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Article: Potential roles of gene expression change in adult rat spinal motoneurons following axonal injury: A comparison among c-jun, low-affinity nerve growth factor receptor (LNGFR), and nitric oxide synthase (NOS)

TitlePotential roles of gene expression change in adult rat spinal motoneurons following axonal injury: A comparison among c-jun, low-affinity nerve growth factor receptor (LNGFR), and nitric oxide synthase (NOS)
Authors
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr
Citation
Experimental Neurology, 1996, v. 141 n. 2, p. 190-200 How to Cite?
AbstractThe present study investigates expression of nitric oxide synthase (NOS), immediate early genes (IEGs, c-jun, and c-fos), and low-affinity nerve growth factor receptor (LNGFR) in adult rat spinal motoneurons in response to three conditions of axonal injury: distal axotomy, root avulsion, and root avulsion followed by a peripheral nerve (PN) graft implantation. Expression of c-jun and LNGFR were predominately observed in motoneurons of the distal axotomized segment where most motoneurons survived. In contrast, expression of NOS was exclusively found in motoneurons of the root-avulsed segment where most motoneurons died. c-fos was not expressed in motoneurons following either distal axotomy or root avulsion. In animals with PN graft implantation, a double fluorescent labeling technique was used to evaluate motoneuron regeneration. Expression of NOS was completely inhibited in all motoneurons that regenerated into the PN graft, but was not inhibited in those that did not regenerate. Moreover, regenerated motoneurons expressed LNGFR and c-jun while the nonregenerated motoneurons expressed NOS. Results of the present study have shown that motoneurons undergo changes in expression of cellular molecules in response to the axonal injury. The expression of c-jun and LNGFR may be related to the regenerative process while expression of NOS is more likely involved in the degenerative process. The results also show that PN graft implantation can alter the expression of cellular molecules and reduce motoneuron death due to root avulsion. The survival-promoting effects of PN graft implantation (presumably the effects of neurotrophic factors) may be achieved by modifying certain cellular molecules such as NOS.
Persistent Identifierhttp://hdl.handle.net/10722/149557
ISSN
2015 Impact Factor: 4.657
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWu, Wen_US
dc.date.accessioned2012-06-26T05:55:15Z-
dc.date.available2012-06-26T05:55:15Z-
dc.date.issued1996en_US
dc.identifier.citationExperimental Neurology, 1996, v. 141 n. 2, p. 190-200en_US
dc.identifier.issn0014-4886en_US
dc.identifier.urihttp://hdl.handle.net/10722/149557-
dc.description.abstractThe present study investigates expression of nitric oxide synthase (NOS), immediate early genes (IEGs, c-jun, and c-fos), and low-affinity nerve growth factor receptor (LNGFR) in adult rat spinal motoneurons in response to three conditions of axonal injury: distal axotomy, root avulsion, and root avulsion followed by a peripheral nerve (PN) graft implantation. Expression of c-jun and LNGFR were predominately observed in motoneurons of the distal axotomized segment where most motoneurons survived. In contrast, expression of NOS was exclusively found in motoneurons of the root-avulsed segment where most motoneurons died. c-fos was not expressed in motoneurons following either distal axotomy or root avulsion. In animals with PN graft implantation, a double fluorescent labeling technique was used to evaluate motoneuron regeneration. Expression of NOS was completely inhibited in all motoneurons that regenerated into the PN graft, but was not inhibited in those that did not regenerate. Moreover, regenerated motoneurons expressed LNGFR and c-jun while the nonregenerated motoneurons expressed NOS. Results of the present study have shown that motoneurons undergo changes in expression of cellular molecules in response to the axonal injury. The expression of c-jun and LNGFR may be related to the regenerative process while expression of NOS is more likely involved in the degenerative process. The results also show that PN graft implantation can alter the expression of cellular molecules and reduce motoneuron death due to root avulsion. The survival-promoting effects of PN graft implantation (presumably the effects of neurotrophic factors) may be achieved by modifying certain cellular molecules such as NOS.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnren_US
dc.relation.ispartofExperimental Neurologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAxons - Metabolismen_US
dc.subject.meshGenes, Junen_US
dc.subject.meshMaleen_US
dc.subject.meshMotor Neurons - Metabolismen_US
dc.subject.meshNitric Oxide Synthase - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Nerve Growth Factor - Metabolismen_US
dc.subject.meshSpinal Cord - Metabolismen_US
dc.titlePotential roles of gene expression change in adult rat spinal motoneurons following axonal injury: A comparison among c-jun, low-affinity nerve growth factor receptor (LNGFR), and nitric oxide synthase (NOS)en_US
dc.typeArticleen_US
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/exnr.1996.0153en_US
dc.identifier.pmid8812152-
dc.identifier.scopuseid_2-s2.0-0030271362en_US
dc.identifier.hkuros36685-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030271362&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume141en_US
dc.identifier.issue2en_US
dc.identifier.spage190en_US
dc.identifier.epage200en_US
dc.identifier.isiWOS:A1996VK39300004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWu, W=7407081122en_US

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