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Article: Immunocytochemical localization of a growth-associated protein (GAP-43) in rat adrenal gland

TitleImmunocytochemical localization of a growth-associated protein (GAP-43) in rat adrenal gland
Authors
Issue Date1994
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 1994, v. 275 n. 3, p. 555-566 How to Cite?
AbstractWe have localized at light and electron-microscopic level the growth-associated protein GAP-43 in adrenal gland using single and double labelling immunocytochemistry. Clusters of GAP-43-immunofluorescent chromaffin cells and many immunofluorescent fibres were observed in the medulla. GAP-43-immunoreactive fibres also formed a plexus under the capsule, crossed the cortex and ramified in the zona reticulata. Double labelled sections showed the coexpression of GAP-43 with a subpopulation of tyrosine hydroxylase- and of dopamine-β-hydroxylase-immunoreactive chromaffin cells. Dual colour immunofluorescence for GAP-43 and calcitonin gene-related peptide (CGRP) revealed that some of the GAP-43-immunoreactive fibres also express CGRP. Pre-embedding electron microscopy showed GAP-43 immunoreactivity associated with the plasma membranes and cytoplasm of noradrenaline-producing chromaffin cells, and with processes of nonmyelin-forming Schwann cells. Immunoreactive unmyelinated axons and terminals were also observed. The immunostained terminals made symmetrical synaptic contacts with chromaffin cells. Immunoreactive unmyelinated fibres and small terminals were present in the cortex. Our results show that GAP-43 is expressed in noradrenergic chromaffin cells and in various types of nerve fibres that innervate the adrenal. Likely origins for these fibres include preganglionic sympathetic fibres which innervate chromaffin cells, postganglionic sympathetic fibres in the cortex, and CGRP containing sensory fibres.
Persistent Identifierhttp://hdl.handle.net/10722/149544
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLopezCosta, JJen_US
dc.contributor.authorAverill, Sen_US
dc.contributor.authorChing, YPen_US
dc.contributor.authorPriestley, JVen_US
dc.date.accessioned2012-06-26T05:55:07Z-
dc.date.available2012-06-26T05:55:07Z-
dc.date.issued1994en_US
dc.identifier.citationCell And Tissue Research, 1994, v. 275 n. 3, p. 555-566en_US
dc.identifier.issn0302-766Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/149544-
dc.description.abstractWe have localized at light and electron-microscopic level the growth-associated protein GAP-43 in adrenal gland using single and double labelling immunocytochemistry. Clusters of GAP-43-immunofluorescent chromaffin cells and many immunofluorescent fibres were observed in the medulla. GAP-43-immunoreactive fibres also formed a plexus under the capsule, crossed the cortex and ramified in the zona reticulata. Double labelled sections showed the coexpression of GAP-43 with a subpopulation of tyrosine hydroxylase- and of dopamine-β-hydroxylase-immunoreactive chromaffin cells. Dual colour immunofluorescence for GAP-43 and calcitonin gene-related peptide (CGRP) revealed that some of the GAP-43-immunoreactive fibres also express CGRP. Pre-embedding electron microscopy showed GAP-43 immunoreactivity associated with the plasma membranes and cytoplasm of noradrenaline-producing chromaffin cells, and with processes of nonmyelin-forming Schwann cells. Immunoreactive unmyelinated axons and terminals were also observed. The immunostained terminals made symmetrical synaptic contacts with chromaffin cells. Immunoreactive unmyelinated fibres and small terminals were present in the cortex. Our results show that GAP-43 is expressed in noradrenergic chromaffin cells and in various types of nerve fibres that innervate the adrenal. Likely origins for these fibres include preganglionic sympathetic fibres which innervate chromaffin cells, postganglionic sympathetic fibres in the cortex, and CGRP containing sensory fibres.en_US
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_US
dc.relation.ispartofCell and Tissue Researchen_US
dc.subject.meshAdrenal Glands - Chemistry - Cytology - Innervationen_US
dc.subject.meshAdrenal Medulla - Chemistry - Innervationen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodiesen_US
dc.subject.meshCalcitonin Gene-Related Peptide - Analysisen_US
dc.subject.meshDopamine Beta-Hydroxylase - Analysisen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshGap-43 Proteinen_US
dc.subject.meshMembrane Glycoproteins - Analysisen_US
dc.subject.meshMicroscopy, Immunoelectronen_US
dc.subject.meshNerve Fibers - Chemistryen_US
dc.subject.meshNerve Tissue Proteins - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshSynapses - Ultrastructureen_US
dc.subject.meshTyrosine 3-Monooxygenase - Analysisen_US
dc.titleImmunocytochemical localization of a growth-associated protein (GAP-43) in rat adrenal glanden_US
dc.typeArticleen_US
dc.identifier.emailChing, YP:ypching@hku.hken_US
dc.identifier.authorityChing, YP=rp00469en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7907951-
dc.identifier.scopuseid_2-s2.0-0028118392en_US
dc.identifier.volume275en_US
dc.identifier.issue3en_US
dc.identifier.spage555en_US
dc.identifier.epage566en_US
dc.identifier.isiWOS:A1994MW36300015-
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridLopezCosta, JJ=6701319980en_US
dc.identifier.scopusauthoridAverill, S=7003816024en_US
dc.identifier.scopusauthoridChing, YP=7005431277en_US
dc.identifier.scopusauthoridPriestley, JV=7006451459en_US

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