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Article: Effects of cis-4-hydroxy-L-proline on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pig

TitleEffects of cis-4-hydroxy-L-proline on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pig
Authors
Issue Date1993
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 1993, v. 23 n. 4, p. 337-354 How to Cite?
AbstractThe present study examined the effects of cis-4-hydroxy-L-proline (CHP), a proline analog, on the androgen-induced growth of the lateral prostate of prepubertally castrated guinea pigs. Prepubertal male guinea pigs were castrated at the age of 3 weeks and allowed to recover completely before subjection to an experimental regime to alter the stromal collagen synthesis by CHP. The animals were kept on a special proline-deficient diet (PDD) for a week before the subcutaneous injection of CHP (200 mg/kg/day) for 3 days, followed by a combined injection of CHP and dihydrotestosterone (DHT) (10 mg/kg/day) for 10 days. Control animals were injected with saline and DHT only. At the end of the experiment, the lateral prostate was removed and examined by 1) conventional transmission electron microscopy (TEM), 2) staining of proteoglycans (PGs) by Cuprolinic Blue (CB) using the critical electrolyte concentration (CEC) method, 3) carbohydrate and lectin histochemistry, and 4) electron microscopy (EM) lectin-gold labelling. The results showed that the wet weight of prostate from CHP-treated animals was significantly lower than the control and recovery groups. The epithelium was low columnar with an obvious increase in intercellular spaces and number of basal cells. The glandular cells showed little secretory activity with a decrease in number of granular endoplasmic reticulum (GER) profiles, secretory granules, and a small Golgi apparatus. The stroma was composed of stromal cells separated by large intercellular spaces with very sparse collagen fibrils, and a decrease in stromal PGs especially those PGs normally associated with collagen fibrils. CHP treatment also caused perturbation and disorganization in the epithelial basement membrane. The results suggested that stromal collagen is essential in mediating the response of glandular cells to DHT stimulation. Defective stromal collagens hamper the responsiveness of prostatic gland to androgen.
Persistent Identifierhttp://hdl.handle.net/10722/149540
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, YCen_US
dc.contributor.authorChan, FLen_US
dc.date.accessioned2012-06-26T05:55:05Z-
dc.date.available2012-06-26T05:55:05Z-
dc.date.issued1993en_US
dc.identifier.citationProstate, 1993, v. 23 n. 4, p. 337-354en_US
dc.identifier.issn0270-4137en_US
dc.identifier.urihttp://hdl.handle.net/10722/149540-
dc.description.abstractThe present study examined the effects of cis-4-hydroxy-L-proline (CHP), a proline analog, on the androgen-induced growth of the lateral prostate of prepubertally castrated guinea pigs. Prepubertal male guinea pigs were castrated at the age of 3 weeks and allowed to recover completely before subjection to an experimental regime to alter the stromal collagen synthesis by CHP. The animals were kept on a special proline-deficient diet (PDD) for a week before the subcutaneous injection of CHP (200 mg/kg/day) for 3 days, followed by a combined injection of CHP and dihydrotestosterone (DHT) (10 mg/kg/day) for 10 days. Control animals were injected with saline and DHT only. At the end of the experiment, the lateral prostate was removed and examined by 1) conventional transmission electron microscopy (TEM), 2) staining of proteoglycans (PGs) by Cuprolinic Blue (CB) using the critical electrolyte concentration (CEC) method, 3) carbohydrate and lectin histochemistry, and 4) electron microscopy (EM) lectin-gold labelling. The results showed that the wet weight of prostate from CHP-treated animals was significantly lower than the control and recovery groups. The epithelium was low columnar with an obvious increase in intercellular spaces and number of basal cells. The glandular cells showed little secretory activity with a decrease in number of granular endoplasmic reticulum (GER) profiles, secretory granules, and a small Golgi apparatus. The stroma was composed of stromal cells separated by large intercellular spaces with very sparse collagen fibrils, and a decrease in stromal PGs especially those PGs normally associated with collagen fibrils. CHP treatment also caused perturbation and disorganization in the epithelial basement membrane. The results suggested that stromal collagen is essential in mediating the response of glandular cells to DHT stimulation. Defective stromal collagens hamper the responsiveness of prostatic gland to androgen.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_US
dc.relation.ispartofProstateen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBasement Membrane - Drug Effectsen_US
dc.subject.meshCollagen - Analysis - Physiologyen_US
dc.subject.meshDihydrotestosterone - Pharmacologyen_US
dc.subject.meshEpithelium - Drug Effectsen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshHydroxyproline - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshOrchiectomyen_US
dc.subject.meshPeriodic Acid-Schiff Reactionen_US
dc.subject.meshProstate - Drug Effects - Growth & Development - Ultrastructureen_US
dc.subject.meshProteoglycans - Analysisen_US
dc.subject.meshSexual Maturationen_US
dc.titleEffects of cis-4-hydroxy-L-proline on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pigen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pros.2990230407en_US
dc.identifier.pmid8259345-
dc.identifier.scopuseid_2-s2.0-0027714817en_US
dc.identifier.volume23en_US
dc.identifier.issue4en_US
dc.identifier.spage337en_US
dc.identifier.epage354en_US
dc.identifier.isiWOS:A1993MM57900006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, YC=7403041798en_US
dc.identifier.scopusauthoridChan, FL=7202586505en_US

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