File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Thiamine pyrophosphatase activity in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with oestradiol

TitleThiamine pyrophosphatase activity in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with oestradiol
Authors
Issue Date1993
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1567-2379
Citation
Histochemical Journal, 1993, v. 25 n. 1, p. 77-85 How to Cite?
AbstractUltrastructural localization of thiamine pyrophosphatase (TPPase) activity was studied in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with 17β-oestradiol benzoate. The present study has demonstrated that TPPase reaction product is consistently localized in the three to four trans cisternae of Golgi complexes in both the lateral prostate and the seminal vesicle. The reaction was intense and the reaction product often filled the cisternae completely. After castration there was a decrease in TPPase activity in both glands as revealed by the reduction in the amount of the reaction product which was found mainly in one to two trans cisternae of the regressed Golgi complex. The reaction product changed from a dense to a more particulate or granular pattern or to discrete deposits of high electron-density. Administration of 17β-oestradiol benzoate to the castrates caused changes in the localization and patterns of distribution of TPPase. In the lateral prostate there was an apparent increase in TPPase activity. The reaction product was found in two to four trans cisternae and occasionally in the trans-most cisternae of the dilated Golgi complex. The reaction product appeared as discrete, dense coarse precipitates. In the seminal vesicle TPPase reaction product was consistently found in one to two trans cisternae in cells with larger Golgi complexes. However, almost all cisternae of the smaller Golgi complexes were TPPase-positive. The cytochemical results of the present study suggest that TPPase activity and possibly the process of glycosylation in secretory cells of the lateral prostate and seminal vesicle may have been affected after castration and after oestradiol administration.
Persistent Identifierhttp://hdl.handle.net/10722/149538
ISSN
2004 Impact Factor: 0.928
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTam, CCen_US
dc.contributor.authorWong, YCen_US
dc.date.accessioned2012-06-26T05:55:04Z-
dc.date.available2012-06-26T05:55:04Z-
dc.date.issued1993en_US
dc.identifier.citationHistochemical Journal, 1993, v. 25 n. 1, p. 77-85en_US
dc.identifier.issn0018-2214en_US
dc.identifier.urihttp://hdl.handle.net/10722/149538-
dc.description.abstractUltrastructural localization of thiamine pyrophosphatase (TPPase) activity was studied in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with 17β-oestradiol benzoate. The present study has demonstrated that TPPase reaction product is consistently localized in the three to four trans cisternae of Golgi complexes in both the lateral prostate and the seminal vesicle. The reaction was intense and the reaction product often filled the cisternae completely. After castration there was a decrease in TPPase activity in both glands as revealed by the reduction in the amount of the reaction product which was found mainly in one to two trans cisternae of the regressed Golgi complex. The reaction product changed from a dense to a more particulate or granular pattern or to discrete deposits of high electron-density. Administration of 17β-oestradiol benzoate to the castrates caused changes in the localization and patterns of distribution of TPPase. In the lateral prostate there was an apparent increase in TPPase activity. The reaction product was found in two to four trans cisternae and occasionally in the trans-most cisternae of the dilated Golgi complex. The reaction product appeared as discrete, dense coarse precipitates. In the seminal vesicle TPPase reaction product was consistently found in one to two trans cisternae in cells with larger Golgi complexes. However, almost all cisternae of the smaller Golgi complexes were TPPase-positive. The cytochemical results of the present study suggest that TPPase activity and possibly the process of glycosylation in secretory cells of the lateral prostate and seminal vesicle may have been affected after castration and after oestradiol administration.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1567-2379en_US
dc.relation.ispartofHistochemical Journalen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCastrationen_US
dc.subject.meshEstradiol - Analogs & Derivatives - Pharmacologyen_US
dc.subject.meshGolgi Apparatus - Enzymology - Ultrastructureen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshProstate - Drug Effects - Enzymology - Ultrastructureen_US
dc.subject.meshSeminal Vesicles - Drug Effects - Enzymology - Ultrastructureen_US
dc.subject.meshThiamine Pyrophosphatase - Metabolismen_US
dc.titleThiamine pyrophosphatase activity in secretory cells of the lateral prostate and seminal vesicle of normal and castrated guinea pigs and castrates treated with oestradiolen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8432665-
dc.identifier.scopuseid_2-s2.0-0027512926en_US
dc.identifier.volume25en_US
dc.identifier.issue1en_US
dc.identifier.spage77en_US
dc.identifier.epage85en_US
dc.identifier.isiWOS:A1993KK50500009-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridTam, CC=7201442974en_US
dc.identifier.scopusauthoridWong, YC=7403041798en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats