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Article: Functional and morphological restoration ofIntracranial brachial lesion of the retinocollicular pathway by peripheral nerve autografts in adult hamsters

TitleFunctional and morphological restoration ofIntracranial brachial lesion of the retinocollicular pathway by peripheral nerve autografts in adult hamsters
Authors
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnr
Citation
Experimental Neurology, 1996, v. 137 n. 1, p. 94-104 How to Cite?
AbstractAxons of adult mammals can regenerate through peripheral nerve grafts and restore the retinocollicular pathway if lesioned proximal to the retinal ganglion cell somata. Whether the grafting and subsequent reinnervation of the superior colliculus (SC) is possible in distal axotomy in the brain is a question of clinical relevance. We have deafferented the SC of adult hamsters at its brachium thus axotomizing the retinal ganglion cell axons rostral to its synaptic contact with the SC neurons. After unilateral brachium transection, a short segment of the autologous sciatic nerve was grafted to bridge the lesioned site to the SC (n = 28). As controls the brachium was transected and left ungrafted (n = 12). Functional restoration was examined 3 to 75 weeks later in grafted (n = 16) and control (n = 6) animals by recording visual evoked responses from the collicular cells. Prior to recording the grafts were visually evaluated and categorized into successfully (n = 8) and unsuccessfully (n = 8) grafted groups. To diffuse flash stimuli applied to the contralateral eye, visual evoked field potentials were recorded from all successfully grafted, but not in unsuccessfully grafted (with the exception of one animal) nor control animals. Unitary spike responses to diffuse flash stimuli were recorded exclusively from three successfully grafted animals. Morphological reinnervation was examined in the remaining grafted (n = 12) and control (n = 7) animals by anterogradely labeling the regenerating retinal axons with WGA-HRP. Axons in the grafts and their terminals in the superficial layers of the SC were clearly labeled in 8 of the grafted and none of the controls. From these results we conclude that the brachium of the SC is conducive to axonal regeneration and the peripheral nerve graft is indeed effective in restoring distally axotomized visual pathway in adult mammals.
Persistent Identifierhttp://hdl.handle.net/10722/149556
ISSN
2015 Impact Factor: 4.657
2015 SCImago Journal Rankings: 2.427
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSawai, Hen_US
dc.contributor.authorSugioka, Men_US
dc.contributor.authorMorigiwa, Ken_US
dc.contributor.authorMorigiwa, Ken_US
dc.contributor.authorSasaki, Hen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorFukuda, Yen_US
dc.date.accessioned2012-06-26T05:55:15Z-
dc.date.available2012-06-26T05:55:15Z-
dc.date.issued1996en_US
dc.identifier.citationExperimental Neurology, 1996, v. 137 n. 1, p. 94-104en_US
dc.identifier.issn0014-4886en_US
dc.identifier.urihttp://hdl.handle.net/10722/149556-
dc.description.abstractAxons of adult mammals can regenerate through peripheral nerve grafts and restore the retinocollicular pathway if lesioned proximal to the retinal ganglion cell somata. Whether the grafting and subsequent reinnervation of the superior colliculus (SC) is possible in distal axotomy in the brain is a question of clinical relevance. We have deafferented the SC of adult hamsters at its brachium thus axotomizing the retinal ganglion cell axons rostral to its synaptic contact with the SC neurons. After unilateral brachium transection, a short segment of the autologous sciatic nerve was grafted to bridge the lesioned site to the SC (n = 28). As controls the brachium was transected and left ungrafted (n = 12). Functional restoration was examined 3 to 75 weeks later in grafted (n = 16) and control (n = 6) animals by recording visual evoked responses from the collicular cells. Prior to recording the grafts were visually evaluated and categorized into successfully (n = 8) and unsuccessfully (n = 8) grafted groups. To diffuse flash stimuli applied to the contralateral eye, visual evoked field potentials were recorded from all successfully grafted, but not in unsuccessfully grafted (with the exception of one animal) nor control animals. Unitary spike responses to diffuse flash stimuli were recorded exclusively from three successfully grafted animals. Morphological reinnervation was examined in the remaining grafted (n = 12) and control (n = 7) animals by anterogradely labeling the regenerating retinal axons with WGA-HRP. Axons in the grafts and their terminals in the superficial layers of the SC were clearly labeled in 8 of the grafted and none of the controls. From these results we conclude that the brachium of the SC is conducive to axonal regeneration and the peripheral nerve graft is indeed effective in restoring distally axotomized visual pathway in adult mammals.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexnren_US
dc.relation.ispartofExperimental Neurologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshElectrophysiologyen_US
dc.subject.meshEvoked Potentials - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshPeripheral Nerves - Physiology - Transplantationen_US
dc.subject.meshRetinal Ganglion Cells - Physiologyen_US
dc.subject.meshSuperior Colliculi - Physiologyen_US
dc.subject.meshTransplantation, Autologousen_US
dc.titleFunctional and morphological restoration ofIntracranial brachial lesion of the retinocollicular pathway by peripheral nerve autografts in adult hamstersen_US
dc.typeArticleen_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/exnr.1996.0010en_US
dc.identifier.pmid8566216-
dc.identifier.scopuseid_2-s2.0-0030069268en_US
dc.identifier.hkuros9340-
dc.identifier.volume137en_US
dc.identifier.issue1en_US
dc.identifier.spage94en_US
dc.identifier.epage104en_US
dc.identifier.isiWOS:A1996TQ62700010-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSawai, H=7102263837en_US
dc.identifier.scopusauthoridSugioka, M=7005879198en_US
dc.identifier.scopusauthoridMorigiwa, K=36962692400en_US
dc.identifier.scopusauthoridMorigiwa, K=6603042669en_US
dc.identifier.scopusauthoridSasaki, H=7404453548en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridFukuda, Y=7402720796en_US

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