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Article: Identification of differentially expressed RNA in human ovarian carcinoma cells by arbitrarily primed PCR fingerprinting of total RNAs

TitleIdentification of differentially expressed RNA in human ovarian carcinoma cells by arbitrarily primed PCR fingerprinting of total RNAs
Authors
Issue Date1993
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 1993, v. 3 n. 1, p. 13-17 How to Cite?
AbstractUsing arbitrarily primed PCR fingerprinting of RNA (RAP), we have analyzed RNAs prepared from two normal ovarian surface epithelial cell cultures, two normal mesothelial cell cultures, and eight independent ovarian carcinoma cell lines. Each arbitrarily chosen primer gave a unique fingerprint of about 30 cDNA products. Most of the cDNA products produced by any particular primer were shared between all cell lines. However, one primer detected a cDNA PCR product that was absent in all eight ovarian carcinoma cell lines but present in all normal cell cultures. We have cloned and sequenced the DNA fragment that distinguishes normal from ovarian carcinoma cell lines. The DNA sequence has one continuous open reading frame throughout which indicates that it may be from a translated gene. Furthermore, we confirmed the differential expression of the gene by Northern blot analysis. We also observed that the intensity of the band in the RNA fingerprint correlates with the expression level of the corresponding RNA. These results further demonstrate the ability of RAP to detect differentially expressed genes in a quantitative manner and demonstrated the application of RAP for the detection of differential gene expression during carcinogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/149536
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270

 

DC FieldValueLanguage
dc.contributor.authorWong, KKen_US
dc.contributor.authorMok, CHen_US
dc.contributor.authorWelsh, JTen_US
dc.contributor.authorMcclelland, Men_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorBerkowitz, RSen_US
dc.contributor.authorMok, SCen_US
dc.date.accessioned2012-06-26T05:54:57Z-
dc.date.available2012-06-26T05:54:57Z-
dc.date.issued1993en_US
dc.identifier.citationInternational Journal Of Oncology, 1993, v. 3 n. 1, p. 13-17en_US
dc.identifier.issn1019-6439en_US
dc.identifier.urihttp://hdl.handle.net/10722/149536-
dc.description.abstractUsing arbitrarily primed PCR fingerprinting of RNA (RAP), we have analyzed RNAs prepared from two normal ovarian surface epithelial cell cultures, two normal mesothelial cell cultures, and eight independent ovarian carcinoma cell lines. Each arbitrarily chosen primer gave a unique fingerprint of about 30 cDNA products. Most of the cDNA products produced by any particular primer were shared between all cell lines. However, one primer detected a cDNA PCR product that was absent in all eight ovarian carcinoma cell lines but present in all normal cell cultures. We have cloned and sequenced the DNA fragment that distinguishes normal from ovarian carcinoma cell lines. The DNA sequence has one continuous open reading frame throughout which indicates that it may be from a translated gene. Furthermore, we confirmed the differential expression of the gene by Northern blot analysis. We also observed that the intensity of the band in the RNA fingerprint correlates with the expression level of the corresponding RNA. These results further demonstrate the ability of RAP to detect differentially expressed genes in a quantitative manner and demonstrated the application of RAP for the detection of differential gene expression during carcinogenesis.en_US
dc.languageengen_US
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_US
dc.relation.ispartofInternational Journal of Oncologyen_US
dc.titleIdentification of differentially expressed RNA in human ovarian carcinoma cells by arbitrarily primed PCR fingerprinting of total RNAsen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0027430918en_US
dc.identifier.hkuros7754-
dc.identifier.volume3en_US
dc.identifier.issue1en_US
dc.identifier.spage13en_US
dc.identifier.epage17en_US
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridWong, KK=37096551700en_US
dc.identifier.scopusauthoridMok, CH=7102344233en_US
dc.identifier.scopusauthoridWelsh, JT=7202711374en_US
dc.identifier.scopusauthoridMcClelland, M=7103047213en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridBerkowitz, RS=7201352221en_US
dc.identifier.scopusauthoridMok, SC=7006015487en_US

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