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- Publisher Website: 10.1007/BF00318824
- Scopus: eid_2-s2.0-0028118392
- PMID: 7907951
- WOS: WOS:A1994MW36300015
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Article: Immunocytochemical localization of a growth-associated protein (GAP-43) in rat adrenal gland
Title | Immunocytochemical localization of a growth-associated protein (GAP-43) in rat adrenal gland |
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Authors | |
Keywords | Adrenal Autonomic nervous system Electron microscopy GAP-43 Immunohistochemistry Rat (Wistar) Schwann cells Tyrosine hydroxylase |
Issue Date | 1994 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm |
Citation | Cell And Tissue Research, 1994, v. 275 n. 3, p. 555-566 How to Cite? |
Abstract | We have localized at light and electron-microscopic level the growth-associated protein GAP-43 in adrenal gland using single and double labelling immunocytochemistry. Clusters of GAP-43-immunofluorescent chromaffin cells and many immunofluorescent fibres were observed in the medulla. GAP-43-immunoreactive fibres also formed a plexus under the capsule, crossed the cortex and ramified in the zona reticulata. Double labelled sections showed the coexpression of GAP-43 with a subpopulation of tyrosine hydroxylase- and of dopamine-β-hydroxylase-immunoreactive chromaffin cells. Dual colour immunofluorescence for GAP-43 and calcitonin gene-related peptide (CGRP) revealed that some of the GAP-43-immunoreactive fibres also express CGRP. Pre-embedding electron microscopy showed GAP-43 immunoreactivity associated with the plasma membranes and cytoplasm of noradrenaline-producing chromaffin cells, and with processes of nonmyelin-forming Schwann cells. Immunoreactive unmyelinated axons and terminals were also observed. The immunostained terminals made symmetrical synaptic contacts with chromaffin cells. Immunoreactive unmyelinated fibres and small terminals were present in the cortex. Our results show that GAP-43 is expressed in noradrenergic chromaffin cells and in various types of nerve fibres that innervate the adrenal. Likely origins for these fibres include preganglionic sympathetic fibres which innervate chromaffin cells, postganglionic sympathetic fibres in the cortex, and CGRP containing sensory fibres. |
Persistent Identifier | http://hdl.handle.net/10722/149544 |
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.965 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | LopezCosta, JJ | en_US |
dc.contributor.author | Averill, S | en_US |
dc.contributor.author | Ching, YP | en_US |
dc.contributor.author | Priestley, JV | en_US |
dc.date.accessioned | 2012-06-26T05:55:07Z | - |
dc.date.available | 2012-06-26T05:55:07Z | - |
dc.date.issued | 1994 | en_US |
dc.identifier.citation | Cell And Tissue Research, 1994, v. 275 n. 3, p. 555-566 | en_US |
dc.identifier.issn | 0302-766X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/149544 | - |
dc.description.abstract | We have localized at light and electron-microscopic level the growth-associated protein GAP-43 in adrenal gland using single and double labelling immunocytochemistry. Clusters of GAP-43-immunofluorescent chromaffin cells and many immunofluorescent fibres were observed in the medulla. GAP-43-immunoreactive fibres also formed a plexus under the capsule, crossed the cortex and ramified in the zona reticulata. Double labelled sections showed the coexpression of GAP-43 with a subpopulation of tyrosine hydroxylase- and of dopamine-β-hydroxylase-immunoreactive chromaffin cells. Dual colour immunofluorescence for GAP-43 and calcitonin gene-related peptide (CGRP) revealed that some of the GAP-43-immunoreactive fibres also express CGRP. Pre-embedding electron microscopy showed GAP-43 immunoreactivity associated with the plasma membranes and cytoplasm of noradrenaline-producing chromaffin cells, and with processes of nonmyelin-forming Schwann cells. Immunoreactive unmyelinated axons and terminals were also observed. The immunostained terminals made symmetrical synaptic contacts with chromaffin cells. Immunoreactive unmyelinated fibres and small terminals were present in the cortex. Our results show that GAP-43 is expressed in noradrenergic chromaffin cells and in various types of nerve fibres that innervate the adrenal. Likely origins for these fibres include preganglionic sympathetic fibres which innervate chromaffin cells, postganglionic sympathetic fibres in the cortex, and CGRP containing sensory fibres. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm | en_US |
dc.relation.ispartof | Cell and Tissue Research | en_US |
dc.subject | Adrenal | - |
dc.subject | Autonomic nervous system | - |
dc.subject | Electron microscopy | - |
dc.subject | GAP-43 | - |
dc.subject | Immunohistochemistry | - |
dc.subject | Rat (Wistar) | - |
dc.subject | Schwann cells | - |
dc.subject | Tyrosine hydroxylase | - |
dc.subject.mesh | Adrenal Glands - Chemistry - Cytology - Innervation | en_US |
dc.subject.mesh | Adrenal Medulla - Chemistry - Innervation | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Antibodies | en_US |
dc.subject.mesh | Calcitonin Gene-Related Peptide - Analysis | en_US |
dc.subject.mesh | Dopamine Beta-Hydroxylase - Analysis | en_US |
dc.subject.mesh | Fluorescent Antibody Technique | en_US |
dc.subject.mesh | Gap-43 Protein | en_US |
dc.subject.mesh | Membrane Glycoproteins - Analysis | en_US |
dc.subject.mesh | Microscopy, Immunoelectron | en_US |
dc.subject.mesh | Nerve Fibers - Chemistry | en_US |
dc.subject.mesh | Nerve Tissue Proteins - Analysis | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Wistar | en_US |
dc.subject.mesh | Synapses - Ultrastructure | en_US |
dc.subject.mesh | Tyrosine 3-Monooxygenase - Analysis | en_US |
dc.title | Immunocytochemical localization of a growth-associated protein (GAP-43) in rat adrenal gland | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ching, YP:ypching@hku.hk | en_US |
dc.identifier.authority | Ching, YP=rp00469 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/BF00318824 | - |
dc.identifier.pmid | 7907951 | - |
dc.identifier.scopus | eid_2-s2.0-0028118392 | en_US |
dc.identifier.volume | 275 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 555 | en_US |
dc.identifier.epage | 566 | en_US |
dc.identifier.isi | WOS:A1994MW36300015 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | LopezCosta, JJ=6701319980 | en_US |
dc.identifier.scopusauthorid | Averill, S=7003816024 | en_US |
dc.identifier.scopusauthorid | Ching, YP=7005431277 | en_US |
dc.identifier.scopusauthorid | Priestley, JV=7006451459 | en_US |
dc.identifier.issnl | 0302-766X | - |