
dc.identifier.scopuseid_2-s2.0-0030931659en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030931659&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume766en_US
dc.identifier.issue1-2en_US
dc.identifier.spage285en_US
dc.identifier.epage288en_US
dc.identifier.isiWOS:A1997XX88900038-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridStaub, F=7006611117en_US
dc.identifier.scopusauthoridPeters, J=7404191248en_US
dc.identifier.scopusauthoridPlesnila, N=7003609441en_US
dc.identifier.scopusauthoridChang, RCC=7403713410en_US
dc.identifier.scopusauthoridBaethmann, A=7004994793en_US

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