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Article: Effects of male accessory sex glands on fertilization, polyspermy and morphometry of early embryos in the golden hamster

TitleEffects of male accessory sex glands on fertilization, polyspermy and morphometry of early embryos in the golden hamster
Authors
Issue Date1994
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJA
Citation
International Journal Of Andrology, 1994, v. 17 n. 2, p. 107-112 How to Cite?
AbstractPreimplantation embryos sired by hamsters without accessory sex glands (ASG) were found to have a higher mortality rate and a slower cleavage rate than those sired by sham-operated males at 72 h post coitum (p.c.). A time-course study of fertilization in vivo was conducted to determine whether this effect was due to delayed fertilization. Ultrastructural morphometry of 48 h embryos was also undertaken to establish the earliest manifestation of developmental anomalies. Compared to sham-operated controls (SH), ablation of all the ASG (TX), or just the ventral prostate (VPX) or ampullary gland (AGX) had no effect on the timing of sperm penetration, extrusion of the second polar body and pronuclear formation. Females mated with AGX males tended to have more polyspermic embryos (9.7%; p < 0.05). The volumes, volume fractions (V(V)) of the blastomere nuclei, mitochondria and yolk material of the four-cell embryos sired by these same groups of males were assessed using point counting techniques. No difference in the V(V) of yolk and mitochondria could be observed between groups. However, the SH group did have a significantly larger proportion of the cell occupied by the nucleus (p < 0.05), and the TX group had a higher proportion of the nucleus occupied by nucleoli when compared with the SH group (p < 0.01). Smaller nuclei and larger nucleoli in the TX group was interpreted as an early manifestation of a slower division rate of the blastomeres.
Persistent Identifierhttp://hdl.handle.net/10722/149548
ISSN
2014 Impact Factor: 3.695
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChow, PHen_US
dc.contributor.authorDockery, Pen_US
dc.contributor.authorCheung, MPLen_US
dc.contributor.authorO, WSen_US
dc.date.accessioned2012-06-26T05:55:10Z-
dc.date.available2012-06-26T05:55:10Z-
dc.date.issued1994en_US
dc.identifier.citationInternational Journal Of Andrology, 1994, v. 17 n. 2, p. 107-112en_US
dc.identifier.issn0105-6263en_US
dc.identifier.urihttp://hdl.handle.net/10722/149548-
dc.description.abstractPreimplantation embryos sired by hamsters without accessory sex glands (ASG) were found to have a higher mortality rate and a slower cleavage rate than those sired by sham-operated males at 72 h post coitum (p.c.). A time-course study of fertilization in vivo was conducted to determine whether this effect was due to delayed fertilization. Ultrastructural morphometry of 48 h embryos was also undertaken to establish the earliest manifestation of developmental anomalies. Compared to sham-operated controls (SH), ablation of all the ASG (TX), or just the ventral prostate (VPX) or ampullary gland (AGX) had no effect on the timing of sperm penetration, extrusion of the second polar body and pronuclear formation. Females mated with AGX males tended to have more polyspermic embryos (9.7%; p < 0.05). The volumes, volume fractions (V(V)) of the blastomere nuclei, mitochondria and yolk material of the four-cell embryos sired by these same groups of males were assessed using point counting techniques. No difference in the V(V) of yolk and mitochondria could be observed between groups. However, the SH group did have a significantly larger proportion of the cell occupied by the nucleus (p < 0.05), and the TX group had a higher proportion of the nucleus occupied by nucleoli when compared with the SH group (p < 0.01). Smaller nuclei and larger nucleoli in the TX group was interpreted as an early manifestation of a slower division rate of the blastomeres.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJAen_US
dc.relation.ispartofInternational Journal of Andrologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshEmbryo, Mammalian - Ultrastructureen_US
dc.subject.meshEmbryonic And Fetal Development - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshFertilization - Physiologyen_US
dc.subject.meshGenitalia, Male - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMesocricetusen_US
dc.subject.meshPregnancyen_US
dc.subject.meshSpermatozoa - Physiologyen_US
dc.subject.meshTime Factorsen_US
dc.titleEffects of male accessory sex glands on fertilization, polyspermy and morphometry of early embryos in the golden hamsteren_US
dc.typeArticleen_US
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_US
dc.identifier.authorityO, WS=rp00315en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8026869-
dc.identifier.scopuseid_2-s2.0-0028328709en_US
dc.identifier.hkuros7725-
dc.identifier.volume17en_US
dc.identifier.issue2en_US
dc.identifier.spage107en_US
dc.identifier.epage112en_US
dc.identifier.isiWOS:A1994NG60100008-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChow, PH=7202656919en_US
dc.identifier.scopusauthoridDockery, P=7004016702en_US
dc.identifier.scopusauthoridCheung, MPL=16749051200en_US
dc.identifier.scopusauthoridO, WS=6701729369en_US

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