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Article: A quantitative investigation of immunocytochemically stained blood vessels in normal, benign, premalignant and malignant human oral cheek epithelium

TitleA quantitative investigation of immunocytochemically stained blood vessels in normal, benign, premalignant and malignant human oral cheek epithelium
Authors
Issue Date1995
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00428/index.htm
Citation
Virchows Archiv, 1995, v. 427 n. 2, p. 145-151 How to Cite?
AbstractThe present study was designed to determine whether increased vascularity occurs during malignant transformation of human oral cheek epithelium. Nine normal (N) samples were taken from the resection margins of benign lesions; the pathological lesions were classified as chronic inflammation (CI; n = 11), fibrous hyperplasia (FH; n = 12), lichen planus (LIP; n = 8), dysplasia (DYS; n = 5), squamous cell carcinoma (SCC; n = 25; well differentiated [SCCWD]; n = 10; moderately to poorly differentiated [SCCMPD]; n = 15) and epithelium adjacent to carcinomas (EAC; n = 6). Sections were stained with monoclonal antibody (mAb) against vimentin using an ABC immunoperoxidase technique. All blood vessels present within a depth of 0.9 mm of lamina propria were quantified irrespective of their morphology. The blood vessel parameters quantified were volume density (V(VBV, CT)), number per unit area (N(ABV, CT)), length per unit volume (L(VBV, CT)) and mean transverse sectional area (A(BV)). V(VBV, CT) increased significantly between normal and all pathological groups. Amongst the pathological groups, statistical differences were detected between CI and SCC, CI and EAC, FH and SCCWD, FH and EAC, LIP and SCC, LIP and EAC, DYS and SCCWD and DYS and EAC. The EAC group had the highest V(VBV, CT) and the values of N(ABV, CT) and L(VBV, CT) were significantly higher in all the pathological groups when compared with the normal group. No significant differences were detected between any of the pathological group. The parameter ABV increased significantly between normal and DYS, FH, SCC, EAC, FH and EAC, FH and SCC, CI and EAC, CI and SCC, LIP and EAC and LIP and SCC. Spearman rank correlations detected a positive correlation between the severity of oral lesions and all of the blood vessel parameters. We conclude that a mAb against vimentin improved the identification of smaller blood vessels and the blood vessel data suggest that angiogenesis occurs in premalignant and malignant lesions of human oral cheek epithelium. Angiogenesis seems to play an essential role in sustaining the actively growing and transforming cells.
Persistent Identifierhttp://hdl.handle.net/10722/149549
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.198
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_US
dc.contributor.authorTipoe, GLen_US
dc.contributor.authorWhite, FHen_US
dc.contributor.authorYang, Len_US
dc.date.accessioned2012-06-26T05:55:11Z-
dc.date.available2012-06-26T05:55:11Z-
dc.date.issued1995en_US
dc.identifier.citationVirchows Archiv, 1995, v. 427 n. 2, p. 145-151en_US
dc.identifier.issn0945-6317en_US
dc.identifier.urihttp://hdl.handle.net/10722/149549-
dc.description.abstractThe present study was designed to determine whether increased vascularity occurs during malignant transformation of human oral cheek epithelium. Nine normal (N) samples were taken from the resection margins of benign lesions; the pathological lesions were classified as chronic inflammation (CI; n = 11), fibrous hyperplasia (FH; n = 12), lichen planus (LIP; n = 8), dysplasia (DYS; n = 5), squamous cell carcinoma (SCC; n = 25; well differentiated [SCCWD]; n = 10; moderately to poorly differentiated [SCCMPD]; n = 15) and epithelium adjacent to carcinomas (EAC; n = 6). Sections were stained with monoclonal antibody (mAb) against vimentin using an ABC immunoperoxidase technique. All blood vessels present within a depth of 0.9 mm of lamina propria were quantified irrespective of their morphology. The blood vessel parameters quantified were volume density (V(VBV, CT)), number per unit area (N(ABV, CT)), length per unit volume (L(VBV, CT)) and mean transverse sectional area (A(BV)). V(VBV, CT) increased significantly between normal and all pathological groups. Amongst the pathological groups, statistical differences were detected between CI and SCC, CI and EAC, FH and SCCWD, FH and EAC, LIP and SCC, LIP and EAC, DYS and SCCWD and DYS and EAC. The EAC group had the highest V(VBV, CT) and the values of N(ABV, CT) and L(VBV, CT) were significantly higher in all the pathological groups when compared with the normal group. No significant differences were detected between any of the pathological group. The parameter ABV increased significantly between normal and DYS, FH, SCC, EAC, FH and EAC, FH and SCC, CI and EAC, CI and SCC, LIP and EAC and LIP and SCC. Spearman rank correlations detected a positive correlation between the severity of oral lesions and all of the blood vessel parameters. We conclude that a mAb against vimentin improved the identification of smaller blood vessels and the blood vessel data suggest that angiogenesis occurs in premalignant and malignant lesions of human oral cheek epithelium. Angiogenesis seems to play an essential role in sustaining the actively growing and transforming cells.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00428/index.htmen_US
dc.relation.ispartofVirchows Archiven_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMouth Mucosa - Blood Supplyen_US
dc.subject.meshMouth Neoplasms - Blood Supplyen_US
dc.subject.meshNeovascularization, Pathologic - Pathologyen_US
dc.subject.meshPrecancerous Conditions - Blood Supplyen_US
dc.titleA quantitative investigation of immunocytochemically stained blood vessels in normal, benign, premalignant and malignant human oral cheek epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_US
dc.identifier.authorityTipoe, GL=rp00371en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7582244-
dc.identifier.scopuseid_2-s2.0-0028808606en_US
dc.identifier.hkuros8483-
dc.identifier.volume427en_US
dc.identifier.issue2en_US
dc.identifier.spage145en_US
dc.identifier.epage151en_US
dc.identifier.isiWOS:A1995RZ18100006-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridJin, Y=26643271200en_US
dc.identifier.scopusauthoridTipoe, GL=7003550610en_US
dc.identifier.scopusauthoridWhite, FH=7202578907en_US
dc.identifier.scopusauthoridYang, L=7406279703en_US

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