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Article: Neuronal nitric oxide synthase is induced in spinal neurons by traumatic injury

TitleNeuronal nitric oxide synthase is induced in spinal neurons by traumatic injury
Authors
Issue Date1994
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 1994, v. 61 n. 4, p. 719-726 How to Cite?
AbstractNitric oxide appears to mediate the immune functions of macrophages, the influence of endothelial cells on blood vessel relaxation, and also to serve as a neurotransmitter in the central and peripheral nervous system. Macrophage nitric oxide synthase is inducible with massive increases in new nitric oxide synthase protein synthesis following immune stimulation of macrophages? By contrast, endothelial nitric oxide synthase and neuronal nitric oxide synthase are thought to be constitutive with activation induced by calcium entry into cells in the absence of new protein synthesis. Developmental studies showing the transient expression of neuronal nitric oxide synthase in embryonic and early postnatal life in rodent spinal motoneurons and cerebral cortical plate neurons (Bredt and Snyder, unpublished observations) implies inducibility of neuronal nitric oxide synthase. Moreover, neuronaI nitric oxide synthase expression is greatly enhanced in sensory ganglia following peripheral axotomy. Staining for NADPH diaphorase in spinal motoneurons is greatly increased following ventral root avulsion. In many parts of the Central Nervous System NADPH diaphorase staining reflects nitric oxide synthase. In the present study, we have combined in situ hybridization for neuronal nitric oxide synthase, immunohistochemical staining of neuronal nitric oxide synthase, and NADPH diaphorase staining to establish that neuronal nitric oxide synthase expression is markedly augmented in spinal motoneurons following avulsion. The generality of this effect is evident from augmented staining in nucleus dorsalis following spinal cord transection.
Persistent Identifierhttp://hdl.handle.net/10722/149543
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWu, Wen_US
dc.date.accessioned2012-06-26T05:55:06Z-
dc.date.available2012-06-26T05:55:06Z-
dc.date.issued1994en_US
dc.identifier.citationNeuroscience, 1994, v. 61 n. 4, p. 719-726en_US
dc.identifier.issn0306-4522en_US
dc.identifier.urihttp://hdl.handle.net/10722/149543-
dc.description.abstractNitric oxide appears to mediate the immune functions of macrophages, the influence of endothelial cells on blood vessel relaxation, and also to serve as a neurotransmitter in the central and peripheral nervous system. Macrophage nitric oxide synthase is inducible with massive increases in new nitric oxide synthase protein synthesis following immune stimulation of macrophages? By contrast, endothelial nitric oxide synthase and neuronal nitric oxide synthase are thought to be constitutive with activation induced by calcium entry into cells in the absence of new protein synthesis. Developmental studies showing the transient expression of neuronal nitric oxide synthase in embryonic and early postnatal life in rodent spinal motoneurons and cerebral cortical plate neurons (Bredt and Snyder, unpublished observations) implies inducibility of neuronal nitric oxide synthase. Moreover, neuronaI nitric oxide synthase expression is greatly enhanced in sensory ganglia following peripheral axotomy. Staining for NADPH diaphorase in spinal motoneurons is greatly increased following ventral root avulsion. In many parts of the Central Nervous System NADPH diaphorase staining reflects nitric oxide synthase. In the present study, we have combined in situ hybridization for neuronal nitric oxide synthase, immunohistochemical staining of neuronal nitric oxide synthase, and NADPH diaphorase staining to establish that neuronal nitric oxide synthase expression is markedly augmented in spinal motoneurons following avulsion. The generality of this effect is evident from augmented staining in nucleus dorsalis following spinal cord transection.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_US
dc.relation.ispartofNeuroscienceen_US
dc.subject.meshAmino Acid Oxidoreductases - Biosynthesisen_US
dc.subject.meshAnimalsen_US
dc.subject.meshEnzyme Induction - Physiologyen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMaleen_US
dc.subject.meshMotor Neurons - Enzymologyen_US
dc.subject.meshNeurons - Enzymologyen_US
dc.subject.meshNitric Oxide Synthaseen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshSpinal Cord Injuries - Enzymology - Pathologyen_US
dc.titleNeuronal nitric oxide synthase is induced in spinal neurons by traumatic injuryen_US
dc.typeArticleen_US
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0306-4522(94)90394-8en_US
dc.identifier.pmid7530816-
dc.identifier.scopuseid_2-s2.0-0028048108en_US
dc.identifier.volume61en_US
dc.identifier.issue4en_US
dc.identifier.spage719en_US
dc.identifier.epage726en_US
dc.identifier.isiWOS:A1994PE79700001-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridWu, W=7407081122en_US

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